ID pKDsgRNA-FRT; SV 1; circular; unknown; STD; UNC; 6928 BP. DT 14-JUL.-2022 DE LMBP 11104. CC Accession number: LMBP 11104 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium). CC Last modified: 14-JUL-2022 FH Key Location/Qualifiers FH FT CDS complement(11..889) FT /label="araC" FT promoter 1163..1200 FT /label="araBAD promoter" FT CDS 1244..1660 FT /label="gam" FT CDS 1666..2451 FT /label="bet" FT CDS 2448..3128 FT /label="exo" FT terminator 3129..3373 FT /label="lambda tL3 terminator" FT CDS complement(3496..4446) FT /label="pSC101 repA(Ts)" FT mutation complement(4279..4281) FT /note="WT: GCT" FT /label="A56V" FT rep_origin 4449..4989 FT /label="pSC101 ori" FT promoter 5282..5335 FT /label="PLtetO-1 promoter" FT misc_feature 5336..5431 FT /label="sgRNA-FRT" FT misc_feature 5452..5481 FT /label="Myc epitope" FT misc_feature 5497..5514 FT /label="His-tag" FT terminator complement(5743..5786) FT /label="T1 terminator" FT CDS complement(5925..6716) FT /label="strepto-/spectinomycin resistance" FT RBS complement(6717..6751) FT /label="5' UTR of amp, incl. RBS" FT promoter complement(6833..6928) FT /label="ampicillin resistance promoter" SQ SEQUENCE 6928 AA; 2139224 MW; CATCGATTTA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC 60 GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT 120 CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA 180 GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT 240 GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA 300 TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT 360 TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT 420 CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA 480 TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG 540 TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT 600 AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC 660 CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA 720 CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT 780 CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG 840 CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC 900 TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG 960 TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT 1020 AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA 1080 GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT 1140 CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT 1200 ACCCGTTTTT TTGGGAATTC GAGCTCTAAG GAGGTTATAA AAAATGGATA TTAATACTGA 1260 AACTGAGATC AAGCAAAAGC ATTCACTAAC CCCCTTTCCT GTTTTCCTAA TCAGCCCGGC 1320 ATTTCGCGGG CGATATTTTC ACAGCTATTT CAGGAGTTCA GCCATGAACG CTTATTACAT 1380 TCAGGATCGT CTTGAGGCTC AGAGCTGGGC GCGTCACTAC CAGCAGCTCG CCCGTGAAGA 1440 GAAAGAGGCA GAACTGGCAG ACGACATGGA AAAAGGCCTG CCCCAGCACC TGTTTGAATC 1500 GCTATGCATC GATCATTTGC AACGCCACGG GGCCAGCAAA AAATCCATTA CCCGTGCGTT 1560 TGATGACGAT GTTGAGTTTC AGGAGCGCAT GGCAGAACAC ATCCGGTACA TGGTTGAAAC 1620 CATTGCTCAC CACCAGGTTG ATATTGATTC AGAGGTATAA AACGAATGAG TACTGCACTC 1680 GCAACGCTGG CTGGGAAGCT GGCTGAACGT GTCGGCATGG ATTCTGTCGA CCCACAGGAA 1740 CTGATCACCA CTCTTCGCCA GACGGCATTT AAAGGTGATG CCAGCGATGC GCAGTTCATC 1800 GCATTACTGA TCGTTGCCAA CCAGTACGGC CTTAATCCGT GGACGAAAGA AATTTACGCC 1860 TTTCCTGATA AGCAGAATGG CATCGTTCCG GTGGTGGGCG TTGATGGCTG GTCCCGCATC 1920 ATCAATGAAA ACCAGCAGTT TGATGGCATG GACTTTGAGC AGGACAATGA ATCCTGTACA 1980 TGCCGGATTT ACCGCAAGGA CCGTAATCAT CCGATCTGCG TTACCGAATG GATGGATGAA 2040 TGCCGCCGCG AACCATTCAA AACTCGCGAA GGCAGAGAAA TCACGGGGCC GTGGCAGTCG 2100 CATCCCAAAC GGATGTTACG TCATAAAGCC ATGATTCAGT GTGCCCGTCT GGCCTTCGGA 2160 TTTGCTGGTA TCTATGACAA GGATGAAGCC GAGCGCATTG TCGAAAATAC TGCATACACT 2220 GCAGAACGTC AGCCGGAACG CGACATCACT CCGGTTAACG ATGAAACCAT GCAGGAGATT 2280 AACACTCTGC TGATCGCCCT GGATAAAACA TGGGATGACG ACTTATTGCC GCTCTGTTCC 2340 CAGATATTTC GCCGCGACAT TCGTGCATCG TCAGAACTGA CACAGGCCGA AGCAGTAAAA 2400 GCTCTTGGAT TCCTGAAACA GAAAGCCGCA GAGCAGAAGG TGGCAGCATG ACACCGGACA 2460 TTATCCTGCA GCGTACCGGG ATCGATGTGA GAGCTGTCGA ACAGGGGGAT GATGCGTGGC 2520 ACAAATTACG GCTCGGCGTC ATCACCGCTT CAGAAGTTCA CAACGTGATA GCAAAACCCC 2580 GCTCCGGAAA GAAGTGGCCT GACATGAAAA TGTCCTACTT CCACACCCTG CTTGCTGAGG 2640 TTTGCACCGG TGTGGCTCCG GAAGTTAACG CTAAAGCACT GGCCTGGGGA AAACAGTACG 2700 AGAACGACGC CAGAACCCTG TTTGAATTCA CTTCCGGCGT GAATGTTACT GAATCCCCGA 2760 TCATCTATCG CGACGAAAGT ATGCGTACCG CCTGCTCTCC CGATGGTTTA TGCAGTGACG 2820 GCAACGGCCT TGAACTGAAA TGCCCGTTTA CCTCCCGGGA TTTCATGAAG TTCCGGCTCG 2880 GTGGTTTCGA GGCCATAAAG TCAGCTTACA TGGCCCAGGT GCAGTACAGC ATGTGGGTGA 2940 CGCGAAAAAA TGCCTGGTAC TTTGCCAACT ATGACCCGCG TATGAAGCGT GAAGGCCTGC 3000 ATTATGTCGT GATTGAGCGG GATGAAAAGT ACATGGCGAG TTTTGACGAG ATCGTGCCGG 3060 AGTTCATCGA AAAAATGGAC GAGGCACTGG CTGAAATTGG TTTTGTATTT GGGGAGCAAT 3120 GGCGATGACG CATCCTCACG ATAATATCCG GGTAGGCGCA ATCACTTTCG TCTACTCCGT 3180 TACAAAGCGA GGCTGGGTAT TTCCCGGCCT TTCTGTTATC CGAAATCCAC TGAAAGCACA 3240 GCGGCTGGCT GAGGAGATAA ATAATAAACG AGGGGCTGTA TGCACAAAGC ATCTTCTGTT 3300 GAGTTAAGAA CGAGTATCGA GATGGCACAT AGCCTTGCTC AAATTGGAAT CAGGTTTGTG 3360 CCAATACCAG TAGAAACAGA CGAAGAATCC ATGGGTATGG ACAGTTTTCC CTTTGATATG 3420 TAACGGTGAA CAGTTGTTCT ACTTTTGTTT GTTAGTCTTG ATGCTTCACT GATAGATACA 3480 AGAGCCATAA GAACCTCAGA TCCTTCCGTA TTTAGCCAGT ATGTTCTCTA GTGTGGTTCG 3540 TTGTTTTTGC GTGAGCCATG AGAACGAACC ATTGAGATCA TACTTACTTT GCATGTCACT 3600 CAAAAATTTT GCCTCAAAAC TGGTGAGCTG AATTTTTGCA GTTAAAGCAT CGTGTAGTGT 3660 TTTTCTTAGT CCGTTACGTA GGTAGGAATC TGATGTAATG GTTGTTGGTA TTTTGTCACC 3720 ATTCATTTTT ATCTGGTTGT TCTCAAGTTC GGTTACGAGA TCCATTTGTC TATCTAGTTC 3780 AACTTGGAAA ATCAACGTAT CAGTCGGGCG GCCTCGCTTA TCAACCACCA ATTTCATATT 3840 GCTGTAAGTG TTTAAATCTT TACTTATTGG TTTCAAAACC CATTGGTTAA GCCTTTTAAA 3900 CTCATGGTAG TTATTTTCAA GCATTAACAT GAACTTAAAT TCATCAAGGC TAATCTCTAT 3960 ATTTGCCTTG TGAGTTTTCT TTTGTGTTAG TTCTTTTAAT AACCACTCAT AAATCCTCAT 4020 AGAGTATTTG TTTTCAAAAG ACTTAACATG TTCCAGATTA TATTTTATGA ATTTTTTTAA 4080 CTGGAAAAGA TAAGGCAATA TCTCTTCACT AAAAACTAAT TCTAATTTTT CGCTTGAGAA 4140 CTTGGCATAG TTTGTCCACT GGAAAATCTC AAAGCCTTTA ACCAAAGGAT TCCTGATTTC 4200 CACAGTTCTC GTCATCAGCT CTCTGGTTGC TTTAGCTAAT ACACCATAAG CATTTTCCCT 4260 ACTGATGTTC ATCATCTGAA CGTATTGGTT ATAAGTGAAC GATACCGTCC GTTCTTTCCT 4320 TGTAGGGTTT TCAATCGTGG GGTTGAGTAG TGCCACACAG CATAAAATTA GCTTGGTTTC 4380 ATGCTCCGTT AAGTCATAGC GACTAATCGC TAGTTCATTT GCTTTGAAAA CAACTAATTC 4440 AGACATACAT CTCAATTGGT CTAGGTGATT TTAATCACTA TACCAATTGA GATGGGCTAG 4500 TCAATGATAA TTACTAGTCC TTTTCCTTTG AGTTGTGGGT ATCTGTAAAT TCTGCTAGAC 4560 CTTTGCTGGA AAACTTGTAA ATTCTGCTAG ACCCTCTGTA AATTCCGCTA GACCTTTGTG 4620 TGTTTTTTTT GTTTATATTC AAGTGGTTAT AATTTATAGA ATAAAGAAAG AATAAAAAAA 4680 GATAAAAAGA ATAGATCCCA GCCCTGTGTA TAACTCACTA CTTTAGTCAG TTCCGCAGTA 4740 TTACAAAAGG ATGTCGCAAA CGCTGTTTGC TCCTCTACAA AACAGACCTT AAAACCCTAA 4800 AGGCTTAAGT AGCACCCTCG CAAGCTCGGT TGCGGCCGCA ATCGGGCAAA TCGCTGAATA 4860 TTCCTTTTGT CTCCGACCAT CAGGCACCTG AGTCGCTGTC TTTTTCGTGA CATTCAGTTC 4920 GCTGCGCTCA CGGCTCTGGC AGTGAATGGG GGTAAATGGC ACTACAGGCG CCTTTTATGG 4980 ATTCATGCAA GGAAACTACC CATAATACAA GAAAAGCCCG TCACGGGCTT CTCAGGGCGT 5040 TTTATGGCGG GTCTGCTATG TGGTGCTATC TGACTTTTTG CTGTTCAGCA GTTCCTGCCC 5100 TCTGATTTTC CAGTCTGACC ACTTCGGATT ATCCCGTGAC AGGTCATTCA GACTGGCTAA 5160 TGCACCCAGT AAGGCAGCGG TATCATCAAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 5220 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AGCTTTCGCT AAGGATGATT TCTGGAATTC 5280 TTCCCTATCA GTGATAGAGA TTGACATCCC TATCAGTGAT AGAGATACTG AGCACTCCTA 5340 TACTTTCTAG AGAATGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA 5400 TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGA AGCTTGGGCC CGAACAAAAA 5460 CTCATCTCAG AAGAGGATCT GAATAGCGCC GTCGACCATC ATCATCATCA TCATTGAGTT 5520 TAAACGGTCT CCAGCTTGGC TGTTTTGGCG GATGAGAGAA GATTTTCAGC CTGATACAGA 5580 TTAAATCAGA ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG 5640 TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG 5700 TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG 5760 TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACTGGAT CCTTACTCGA 5820 GTCTAGACTG CAGGCGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 5880 CAATCTAAAG TATATATGAG TAAACTTGGT CTGCTGACAG GACATTATTT GCCGACTACC 5940 TTGGTGATCT CGCCTTTCAC GTAGTGGACA AATTCTTCCA ACTGATCTGC GCGCGAGGCC 6000 AAGCGATCTT CTTCTTGTCC AAGATAAGCC TGTCTAGCTT CAAGTATGAC GGGCTGATAC 6060 TGGGCCGGCA GGCGCTCCAT TGCCCAGTCG GCAGCGACAT CCTTCGGCGC GATTTTGCCG 6120 GTTACTGCGC TGTACCAAAT GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC 6180 CAGTCGGGCG GCGAGTTCCA TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT 6240 TCAGGAACCG GATCAAAGAG TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT 6300 CTTGCTTTTG TCAGCAAGAT AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT 6360 GCAAGAATGT CATTGCGCTG CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC 6420 CACGGAATGA TGTCGTCGTG CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC 6480 TCTCCAGGGG AAGCCGAAGT TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA 6540 TCAAGCCTTA CGGTCACCGT AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA 6600 TCCACTGCGG AGCCGTACAA ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG 6660 ACGCCAACTA CCTCTGATAG TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATACTC 6720 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 6780 TTTGAATGTA TTTAGAAAAA TAAACAAATA GCTAGCTCAC TCGGTCGCTA CCAGGGTTAT 6840 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 6900 CGCACATTTC CCCGAAAAGT GCCACCTG 6928 //