LOCUS       pNIMXR-rtTA             7502 bp    DNA     circular     19-JAN-2018
DEFINITION  LMBP 10517.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12122666|
COMMENT     VNTDBDATE|-12122666|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNIMXR-rtTA|

COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pNIMXR-rtTA" 0 7502 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "SacI" "KpnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 654 659 1 7502 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 659 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 5301 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "T7 promoter" 30 0 0 627 643 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "627..643") 
COMMENT        #3=(CFSignal (CObList) "T3 promoter" 30 0 1 5316 5332 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6651..6667)") 
COMMENT        #4=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 21 0 1 5358 5395 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6693..6730)") 
COMMENT        #5=(CFSignal (CObList) "lac promoter" 30 0 1 5396 5479 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6731..6814)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 5571 5973 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(6906..7308)") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6514 7374 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(7849..8709)") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 1 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4..459)") 
COMMENT        #9=(CFSignal (CObList) "cartTA" 4 0 0 1213 2196 0 (CStringList) 
COMMENT            (CStringList "/gene=\"cartTA\"" "/codon_start=1" "/transl_table=12" 
COMMENT             "/product=\"reverse tetracycline regulated transactivator\"" 
COMMENT             "/protein_id=\"ART66077.1\"") 1 1 1 1 "560..1543") 
COMMENT        #10=(CFSignal (CObList #11=(CLocSeg 0 562) #12=(CLocSeg 1217 9)) "SAT1" 
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COMMENT             (CStringList "/gene=\"SAT1\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"SAT1\"" "/protein_id=\"ART66078.1\"") 1 1 1 1 
COMMENT             "complement(join(1925..2487,3142..3151))") 
COMMENT        #13=(CFSignal (CObList) "CaRP10 promoter" 29 0 0 668 1212 0 
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COMMENT             1 "15..559") 
COMMENT        #14=(CFSignal (CObList) "CaQCR7 terminator" 43 0 0 2217 2570 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "1564..1917") 
COMMENT        #15=(CFSignal (CObList) "CaRP10 terminator" 43 0 0 4777 5278 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "4124..4625") 
COMMENT        #16=(CFSignal (CObList) "CaACT1 promoter" 29 0 1 3805 4302 0 
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COMMENT             1 "3152..3649") 
COMMENT        #17=(CFSignal (CObList) "CaACT1 terminator" 43 0 1 4309 4696 0 
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COMMENT             1 1 "3656..4043")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
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COMMENT       (CTextView 0 
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COMMENT                          "DNA Plasmid Phage 'pNIMXR-rtTA'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 10517." 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 01/19/18 10:41" 1) 
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COMMENT                          "Last Modified: 01/19/18 10:41" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7502 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-JAN-2018" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  cartTA;  CaSAT1;  GFP" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "CaACT1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 -15.6229 -5.22699 0 -10 4.88388 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -15.3434 1.74233 0 -10 3.56994 1.16519 
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COMMENT                                   4.42016) 
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COMMENT                                   "CaQCR7 terminator" "@N" 1 16.7767 -5.22699 0 
COMMENT                                   -10 7.71008 1.16519 #293#) 
COMMENT                             #295=(CLine 
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COMMENT                                   0 -10 7.46217 1.16519 #295#) 
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FEATURES             Location/Qualifiers
     promoter        627..643
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(5316..5332)
                     /vntifkey="30"
                     /label=T3\promoter
     misc_feature    complement(5358..5395)
                     /vntifkey="21"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(5396..5479)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(5571..5973)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6514..7374)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4..459)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        668..1212
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaRP10\promoter
     CDS             1213..2196
                     /gene="cartTA"
                     /codon_start=1
                     /transl_table=12
                     /product="reverse tetracycline regulated transactivator"
                     /protein_id="ART66077.1"
                     /vntifkey="4"
                     /label=cartTA
     terminator      2217..2570
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaQCR7\terminator
     CDS             complement(2578..3140,3795..3804)
                     /gene="SAT1"
                     /codon_start=1
                     /transl_table=12
                     /product="SAT1"
                     /protein_id="ART66078.1"
                     /vntifkey="4"
                     /label=SAT1
     promoter        complement(3805..4302)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaACT1\promoter
     terminator      complement(4309..4696)
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaACT1\terminator
     terminator      4777..5278
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaRP10\terminator
BASE COUNT     2307 a      1479 c      1560 g      2156 t 
ORIGIN
        1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 
      661 accgcggtca gtagatgatt tgttgttgtc gtttgatttt gttacagcgt aaccagtgcg 
      721 ttttgtttgt ttccacatca tacacttcac tgaaactaaa taagtttgtt tacattttga 
      781 gacttcaggt acgacccagg gttgcgacaa agtttaggta gtttgtcgtc tgaatgtcgc 
      841 aacaaaatag ggctgtagcc ctagtcatgt gatgtgaatt aacataacaa gaagaattgc 
      901 tggtgcgcaa aaagattatg tgtattttat gtgcgttgtt atcctgcaca ctaaaattga 
      961 gcagtgtaca cacacacata ttgggctgta tttttatttt tgtttttctg ctgttctctc 
     1021 actgttaagc tctaagtgaa tttgtgtgtg ctgtaatagt gtgtgtgttc caagtcccag 
     1081 ctctcacaga tactcacgca cgcccatact actgaaaatt tcctgacttt ctgtatctaa 
     1141 aaatttttta ctaggaattt ttttctttta cgtttttcac ttgtttcata taatcaccaa 
     1201 ctcaagtaca acatgtctag attagataaa agtaaagtga ttaacggcgc attagagttg 
     1261 cttaatgggg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta 
     1321 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttacca 
     1381 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa 
     1441 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga 
     1501 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 
     1561 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgca 
     1621 gtggggcatt ttactttagg ttgcgtattg gaagaacaag agcatcaagt cgctaaagaa 
     1681 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 
     1741 tttgatagac aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 
     1801 ttagaaaaac aacttaaatg tgaaagtggg tcctcgactt tgtttggtgg cgccaatttt 
     1861 aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa cagtagcaac 
     1921 ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc 
     1981 tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat tgatgatggt 
     2041 aataattcaa aaccattgtc acctggttgg acggaccaaa ctgcgtataa cgcgtttgga 
     2101 atcactacag ggatgtttaa taccactaca atggatgatg tatataacta tctattcgat 
     2161 gatgaagata ccccaccaaa cccaaaaaaa gagtaaggat ctaaattctg gaaatctaag 
     2221 taagtaagag gagtaggagg aggaagaaga tgagaaggtc aaacattgaa ataggaaaca 
     2281 agaaagcagg agacaagttt aaagaatgaa taataaatat tcttatgaaa gttatattta 
     2341 tttttgttag ttttctttta aatatattcg taatgtcaat ataaaacaat ttacaaaaag 
     2401 agattgtcaa aaagaagaaa cacatattat aatctaagtc tattaaatat ctctataaaa 
     2461 aaataatgtt atacttgaac aaaagcacta ttctttttgt ttatctatat ctatatatct 
     2521 atctatcaaa tatctttgtt tttaatctga tcgactcatt cctaatttaa acatatgtta 
     2581 ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt tcgagacttg 
     2641 aggtctagtt ttatacgtga agaggtcaat gccgccgaga gtaaagccac attttgcgta 
     2701 caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa ggagctgtct 
     2761 gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt tgcctcggtg 
     2821 cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg agttgagttc 
     2881 aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt cttcatcaga 
     2941 gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag atagttcaaa 
     3001 gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt tctcagcatc 
     3061 caatgtttcc gccacctgct cagggatcac cgaaattttc atatgagaac cgttatcgat 
     3121 aactaaagca gcaacttctt ctataaaaat gggttagtat gacagtcatt taaataagga 
     3181 atttttcagt tggcttggtt tcaattcaat gttcgttttt tttttttctt gctgtgtttg 
     3241 tgtttgtgtt gtttatagtt gtgtgcactg atcgtcgaaa aaaaaaattc atagtgagcc 
     3301 gggaaatctg tatagcccag ataacaacac aagtccaaac tagaaactcg tcaaacacca 
     3361 aaagcaatgt tgaatcaatt gccttgcaca agtacacgta ggaaaacata aaacattgca 
     3421 attttgaata ttgagccttt tgtcgtaaca ttgattgata ggattactca ccgaatggtt 
     3481 ttgaaaccac tgccgacaga tcaatcaatc aatcaaaaaa cgtgaacttt gaaaaagggg 
     3541 aagaacagat acattgaagt tagccatttc cactgatcgt cacaacatat ctgataaatt 
     3601 actttcaaaa ttataagctg atgtgtgtgt attattaatg tgacagtaac atcccaaacg 
     3661 agaaatatta tctcgacaac aaaaaagttt gatctgaatt gaaaatgaag ttttcccacc 
     3721 ctacccattt gtcatattga aaccaatcaa ctgattaatc aatcaattag aattgaagct 
     3781 aaactaaaac ataccaccgt ccattttgaa tgattatatt tttttaatat taatatcgag 
     3841 ataatgtttc taagaaagaa agaaaaccag gagtgaaaat tagaaaagga aaggaaagga 
     3901 aaaaaagaaa aatctgaaaa tatataaaaa aaaattgttt cgttggcaat aaatcttggt 
     3961 gagaacagcg accgaaagca aataagaaca aaatatgagt gtattacgtt gaacaactaa 
     4021 ttaacgtgtg tgtatggatc tttttttctt ttttctcttt aaccgactat aaacaacaaa 
     4081 catttttggg cagtgcacac actacttaat atacacagca taaattacac gattagaaac 
     4141 aaattagctt attaaaataa cctaatcaaa ccgaatattt tatggtatta tgagtaaact 
     4201 atataatata aatagcacac acccacaaca acaacaaagg aaaactaaaa ggttttttct 
     4261 ttttgaaaag atcgttttct ttattattct ctagttttga cgctcgacat tttatgatgg 
     4321 aatgaatggg atgaatcatc aaacaagaga aaatacccgt gacgaaaata ataaaataag 
     4381 ttcctctgat acagaagatg aaaacaacaa caacaagata tagaaatgcc ttgggtggct 
     4441 attttatagt cttaactttt taatgtatat ttgttttgtt tttttacata ataatacttt 
     4501 ataaaagcta agctaaattc aagtaaaatt tcaatctctc aaataaaaca tttttctctt 
     4561 tttcttaaat ttagttttat atatttataa aataaacaaa gattttttta aaaaagtaac 
     4621 aagttatata tgtaataaca aaaagaagaa taacaagaat acaaaaccag atttccagat 
     4681 ttccagaatt tagatccatg ggtttgagag ttgtcgctat tgacggtggt gacgaaaaag 
     4741 gtgaatttgt taaatcattg ggtgctgaac caaactggat aatgataact gaagagaaga 
     4801 attagttttt tcaagtgtat aatatagttt ctctctatta ccttttccaa taatagcatt 
     4861 ttaagttttc tattttattt tgtataaaaa acataatgaa aaatacgtat aagtaatata 
     4921 aatgagtgtg ggattaagtg aatacgagat gttgtagtga taatagggga aactctttgg 
     4981 cgaaactaca agagagagtg atgtgctaat aatgaacgaa gaaatatgtg atttttgtat 
     5041 gaaatttgca attattctga ttgaatttgg gtacttgaca ttgaatccag aacgactata 
     5101 caaatgtgct actttgtcaa aatatccttt ttgagaatcg gcatatttat ggccctgaat 
     5161 atcgactacc acattccttt tacaacacta cgtaaccttt tgagaaagta caagtgaaag 
     5221 aagtatagaa ttcagtgttt agtttaacgt aagtattact gtggaatgct ttcttcgctc 
     5281 aagattgtcg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt 
     5341 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     5401 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     5461 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     5521 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     5581 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     5641 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     5701 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     5761 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     5821 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     5881 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     5941 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     6001 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     6061 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     6121 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     6181 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     6241 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     6301 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     6361 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     6421 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     6481 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     6541 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     6601 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6661 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6721 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     6781 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6841 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     6901 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     6961 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     7021 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     7081 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     7141 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     7201 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     7261 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     7321 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     7381 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     7441 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     7501 ac 
//
