LOCUS       pGAPNOR-MNN4C-cloneEZ      5807 bp    DNA     circular UNA 24-JAN-2012
DEFINITION  LMBP 11113.
ACCESSION   pGAPNOR-MNN4C-cloneEZ
FEATURES             Location/Qualifiers
     promoter        1..483
                     /label=GAP
     misc_feature    470..2776
                     /feature_type=Insertion
                     /note="Region on donor sequence (MNN4C-cloneEZ): 15..2321"
                     /label=MNN4C-cloneEZ
     CDS             484..2772
                     /label=MNN4C
     misc_feature    2777..>5807
                     /feature_type=Insertion
                     /note="Region on donor sequence
                     (pGAPNORMnn2SpGal10hGalTr): 4255..1927"
                     /label=pGAPNORMnn2SpGal10hGalTr
     terminator      2803..3143
                     /note="Oriinal location: 4281..4621"
                     /label="AOX1 TT"
     promoter        3144..3554
                     /note="Oriinal location: 4622..5032"
                     /label="P TEF1"
     CDS             3623..4195
                     /note="Oriinal location: 5101..5673"
                     /label="Nor R"
     terminator      4748..5065
                     /note="Oriinal location: 399..716"
                     /label="CYC1 TT"
     rep_origin      5076..5749
                     /note="Oriinal location: 727..1400"
                     /label=ColE1
ORIGIN
        1 AGATCTTTTT TGTAGAAATG TCTTGGTGTC CTCGTCCAAT CAGGTAGCCA TCTCTGAAAT
       61 ATCTGGCTCC GTTGCAACTC CGAACGACCT GCTGGCAACG TAAAATTCTC CGGGGTAAAA
      121 CTTAAATGTG GAGTAATGGA ACCAGAAACG TCTCTTCCCT TCTCTCTCCT TCCACCGCCC
      181 GTTACCGTCC CTAGGAAATT TTACTCTGCT GGAGAGCTTC TTCTACGGCC CCCTTGCAGC
      241 AATGCTCTTC CCAGCATTAC GTTGCGGGTA AAACGGAGGT CGTGTACCCG ACCTAGCAGC
      301 CCAGGGATGG AAAAGTCCCG GCCGTCGCTG GCAATAATAG CGGGCGGACG CATGTCATGA
      361 GATTATTGGA AACCACCAGA ATCGAATATA AAAGGCGAAC ACCTTTCCCA ATTTTGGTTT
      421 CTCCTGACCC AAAGACTTTA AATTTAATTT ATTTGTCCCT ATTTCAATCA ATTGAACAAC
      481 TATATGAGTG GCAATCCTTT TCTGTTCTCT CCTTCAAATT TTGACTTTTC TGGTTTGGAT
      541 CATTATAGAT CCACTGATAA AGATCACTTA GCTCTAGATG TTCTCGATTA TGACAAAAAT
      601 CACTTCTTCT CCAGAAACTC CCCCAGTTTG AAATCTCGTA TTCACTTTTA TCGACATAAA
      661 TTGACCACTA GAAAGCAAAT TGGACTTTTC AGCGGCAGAC TGAAGCTTTT TGTGCTTGCT
      721 CTCTTTGTGT TGATCACATT TTCTGCAATC CACATTCCAA TCCCTTTCTC TTTGGATATT
      781 CTAGGTTCCC ATGTCAAATA CCTGCCCTTA CGAGAGAAAG TCGATCCGGA AGAGGCACTC
      841 CATCTGCACG GACTGGATCT CTCGGTAGCA GAGCTACCTT TTTTCAATGA TGACATGATG
      901 TCTGAATTTA ACTACGATCC TAGACTACCC ACCGCTTTGA TTTTGAAGTT AGTGTTAGAT
      961 CATATAAGTG TGCGTAATGG AACGTTTGAT GCTAAGTTTA AGGTCCCCTT TAACTGGAAA
     1021 CTTTGGGTGG ATTTGCATTC AAGGTTAGTT CCATCTAATA GTTGGTATAA TCGATTTCGA
     1081 TTACCCTCAG GTCGTTTCGA AACATGCGAT GAATTTAAGA GGTTTTTCGG AATCACTAAG
     1141 AATCACTTTG GAACAGACCT TGATAATTGC GTTGATATCG AGTATGATAC TCCGGAAGGT
     1201 TATCCAAAGT TCAAAGTTTT GCATGCGGAA GATAAAGCTC TTCCTTATGA AGCACGTATC
     1261 ATTTATGGTG CTTCTTACCT TTACCACGAA GCACAGAATC CTAAAAGGTT GATATTTTTA
     1321 GGATTGGGCA AGTCCAATGA GTCTTTGATC TTACCAGTTG AGGCAAATGA CAGTTCCAAC
     1381 TTAATGCAAT TCAACCACGA ATATGCAAGA AGCTTTAACG ATCAACCTTT CGTTTCTCTT
     1441 GAGGAACTTG TCAAGAAGGT TTCACTGACC TTGAATTTGA ATAGTGATAA GGTGCTACCA
     1501 ATCAATGAAC TGGACGTTAT CAAAGACACC CCGCGCTTAA TGAATCACAA CAACCAGGGA
     1561 CTGAGCATAG ACAAGAGCTC ATTTCAATGG GATCTGGAAA GGGAATTACA GTTGTTAGAA
     1621 CATAGAACCA GTCAAGTTAA TGACGTGGAA GGCCTTGATG CGGGTATTTA TTCAACAATT
     1681 CAATGTGAAA TGCGCTCTAT GTACGATTTT TCAAAATACT TCCATGAATC AAAAGTCTCT
     1741 GGTAAATATC TTCCTTCTGG AGAGCACTAT GACTGGCGAT TTTTTAATGG TTTTTACCTT
     1801 TCTCAGCAGG AGAATCTAGC TGTCCTGCAC AGGTTAGGAA GAGCATGGCT ACGCTTTTCT
     1861 CGTGCTGCTG GTTTACATAC ATGGATTGCT CACGGGACAC TGTTGGGTTG GTATTGGAAT
     1921 GGTCTGATTC TGCCGTGGGA TCAGGATCTT GATGTTCAAA TGACTGTACA ATCATTGTAT
     1981 CTGTTGGGAA GGAATTTCAA CAGCTCTCTT GTAACTGATG TTAGTATTGA AGATGGCTAC
     2041 AGCTCAGCAT TGGGACATTA CTATATTGAC GTTGGATCCT CCTTCTTTGT TAGGGATAAA
     2101 CTAAATGGTA ACAATGCTAT AGATGCACGT TTCGTTGATA CTGAGACCGG GTTGTATGTT
     2161 GATATAACTG CATTGGCTTT TACAGATCAC TTAAAACTAA AACTCACTAC CAAAGAGAAA
     2221 GTTGAGCTAC AGAAGGTTAT GGATCCAAAT GTAAAGGAAA AATTGCAGTG GATCAAAAAT
     2281 AAATATTCAA CGGCCACGCT ACCGGGTGTG ATAGAAACAG ATAGGAATAA AGTATCTGAT
     2341 GCGCTAGAGA AGCAATTTCA TGATTTCAAG TTCGACAATT TTGTCAACAA AGAGTTGTTT
     2401 CACTGTCGAA ATAACCATTT CTACAAATAT GGAGAGGTTG GCCGATTACG GAGCACTATG
     2461 TTTGAGGGCG TTCCTGCCCT TATACCATTT GAATTTGAGT CCATACTGAA ACGAGAATAT
     2521 CCTAAAGGTC TAACTTTGAA GCATTTCTCC AATCATTTTT GGGATCCAGT GAACCGATTG
     2581 TGGGTACCAG AAAAGAAGAA AAAAATTAGA CACATAGAGT TTTCACTTAC GAAGGAAGTT
     2641 ACAGAAAGCC ACAAGAAAGA ACTTGCACAG ATCCATGGGA ACGAAACGGG TATAACCTCC
     2701 GACTTCGCAT ATTCTCCTTT CAGAATAGAT CCCTGGCTGT CTCGATACAG GAAAAAAATG
     2761 ACTAGGAGCC AATAAGTCGA CCATCATCAT CATCATCATT GAGTTTTAGC CTTAGACATG
     2821 ACTGTTCCTC AGTTCAAGTT GGGCACTTAC GAGAAGACCG GTCTTGCTAG ATTCTAATCA
     2881 AGAGGATGTC AGAATGCCAT TTGCCTGAGA GATGCAGGCT TCATTTTTGA TACTTTTTTA
     2941 TTTGTAACCT ATATAGTATA GGATTTTTTT TGTCATTTTG TTTCTTCTCG TACGAGCTTG
     3001 CTCCTGATCA GCCTATCTCG CAGCTGATGA ATATCTTGTG GTAGGGGTTT GGGAAAATCA
     3061 TTCGAGTTTG ATGTTTTTCT TGGTATTTCC CACTCCTCTT CAGAGTACAG AAGATTAAGT
     3121 GAGACCTTCG TTTGTGCGGA TCCCCCACAC ACCATAGCTT CAAAATGTTT CTACTCCTTT
     3181 TTTACTCTTC CAGATTTTCT CGGACTCCGC GCATCGCCGT ACCACTTCAA AACACCCAAG
     3241 CACAGCATAC TAAATTTTCC CTCTTTCTTC CTCTAGGGTG TCGTTAATTA CCCGTACTAA
     3301 AGGTTTGGAA AAGAAAAAAG AGACCGCCTC GTTTCTTTTT CTTCGTCGAA AAAGGCAATA
     3361 AAAATTTTTA TCACGTTTCT TTTTCTTGAA ATTTTTTTTT TTAGTTTTTT TCTCTTTCAG
     3421 TGACCTCCAT TGATATTTAA GTTAATAAAC GGTCTTCAAT TTCTCAAGTT TCAGTTTCAT
     3481 TTTTCTTGTT CTATTACAAC TTTTTTTACT TCTTGTTCAT TAGAAAGAAA GCATAGCAAT
     3541 CTAATCTAAG GGCGGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA
     3601 CGACAAGGTG AGGAACTAAA CCATGGGTAC CACTCTTGAC GACACGGCTT ACCGGTACCG
     3661 CACCAGTGTC CCGGGGGACG CCGAGGCCAT CGAGGCACTG GATGGGTCCT TCACCACCGA
     3721 CACCGTCTTC CGCGTCACCG CCACCGGGGA CGGCTTCACC CTGCGGGAGG TGCCGGTGGA
     3781 CCCGCCCCTG ACCAAGGTGT TCCCCGACGA CGAATCGGAC GACGAATCGG ACGACGGGGA
     3841 GGACGGCGAC CCGGACTCCC GGACGTTCGT CGCGTACGGG GACGACGGCG ACCTGGCGGG
     3901 CTTCGTGGTC ATCTCGTACT CGGCGTGGAA CCGCCGGCTG ACCGTCGAGG ACATCGAGGT
     3961 CGCCCCGGAG CACCGGGGGC ACGGGGTCGG GCGCGCGTTG ATGGGGCTCG CGACGGAGTT
     4021 CGCCGGCGAG CGGGGCGCCG GGCACCTCTG GCTGGAGGTC ACCAACGTCA ACGCACCGGC
     4081 GATCCACGCG TACCGGCGGA TGGGGTTCAC CCTCTGCGGC CTGGACACCG CCCTGTACGA
     4141 CGGCACCGCC TCGGACGGCG AGCGGCAGGC GCTCTACATG AGCATGCCCT GCCCCTAATC
     4201 AGTACTGACA ATAAAAAGAT TCTTGTTTTC AAGAACTTGT CATTTGTATA GTTTTTTTAT
     4261 ATTGTAGTTG TTCTATTTTA ATCAAATGTT AGCGTGATTT ATATTTTTTT TCGCCTCGAC
     4321 ATCATCTGCC CAGATGCGAA GTTAAGTGCG CAGAAAGTAA TATCATGCGT CAATCGTATG
     4381 TGAATGCTGG TCGCTATACT GCTGTCGATT CGATACTAAC GCCGCCATCC AGTGTCGAAA
     4441 ACGAGCTCGA ATTCATCGAT GATGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
     4501 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
     4561 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
     4621 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
     4681 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
     4741 GGACTGACAC GTCCGACGGC GGCCCACGGG TCCCAGGCCT CGGAGATCCG TCCCCCTTTT
     4801 CCTTTGTCGA TATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT
     4861 CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT
     4921 TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT
     4981 ACAGACGCGT GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC
     5041 GCTCGAAGGC TTTAATTTGC AAGCTGGAGA CCAACATGTG AGCAAAAGGC CAGCAAAAGG
     5101 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
     5161 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
     5221 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
     5281 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT
     5341 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
     5401 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
     5461 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
     5521 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG
     5581 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
     5641 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
     5701 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
     5761 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGCA TGAGATC
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