LOCUS       pTetO7TATA-dCas9-Mxi1-HIS3-CNE1gRNA3      9536 bp    DNA     circular UNA 22-Jun-2015
DEFINITION  LMBP 11110.
FEATURES             Location/Qualifiers
     CDS             63..923
                     /label=AmpR
     promoter        complement(965..993)
                     /label="AmpR promoter"
     rep_origin      1055..1582
                     /label="CEN6 ARSH4"
     primer_bind     1615..1643
                     /label="A250_F part homology"
     promoter        1666..1982
                     /label="HIS3 promoter"
     CDS             1983..2642
                     /label=HIS3
     terminator      2643..2843
                     /label="HIS3 TT"
     primer_bind     complement(2856..2885)
                     /label="A250_R part homology"
     sequenced       complement(2856..>2859)
     misc_feature    2888
                     /feature_type=Misc.
                     /note="From here till end correctly sequenced"
                     /label="Seq R"
     primer_bind     complement(2934..2952)
                     /note="primer walking primer"
                     /label=A068_R
     Operator        2934..3227
                     /label=TetO7
     primer_bind     3248..3267
                     /label=A072_F
     primer_bind     complement(3386..3403)
                     /label=A224_R_partCas9
     primer_bind     complement(3386..3435)
                     /label=A245_R
     primer_bind     complement(3387..3404)
                     /note="starts with CAT (ATG codon), and before that
                     homology region"
                     /label=RP188_down
     sequenced       <3405..3567
     CDS             3406..7605
                     /label=dCas9
     sequenced       complement(<3409..3560)
     CDS             3415..3441
                     /label="NLS+GS linker"
     sequenced       complement(5455..5524)
                     /label="sequence OK"
     sequenced       complement(5525..5594)
                     /label="sequence OK"
     sequenced       complement(5595..5664)
                     /label="sequence OK"
     sequenced       complement(5665..5734)
                     /label="sequence OK"
     sequenced       complement(5735..5804)
                     /label="sequence OK"
     sequenced       complement(5805..5874)
                     /label="sequence OK"
     sequenced       complement(5875..5944)
                     /label="sequence OK"
     sequenced       complement(5945..6014)
                     /label="sequence OK"
     sequenced       complement(6015..6084)
                     /label="sequence OK"
     sequenced       complement(6085..6154)
                     /label="sequence OK"
     sequenced       complement(6155..6224)
                     /label="sequence OK"
     mutation        complement(6231)
                     /label="poss T"
     sequenced       complement(6232..6294)
                     /label="sequence OK"
     sequenced       complement(6888..7779)
                     /label="sequence OK"
     primer_bind     7375..7393
                     /label=A232_F
     CDS             7606..7734
                     /label="Mxi1 domain"
     terminator      7741..7952
                     /label="ADH1 3UTR and TT"
     primer_bind     complement(7886..7903)
                     /label=A232_R
     terminator      complement(7971..7989)
                     /label="SUP4 TT"
     misc_RNA        complement(7990..8033)
                     /label="S. pyogenes terminator"
     misc_RNA        complement(8034..8075)
                     /label="dCas9 handle"
     misc_RNA        complement(8076..8095)
                     /label="CNE1 gRNA3"
     misc_RNA        complement(8096)
                     /label="splice site"
     misc_RNA        complement(8097..8254)
                     /label="SNR52 preplice"
     promoter        complement(8255..8364)
                     /label="SNR52 promoter"
     rep_origin      complement(8826..9444)
                     /label="pBR322 ori"
ORIGIN
        1 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
       61 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      121 atagttgcct gactgcccgt cgtgtagata actacgatac gggagggctt accatctggc
      181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
      241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
      301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
      361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
      421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgaaaaaaa
      481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
      541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
      601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
      661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
      721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
      781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1021 gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa
     1081 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     1141 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     1201 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     1261 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     1321 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     1381 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     1441 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     1501 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     1561 aagcgatccg tcctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1621 cacgaggccc tttcgtctcg cgcgTTGTAC TGAGAGTGCA CCATAAATTC CCGTTTTAAG
     1681 AGCTTGGTGA GCGCTAGGAG TCACTGCCAG GTATCGTTTG AACACGGCAT TAGTCAGGGA
     1741 AGTCATAACA CAGTCCTTTC CCGCAATTTT CTTTTTCTAT TACTCTTGGC CTCCTCTAGT
     1801 ACACTCTATA TTTTTTTATG CCTCGGTAAT GATTTTCATT TTTTTTTTTC CCCTAGCGGA
     1861 TGACTCTTTT TTTTTCTTAG CGATTGGCAT TATCACATAA TGAATTATAC ATTATATAAA
     1921 GTAATGTGAT TTCTTCGAAG AATATACTAA AAAATGAGCA GGCAAGATAA ACGAAGGCAA
     1981 AGATGACAGA GCAGAAAGCC CTAGTAAAGC GTATTACAAA TGAAACCAAG ATTCAGATTG
     2041 CGATCTCTTT AAAGGGTGGT CCCCTAGCGA TAGAGCACTC GATCTTCCCA GAAAAAGAGG
     2101 CAGAAGCAGT AGCAGAACAG GCCACACAAT CGCAAGTGAT TAACGTCCAC ACAGGTATAG
     2161 GGTTTCTGGA CCATATGATA CATGCTCTGG CCAAGCATTC CGGCTGGTCG CTAATCGTTG
     2221 AGTGCATTGG TGACTTACAC ATAGACGACC ATCACACCAC TGAAGACTGC GGGATTGCTC
     2281 TCGGTCAAGC TTTTAAAGAG GCCCTACTGG CGCGTGGAGT AAAAAGGTTT GGATCAGGAT
     2341 TTGCGCCTTT GGATGAGGCA CTTTCCAGAG CGGTGGTAGA TCTTTCGAAC AGGCCGTACG
     2401 CAGTTGTCGA ACTTGGTTTG CAAAGGGAGA AAGTAGGAGA TCTCTCTTGC GAGATGATCC
     2461 CGCATTTTCT TGAAAGCTTT GCAGAGGCTA GCAGAATTAC CCTCCACGTT GATTGTCTGC
     2521 GAGGCAAGAA TGATCATCAC CGTAGTGAGA GTGCGTTCAA GGCTCTTGCG GTTGCCATAA
     2581 GAGAAGCCAC CTCGCCCAAT GGTACCAACG ATGTTCCCTC CACCAAAGGT GTTCTTATGT
     2641 AGTGACACCG ATTATTTAAA GCTGCAGCAT ACGATATATA TACATGTGTA TATATGTATA
     2701 CCTATGAATG TCAGTAAGTA TGTATACGAA CAGTATGATA CTGAAGATGA CAAGGTAATG
     2761 CATCATTCTA TACGTGTCAT TCTGAACGAG GCGCGCTTTC CTTTTTTCTT TTTGCTTTTT
     2821 CTTTTTTTTT CTCTTGAACT CGACGGATCT ATGCGAATTC GCTCTTATTG ACCACACCTC
     2881 TACCGGCAGA TCCGCTAGGG ATAACAGGGT AATATAGATC AATTCCTCGA TCGAGTTTAC
     2941 CACTCCCTAT CAGTGATAGA GAAAAGTGAA AGTCGAGTTT ACCACTCCCT ATCAGTGATA
     3001 GAGAAAAGTG AAAGTCGAGT TTACCACTCC CTATCAGTGA TAGAGAAAAG TGAAAGTCGA
     3061 GTTTACCACT CCCTATCAGT GATAGAGAAA AGTGAAAGTC GAGTTTACCA CTCCCTATCA
     3121 GTGATAGAGA AAAGTGAAAG TCGAGTTTAC CACTCCCTAT CAGTGATAGA GAAAAGTGAA
     3181 AGTCGAGTTT ACCACTCCCT ATCAGTGATA GAGAAAAGTG AAAGTCGAGC TCGGTACCCT
     3241 ATGGCATGCA TGTGCTCTGT ATGTATATAA AACTCTTGTT TTCTTCTTTT CTCTAAATAT
     3301 TCTTTCCTTA TACATTAGGT CCTTTGTAGC ATAAATTACT ATACTTCTAT AGACACGCAA
     3361 ACACAAATAC ACACACTAAA TTACCGGATC AATTCGGGGG ATCCCATGGA CGTCCCAAAG
     3421 AAGAAGAGAA AGGTAGGCAG CGACAAGAAG TATTCTATCG GACTGGCCAT CGGGACTAAT
     3481 AGCGTCGGGT GGGCCGTGAT CACTGACGAG TACAAGGTGC CCTCTAAGAA GTTCAAGGTG
     3541 CTCGGGAACA CCGACCGGCA TTCCATCAAG AAAAATCTGA TCGGAGCTCT CCTCTTTGAT
     3601 TCAGGGGAGA CCGCTGAAGC AACCCGCCTC AAGCGGACTG CTAGACGGCG GTACACCAGG
     3661 AGGAAGAACC GGATTTGTTA CCTTCAAGAG ATATTCTCCA ACGAAATGGC AAAGGTCGAC
     3721 GACAGCTTCT TCCATAGGCT GGAAGAATCA TTCCTCGTGG AAGAGGATAA GAAGCATGAA
     3781 CGGCATCCCA TCTTCGGTAA TATCGTCGAC GAGGTGGCCT ATCACGAGAA ATACCCAACC
     3841 ATCTACCATC TTCGCAAAAA GCTGGTGGAC TCAACCGACA AGGCAGACCT CCGGCTTATC
     3901 TACCTGGCCC TGGCCCACAT GATCAAGTTC AGAGGCCACT TCCTGATCGA GGGCGACCTC
     3961 AATCCTGACA ATAGCGATGT GGATAAACTG TTCATCCAGC TGGTGCAGAC TTACAACCAG
     4021 CTCTTTGAAG AGAACCCCAT CAATGCAAGC GGAGTCGATG CCAAGGCCAT TCTGTCAGCC
     4081 CGGCTGTCAA AGAGCCGCAG ACTTGAGAAT CTTATCGCTC AGCTGCCGGG TGAAAAGAAA
     4141 AATGGACTGT TCGGGAACCT GATTGCTCTT TCACTTGGGC TGACTCCCAA TTTCAAGTCT
     4201 AATTTCGACC TGGCAGAGGA TGCCAAGCTG CAACTGTCCA AGGACACCTA TGATGACGAT
     4261 CTCGACAACC TCCTGGCCCA GATCGGTGAC CAATACGCCG ACCTTTTCCT TGCTGCTAAG
     4321 AATCTTTCTG ACGCCATCCT GCTGTCTGAC ATTCTCCGCG TGAACACTGA AATCACCAAG
     4381 GCCCCTCTTT CAGCTTCAAT GATTAAGCGG TATGATGAGC ACCACCAGGA CCTGACCCTG
     4441 CTTAAGGCAC TCGTCCGGCA GCAGCTTCCG GAGAAGTACA AGGAAATCTT CTTTGACCAG
     4501 TCAAAGAATG GATACGCCGG CTACATCGAC GGAGGTGCCT CCCAAGAGGA ATTTTATAAG
     4561 TTTATCAAAC CTATCCTTGA GAAGATGGAC GGCACCGAAG AGCTCCTCGT GAAACTGAAT
     4621 CGGGAGGATC TGCTGCGGAA GCAGCGCACT TTCGACAATG GGAGCATTCC CCACCAGATC
     4681 CATCTTGGGG AGCTTCACGC CATCCTTCGG CGCCAAGAGG ACTTCTACCC CTTTCTTAAG
     4741 GACAACAGGG AGAAGATTGA GAAAATTCTC ACTTTCCGCA TCCCCTACTA CGTGGGACCC
     4801 CTCGCCAGAG GAAATAGCCG GTTTGCTTGG ATGACCAGAA AGTCAGAAGA AACTATCACT
     4861 CCCTGGAACT TCGAAGAGGT GGTGGACAAG GGAGCCAGCG CTCAGTCATT CATCGAACGG
     4921 ATGACTAACT TCGATAAGAA CCTCCCCAAT GAGAAGGTCC TGCCGAAACA TTCCCTGCTC
     4981 TACGAGTACT TTACCGTGTA CAACGAGCTG ACCAAGGTGA AATATGTCAC CGAAGGGATG
     5041 AGGAAGCCCG CATTCCTGTC AGGCGAACAA AAGAAGGCAA TTGTGGACCT TCTGTTCAAG
     5101 ACCAATAGAA AGGTGACCGT GAAGCAGCTG AAGGAGGACT ATTTCAAGAA AATTGAATGC
     5161 TTCGACTCTG TGGAGATTAG CGGGGTCGAA GATCGGTTCA ACGCAAGCCT GGGTACCTAC
     5221 CATGATCTGC TTAAGATCAT CAAGGACAAG GATTTTCTGG ACAATGAGGA GAACGAGGAC
     5281 ATCCTTGAGG ACATTGTCCT GACTCTCACT CTGTTCGAGG ACCGGGAAAT GATCGAGGAG
     5341 AGGCTTAAGA CCTACGCCCA TCTGTTCGAC GATAAAGTGA TGAAGCAACT TAAACGGAGA
     5401 AGATATACCG GATGGGGACG CCTTAGCCGC AAACTCATCA ACGGAATCCG GGACAAACAG
     5461 AGCGGAAAGA CCATTCTTGA TTTCCTTAAG AGCGACGGAT TCGCTAATCG CAACTTCATG
     5521 CAACTTATCC ATGATGATTC CCTGACCTTT AAGGAGGACA TCCAGAAGGC CCAAGTGTCT
     5581 GGACAAGGTG ACTCACTGCA CGAGCATATC GCAAATCTGG CTGGTTCACC CGCTATTAAG
     5641 AAGGGTATTC TCCAGACCGT GAAAGTCGTG GACGAGCTGG TCAAGGTGAT GGGTCGCCAT
     5701 AAACCAGAGA ACATTGTCAT CGAGATGGCC AGGGAAAACC AGACTACCCA GAAGGGACAG
     5761 AAGAACAGCA GGGAGCGGAT GAAAAGAATT GAGGAAGGGA TTAAGGAGCT CGGGTCACAG
     5821 ATCCTTAAAG AGCACCCGGT GGAAAACACC CAGCTTCAGA ATGAGAAGCT CTATCTGTAC
     5881 TACCTTCAAA ATGGACGCGA TATGTATGTG GACCAAGAGC TTGATATCAA CAGGCTCTCA
     5941 GACTACGACG TGGACGCCAT CGTCCCTCAG AGCTTCCTCA AAGACGACTC AATTGACAAT
     6001 AAGGTGCTGA CTCGCTCAGA CAAGAACCGG GGAAAGTCAG ATAACGTGCC CTCAGAGGAA
     6061 GTCGTGAAAA AGATGAAGAA CTATTGGCGC CAGCTTCTGA ACGCAAAGCT GATCACTCAG
     6121 CGGAAGTTCG ACAATCTCAC TAAGGCTGAG AGGGGCGGAC TGAGCGAACT GGACAAAGCA
     6181 GGATTCATTA AACGGCAACT TGTGGAGACT CGGCAGATTA CTAAACATGT CGCCCAAATC
     6241 CTTGACTCAC GCATGAATAC CAAGTACGAC GAAAACGACA AACTTATCCG CGAGGTGAAG
     6301 GTGATTACCC TGAAGTCCAA GCTGGTCAGC GATTTCAGAA AGGACTTTCA ATTCTACAAA
     6361 GTGCGGGAGA TCAATAACTA TCATCATGCT CATGACGCAT ATCTGAATGC CGTGGTGGGA
     6421 ACCGCCCTGA TCAAGAAGTA CCCAAAGCTG GAAAGCGAGT TCGTGTACGG AGACTACAAG
     6481 GTCTACGACG TGCGCAAGAT GATTGCCAAA TCTGAGCAGG AGATCGGAAA GGCCACCGCA
     6541 AAGTACTTCT TCTACAGCAA CATCATGAAT TTCTTCAAGA CCGAAATCAC CCTTGCAAAC
     6601 GGTGAGATCC GGAAGAGGCC GCTCATCGAG ACTAATGGGG AGACTGGCGA AATCGTGTGG
     6661 GACAAGGGCA GAGATTTCGC TACCGTGCGC AAAGTGCTTT CTATGCCTCA AGTGAACATC
     6721 GTGAAGAAAA CCGAGGTGCA AACCGGAGGC TTTTCTAAGG AATCAATCCT CCCCAAGCGC
     6781 AACTCCGACA AGCTCATTGC AAGGAAGAAG GATTGGGACC CTAAGAAGTA CGGCGGATTC
     6841 GATTCACCAA CTGTGGCTTA TTCTGTCCTG GTCGTGGCTA AGGTGGAAAA AGGAAAGTCT
     6901 AAGAAGCTCA AGAGCGTGAA GGAACTGCTG GGTATCACCA TTATGGAGCG CAGCTCCTTC
     6961 GAGAAGAACC CAATTGACTT TCTCGAAGCC AAAGGTTACA AGGAAGTCAA GAAGGACCTT
     7021 ATCATCAAGC TCCCAAAGTA TAGCCTGTTC GAACTGGAGA ATGGGCGGAA GCGGATGCTC
     7081 GCCTCCGCTG GCGAACTTCA GAAGGGTAAT GAGCTGGCTC TCCCCTCCAA GTACGTGAAT
     7141 TTCCTCTACC TTGCAAGCCA TTACGAGAAG CTGAAGGGGA GCCCCGAGGA CAACGAGCAA
     7201 AAGCAACTGT TTGTGGAGCA GCATAAGCAT TATCTGGACG AGATCATTGA GCAGATTTCC
     7261 GAGTTTTCTA AACGCGTCAT TCTCGCTGAT GCCAACCTCG ATAAAGTCCT TAGCGCATAC
     7321 AATAAGCACA GAGACAAACC AATTCGGGAG CAGGCTGAGA ATATCATCCA CCTGTTCACC
     7381 CTCACCAATC TTGGTGCCCC TGCCGCATTC AAGTACTTCG ACACCACCAT CGACCGGAAA
     7441 CGCTATACCT CCACCAAAGA AGTGCTGGAC GCCACCCTCA TCCACCAGAG CATCACCGGA
     7501 CTTTACGAAA CTCGGATTGA CCTCTCACAG CTCGGAGGGG ATGAGGGAGC TCCCAAGAAA
     7561 AAGCGCAAGG TAGGTAGTTC CAAGCTTGGC GGCAGCGGCG GCAGCATGGA ACGTGTGAGA
     7621 ATGATTAATG TGCAAAGGCT GTTAGAAGCC GCAGAGTTTT TAGAAAGAAG AGAAAGAGAA
     7681 TGCGAACACG GGTATGCCAG TTCTTTCCCT AGCATGCCCT CTCCCAGAGG CTAACTCGAG
     7741 GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA
     7801 TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT TCTTATTCTT GAGTAACTCT
     7861 TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG TCGCTCTTAT TGACCACACC
     7921 TCTACCGGCA TGCCGAGCAA ATGCCTGCAA ATCGCTCCCC ATTTCTAGGA CTGCAGAGAC
     7981 ATAAAAAACA AAAAAAGCAC CACCGACTCG GTGCCACTTT TTCAAGTTGA TAACGGACTA
     8041 GCCTTATTTT AACTTGCTAT TTCTAGCTCT AAAACTAAAC GACATGCATC ACACGGATCA
     8101 TTTATCTTTC ACTGCGGAGA AGTTTCGAAC GCCGAAACAT GCGCACCAAC TTTCACTTCT
     8161 ACAGCGTTTG ACCAAAATCT TTTGAACAGA ACATTGTAGG GTGTGAAAAA ATGCGCACCT
     8221 TTACCGCTAG CCCAAGAGGG CACTACAAAA TCTAGAGTTG TACTTCAAAC GTACATGTAA
     8281 TCACCTTGTA TATACTCGAA AGAAAACATC AAGTTTCTGT ATAAATATGA GTGAAAGCAT
     8341 AATCATACAT TATCTTTTCA AAGAAtaaga CTGCAGagtt cGGTACCagc ttttgttccc
     8401 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8461 attgttatcc gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct
     8521 ggggtgccta atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc
     8581 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8641 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8701 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8761 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8821 aggccgcgtt gctggcgttt ttccataggc tcggcccccc tgacgagcat cacaaaaatc
     8881 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgttccccc
     8941 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9001 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     9061 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9121 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9181 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9241 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9301 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9361 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9421 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9481 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atcctt
//

