LOCUS       pSpCas9(BB)-2A-GFP-hCYLD-Ex7        9290 bp    DNA     circular     21-FEB-2018
DEFINITION  LMBP 10536.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10536
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12170308|
COMMENT     VNTDBDATE|-12170308|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSpCas9(BB)-2A-GFP-hCYLD-Ex7|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT            (CStringList) 1 1 1 1 "1..247") 
COMMENT        #2=(CFSignal (CObList) "FLAG epitope" 22 0 0 1297 1320 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1297..1320") 
COMMENT        #3=(CFSignal (CObList) "SV40 large T NLS" 21 0 0 1327 1347 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1327..1347") 
COMMENT        #4=(CFSignal (CObList) "EGFP" 4 0 0 5587 6306 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5587..6306") 
COMMENT        #5=(CFSignal (CObList) "BGH polyA" 43 0 0 6337 6561 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6337..6561") 
COMMENT        #6=(CFSignal (CObList) "M13 ori" 33 0 0 6768 7223 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6768..7223") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 0 7610 8470 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "7610..8470") 
COMMENT        #8=(CFSignal (CObList) "AG promoter" 29 0 0 726 1142 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "726..1142") 
COMMENT        #9=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 442 725 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "hCas9" 4 0 0 1373 5473 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage 'pSpCas9(BB)-2A-GFP-hCYLD-Ex7'" 1) 
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COMMENT                          "LMBP 10536." 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 02/21/18 12:43" 1) 
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COMMENT                          "Last Modified: 02/21/18 12:43" 1) 
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COMMENT                          "length: 9290 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 9-AUG-2017" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     rep_origin      9011..9290
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        1..247
                     /vntifkey="29"
                     /label=hU6\promoter
     misc_marker     1297..1320
                     /vntifkey="22"
                     /label=FLAG\epitope
     misc_feature    1327..1347
                     /vntifkey="21"
                     /label=SV40\large\T\NLS
     CDS             5587..6306
                     /vntifkey="4"
                     /label=EGFP
     terminator      6337..6561
                     /vntifkey="43"
                     /label=BGH\polyA
     rep_origin      6768..7223
                     /vntifkey="33"
                     /label=M13\ori
     CDS             7610..8470
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        726..1142
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        442..725
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     CDS             1373..5473
                     /vntifkey="4"
                     /label=hCas9
     misc_feature    5528..5590
                     /vntifkey="21"
                     /label=T2A
     misc_feature    250..269
                     /vntifkey="21"
                     /label=hCYLD-Ex7\gRNA
BASE COUNT     2411 a      2491 c      2534 g      1854 t 
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag 
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga 
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat 
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga 
      241 cgaaacaccg agaagttcgt ctcggtctag ttttagagct agaaatagca agttaaaata 
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag 
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag 
      421 acaaatggct ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc 
      481 gcccaacgac ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg 
      541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
      601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca 
      661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
      721 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
      781 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
      841 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
      901 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
      961 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg 
     1021 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     1081 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 
     1141 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta 
     1201 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat 
     1261 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag 
     1321 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag 
     1381 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag 
     1441 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 
     1501 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 
     1561 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 
     1621 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 
     1681 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 
     1741 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 
     1801 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 
     1861 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 
     1921 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 
     1981 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 
     2041 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 
     2101 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 
     2161 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 
     2221 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 
     2281 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 
     2341 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 
     2401 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 
     2461 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 
     2521 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 
     2581 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 
     2641 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 
     2701 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 
     2761 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 
     2821 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 
     2881 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 
     2941 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 
     3001 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 
     3061 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 
     3121 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 
     3181 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 
     3241 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 
     3301 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 
     3361 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 
     3421 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 
     3481 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 
     3541 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 
     3601 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 
     3661 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 
     3721 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 
     3781 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 
     3841 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 
     3901 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 
     3961 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 
     4021 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 
     4081 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 
     4141 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 
     4201 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 
     4261 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 
     4321 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 
     4381 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 
     4441 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 
     4501 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 
     4561 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 
     4621 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 
     4681 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 
     4741 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 
     4801 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 
     4861 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 
     4921 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 
     4981 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 
     5041 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 
     5101 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac 
     5161 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac 
     5221 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag 
     5281 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc 
     5341 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac 
     5401 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag 
     5461 ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag 
     5521 gaattcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat 
     5581 cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 
     5641 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 
     5701 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 
     5761 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 
     5821 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 
     5881 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 
     5941 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 
     6001 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 
     6061 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 
     6121 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 
     6181 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 
     6241 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 
     6301 aaggaattct aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat 
     6361 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc 
     6421 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg 
     6481 ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg 
     6541 gggagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct 
     6601 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct 
     6661 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta 
     6721 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag 
     6781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     6841 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     6901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     6961 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata 
     7021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     7081 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc 
     7141 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 
     7201 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc 
     7261 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     7321 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     7381 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt 
     7441 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa 
     7501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     7561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     7621 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     7681 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 
     7741 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 
     7801 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc 
     7861 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 
     7921 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 
     7981 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 
     8041 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 
     8101 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 
     8161 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 
     8221 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 
     8281 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat 
     8341 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 
     8401 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 
     8461 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 
     8521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 
     8581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 
     8641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 
     8701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 
     8761 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 
     8821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 
     8881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 
     8941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 
     9001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 
     9061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 
     9121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 
     9181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 
     9241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 
//
