LOCUS       pPic9_hLAL      9175 bp    DNA     circular UNA 24-JAN-2012
DEFINITION  LMBP 11124.
ACCESSION   pPic9_hLAL
FEATURES             Location/Qualifiers
     promoter        4..951
                     /note="Oriinal location: 1..948"
                     /note="5'AOX1promotorfragment"
                     /label=5'AOX1
     primer          858..878
                     /label=5'AOX
     S_region        952..1221
                     /note="Oriinal location: 949..1218"
                     /note="secretiesignaal"
                     /label="S alpha MF prepro"
     primer          967..995
                     /label=aMF
     misc_feature    1232..2390
                     /feature_type=Insertion
                     /note="Region on donor sequence (pUC57_sshLAL): 71..1229"
                     /label=pUC57_sshLAL
     CDS             1237..2390
                     /label=hLAL
     primer          1783..1802
                     /label=hLAL-RV1
     misc_feature    2391..>9175
                     /feature_type=Insertion
                     /note="Region on donor sequence (pPIC9): 1236..1228"
                     /label=pPIC9
     terminator      2408..2741
                     /note="Oriinal location: 1253..1586"
                     /label=3'AOX1(TT)
     primer          2482..2502
                     /note="Oriinal location: 1327..1347"
                     /note="3'AOX1 primersite"
                     /label=3'AOX1
     CDS             complement(3135..5669)
                     /note="Oriinal location: complement(1980..4514)"
                     /note="HIS4 ORF"
                     /label=HIS4
     promoter        6025..6781
                     /note="Oriinal location: 4870..5626"
                     /label=3'AOX1
     rep_origin      complement(7189..7863)
                     /note="Oriinal location: complement(6034..6708)"
                     /note="ColE1 origin"
                     /label=ColE1
     CDS             complement(8008..8868)
                     /note="Oriinal location: complement(6853..7713)"
                     /note="Ampiciline resistentie"
                     /label=AMP
ORIGIN
        1 TCGAGATCTA ACATCCAAAG ACGAAAGGTT GAATGAAACC TTTTTGCCAT CCGACATCCA
       61 CAGGTCCATT CTCACACATA AGTGCCAAAC GCAACAGGAG GGGATACACT AGCAGCAGAC
      121 CGTTGCAAAC GCAGGACCTC CACTCCTCTT CTCCTCAACA CCCACTTTTG CCATCGAAAA
      181 ACCAGCCCAG TTATTGGGCT TGATTGGAGC TCGCTCATTC CAATTCCTTC TATTAGGCTA
      241 CTAACACCAT GACTTTATTA GCCTGTCTAT CCTGGCCCCC CTGGCGAGGT TCATGTTTGT
      301 TTATTTCCGA ATGCAACAAG CTCCGCATTA CACCCGAACA TCACTCCAGA TGAGGGCTTT
      361 CTGAGTGTGG GGTCAAATAG TTTCATGTTC CCCAAATGGC CCAAAACTGA CAGTTTAAAC
      421 GCTGTCTTGG AACCTAATAT GACAAAAGCG TGATCTCATC CAAGATGAAC TAAGTTTGGT
      481 TCGTTGAAAT GCTAACGGCC AGTTGGTCAA AAAGAAACTT CCAAAAGTCG CCATACCGTT
      541 TGTCTTGTTT GGTATTGATT GACGAATGCT CAAAAATAAT CTCATTAATG CTTAGCGCAG
      601 TCTCTCTATC GCTTCTGAAC CCCGGTGCAC CTGTGCCGAA ACGCAAATGG GGAAACACCC
      661 GCTTTTTGGA TGATTATGCA TTGTCTCCAC ATTGTATGCT TCCAAGATTC TGGTGGGAAT
      721 ACTGCTGATA GCCTAACGTT CATGATCAAA ATTTAACTGT TCTAACCCCT ACTTGACAGC
      781 AATATATAAA CAGAAGGAAG CTGCCCTGTC TTAAACCTTT TTTTTTATCA TCATTATTAG
      841 CTTACTTTCA TAATTGCGAC TGGTTCCAAT TGACAAGCTT TTGATTTTAA CGACTTTTAA
      901 CGACAACTTG AGAAGATCAA AAAACAACTA ATTATTCGAA GGATCCAAAC GATGAGATTT
      961 CCTTCAATTT TTACTGCAGT TTTATTCGCA GCATCCTCCG CATTAGCTGC TCCAGTCAAC
     1021 ACTACAACAG AAGATGAAAC GGCACAAATT CCGGCTGAAG CTGTCATCGG TTACTCAGAT
     1081 TTAGAAGGGG ATTTCGATGT TGCTGTTTTG CCATTTTCCA ACAGCACAAA TAACGGGTTA
     1141 TTGTTTATAA ATACTACTAT TGCCAGCATT GCTGCTAAAG AAGAAGGGGT ATCTCTCGAG
     1201 AAAAGAGAGG CTGAAGCTTA CGTAGAATTC CCTAGTGGAG GGAAACTGAC AGCTGTGGAT
     1261 CCTGAAACAA ACATGAATGT GAGTGAAATT ATCTCTTACT GGGGATTCCC TAGTGAGGAA
     1321 TACCTAGTTG AGACAGAAGA TGGATATATT CTGTGCCTTA ACCGAATTCC TCATGGGAGG
     1381 AAGAACCATT CTGACAAAGG TCCCAAACCA GTTGTCTTCC TGCAACATGG CTTGCTGGCA
     1441 GATTCTAGTA ACTGGGTCAC AAACCTTGCC AACAGCAGCC TGGGCTTCAT TCTTGCTGAT
     1501 GCTGGTTTTG ACGTGTGGAT GGGCAACAGC AGAGGAAATA CCTGGTCTCG GAAACATAAG
     1561 ACACTCTCAG TTTCTCAGGA TGAATTCTGG GCTTTCAGTT ATGATGAGAT GGCAAAATAT
     1621 GACCTACCAG CTTCCATTAA CTTCATTCTG AATAAAACTG GCCAAGAACA AGTGTATTAT
     1681 GTGGGTCATT CTCAAGGCAC CACTATAGGT TTTATAGCAT TTTCACAGAT CCCTGAGCTG
     1741 GCTAAAAGGA TTAAAATGTT TTTTGCCCTG GGTCCTGTGG CTTCCGTCGC CTTCTGTACT
     1801 AGCCCTATGG CCAAATTAGG ACGATTACCA GATCATCTCA TTAAGGACTT ATTTGGAGAC
     1861 AAAGAATTTC TTCCCCAGAG TGCGTTTTTG AAGTGGCTGG GTACCCACGT TTGCACTCAT
     1921 GTCATACTGA AGGAGCTCTG TGGAAATCTC TGTTTTCTTC TGTGTGGATT TAATGAGAGA
     1981 AATTTAAATA TGTCTAGAGT GGATGTATAT ACAACACATT CTCCTGCTGG AACTTCTGTG
     2041 CAAAACATGT TACACTGGAG CCAGGCTGTT AAATTCCAAA AGTTTCAAGC CTTTGACTGG
     2101 GGAAGCAGTG CCAAGAATTA TTTTCATTAC AACCAGAGTT ATCCTCCCAC ATACAATGTG
     2161 AAGGACATGC TTGTGCCGAC TGCAGTCTGG AGCGGGGGTC ACGACTGGCT TGCAGATGTC
     2221 TACGACGTCA ATATCTTACT GACTCAGATC ACCAACTTGG TGTTCCATGA GAGCATTCCG
     2281 GAATGGGAGC ATCTTGACTT CATTTGGGGC CTGGATGCCC CTTGGAGGCT TTATAATAAA
     2341 ATTATTAATC TAATGAGGAA ATATCAGCAT CATCATCATC ATCATTGAGC GGCCGCGAAT
     2401 TAATTCGCCT TAGACATGAC TGTTCCTCAG TTCAAGTTGG GCACTTACGA GAAGACCGGT
     2461 CTTGCTAGAT TCTAATCAAG AGGATGTCAG AATGCCATTT GCCTGAGAGA TGCAGGCTTC
     2521 ATTTTTGATA CTTTTTTATT TGTAACCTAT ATAGTATAGG ATTTTTTTTG TCATTTTGTT
     2581 TCTTCTCGTA CGAGCTTGCT CCTGATCAGC CTATCTCGCA GCTGATGAAT ATCTTGTGGT
     2641 AGGGGTTTGG GAAAATCATT CGAGTTTGAT GTTTTTCTTG GTATTTCCCA CTCCTCTTCA
     2701 GAGTACAGAA GATTAAGTGA GAAGTTCGTT TGTGCAAGCT TATCGATAAG CTTTAATGCG
     2761 GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGGCACC GTGTATGAAA TCTAACAATG
     2821 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC
     2881 CGGTACTGCC GGGCCTCTTG CGGGATATCG TCCATTCCGA CAGCATCGCC AGTCACTATG
     2941 GCGTGCTGCT AGCGCTATAT GCGTTGATGC AATTTCTATG CGCACCCGTT CTCGGAGCAC
     3001 TGTCCGACCG CTTTGGCCGC CGCCCAGTCC TGCTCGCTTC GCTACTTGGA GCCACTATCG
     3061 ACTACGCGAT CATGGCGACC ACACCCGTCC TGTGGATCTA TCGAATCTAA ATGTAAGTTA
     3121 AAATCTCTAA ATAATTAAAT AAGTCCCAGT TTCTCCATAC GAACCTTAAC AGCATTGCGG
     3181 TGAGCATCTA GACCTTCAAC AGCAGCCAGA TCCATCACTG CTTGGCCAAT ATGTTTCAGT
     3241 CCCTCAGGAG TTACGTCTTG TGAAGTGATG AACTTCTGGA AGGTTGCAGT GTTAACTCCG
     3301 CTGTATTGAC GGGCATATCC GTACGTTGGC AAAGTGTGGT TGGTACCGGA GGAGTAATCT
     3361 CCACAACTCT CTGGAGAGTA GGCACCAACA AACACAGATC CAGCGTGTTG TACTTGATCA
     3421 ACATAAGAAG AAGCATTCTC GATTTGCAGG ATCAAGTGTT CAGGAGCGTA CTGATTGGAC
     3481 ATTTCCAAAG CCTGCTCGTA GGTTGCAACC GATAGGGTTG TAGAGTGTGC AATACACTTG
     3541 CGTACAATTT CAACCCTTGG CAACTGCACA GCTTGGTTGT GAACAGCATC TTCAATTCTG
     3601 GCAAGCTCCT TGTCTGTCAT ATCGACAGCC AACAGAATCA CCTGGGAATC AATACCATGT
     3661 TCAGCTTGAG ACAGAAGGTC TGAGGCAACG AAATCTGGAT CAGCGTATTT ATCAGCAATA
     3721 ACTAGAACTT CAGAAGGCCC AGCAGGCATG TCAATACTAC ACAGGGCTGA TGTGTCATTT
     3781 TGAACCATCA TCTTGGCAGC AGTAACGAAC TGGTTTCCTG GACCAAATAT TTTGTCACAC
     3841 TTAGGAACAG TTTCTGTTCC GTAAGCCATA GCAGCTACTG CCTGGGCGCC TCCTGCTAGC
     3901 ACGATACACT TAGCACCAAC CTTGTGGGCA ACGTAGATGA CTTCTGGGGT AAGGGTACCA
     3961 TCCTTCTTAG GTGGAGATGC AAAAACAATT TCTTTGCAAC CAGCAACTTT GGCAGGAACA
     4021 CCCAGCATCA GGGAAGTGGA AGGCAGAATT GCGGTTCCAC CAGGAATATA GAGGCCAACT
     4081 TTCTCAATAG GTCTTGCAAA ACGAGAGCAG ACTACACCAG GGCAAGTCTC AACTTGCAAC
     4141 GTCTCCGTTA GTTGAGCTTC ATGGAATTTC CTGACGTTAT CTATAGAGAG ATCAATGGCT
     4201 CTCTTAACGT TATCTGGCAA TTGCATAAGT TCCTCTGGGA AAGGAGCTTC TAACACAGGT
     4261 GTCTTCAAAG CGACTCCATC AAACTTGGCA GTTAGTTCTA AAAGGGCTTT GTCACCATTT
     4321 TGACGAACAT TGTCGACAAT TGGTTTGACT AATTCCATAA TCTGTTCCGT TTTCTGGATA
     4381 GGACGACGAA GGGCATCTTC AATTTCTTGT GAGGAGGCCT TAGAAACGTC AATTTTGCAC
     4441 AATTCAATAC GACCTTCAGA AGGGACTTCT TTAGGTTTGG ATTCTTCTTT AGGTTGTTCC
     4501 TTGGTGTATC CTGGCTTGGC ATCTCCTTTC CTTCTAGTGA CCTTTAGGGA CTTCATATCC
     4561 AGGTTTCTCT CCACCTCGTC CAACGTCACA CCGTACTTGG CACATCTAAC TAATGCAAAA
     4621 TAAAATAAGT CAGCACATTC CCAGGCTATA TCTTCCTTGG ATTTAGCTTC TGCAAGTTCA
     4681 TCAGCTTCCT CCCTAATTTT AGCGTTCAAC AAAACTTCGT CGTCAAATAA CCGTTTGGTA
     4741 TAAGAACCTT CTGGAGCATT GCTCTTACGA TCCCACAAGG TGGCTTCCAT GGCTCTAAGA
     4801 CCCTTTGATT GGCCAAAACA GGAAGTGCGT TCCAAGTGAC AGAAACCAAC ACCTGTTTGT
     4861 TCAACCACAA ATTTCAAGCA GTCTCCATCA CAATCCAATT CGATACCCAG CAACTTTTGA
     4921 GTTGCTCCAG ATGTAGCACC TTTATACCAC AAACCGTGAC GACGAGATTG GTAGACTCCA
     4981 GTTTGTGTCC TTATAGCCTC CGGAATAGAC TTTTTGGACG AGTACACCAG GCCCAACGAG
     5041 TAATTAGAAG AGTCAGCCAC CAAAGTAGTG AATAGACCAT CGGGGCGGTC AGTAGTCAAA
     5101 GACGCCAACA AAATTTCACT GACAGGGAAC TTTTTGACAT CTTCAGAAAG TTCGTATTCA
     5161 GTAGTCAATT GCCGAGCATC AATAATGGGG ATTATACCAG AAGCAACAGT GGAAGTCACA
     5221 TCTACCAACT TTGCGGTCTC AGAAAAAGCA TAAACAGTTC TACTACCGCC ATTAGTGAAA
     5281 CTTTTCAAAT CGCCCAGTGG AGAAGAAAAA GGCACAGCGA TACTAGCATT AGCGGGCAAG
     5341 GATGCAACTT TATCAACCAG GGTCCTATAG ATAACCCTAG CGCCTGGGAT CATCCTTTGG
     5401 ACAACTCTTT CTGCCAAATC TAGGTCCAAA ATCACTTCAT TGATACCATT ATTGTACAAC
     5461 TTGAGCAAGT TGTCGATCAG CTCCTCAAAT TGGTCCTCTG TAACGGATGA CTCAACTTGC
     5521 ACATTAACTT GAAGCTCAGT CGATTGAGTG AACTTGATCA GGTTGTGCAG CTGGTCAGCA
     5581 GCATAGGGAA ACACGGCTTT TCCTACCAAA CTCAAGGAAT TATCAAACTC TGCAACACTT
     5641 GCGTATGCAG GTAGCAAGGG AAATGTCATA CTTGAAGTCG GACAGTGAGT GTAGTCTTGA
     5701 GAAATTCTGA AGCCGTATTT TTATTATCAG TGAGTCAGTC ATCAGGAGAT CCTCTACGCC
     5761 GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC
     5821 GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC
     5881 GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA
     5941 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
     6001 CAGGAGTCGC ATAAGGGAGA GCGTCGAGTA TCTATGATTG GAAGTATGGG AATGGTGATA
     6061 CCCGCATTCT TCAGTGTCTT GAGGTCTCCT ATCAGATTAT GCCCAACTAA AGCAACCGGA
     6121 GGAGGAGATT TCATGGTAAA TTTCTCTGAC TTTTGGTCAT CAGTAGACTC GAACTGTGAG
     6181 ACTATCTCGG TTATGACAGC AGAAATGTCC TTCTTGGAGA CAGTAAATGA AGTCCCACCA
     6241 ATAAAGAAAT CCTTGTTATC AGGAACAAAC TTCTTGTTTC GAACTTTTTC GGTGCCTTGA
     6301 ACTATAAAAT GTAGAGTGGA TATGTCGGGT AGGAATGGAG CGGGCAAATG CTTACCTTCT
     6361 GGACCTTCAA GAGGTATGTA GGGTTTGTAG ATACTGATGC CAACTTCAGT GACAACGTTG
     6421 CTATTTCGTT CAAACCATTC CGAATCCAGA GAAATCAAAG TTGTTTGTCT ACTATTGATC
     6481 CAAGCCAGTG CGGTCTTGAA ACTGACAATA GTGTGCTCGT GTTTTGAGGT CATCTTTGTA
     6541 TGAATAAATC TAGTCTTTGA TCTAAATAAT CTTGACGAGC CAAGGCGATA AATACCCAAA
     6601 TCTAAAACTC TTTTAAAACG TTAAAAGGAC AAGTATGTCT GCCTGTATTA AACCCCAAAT
     6661 CAGCTCGTAG TCTGATCCTC ATCAACTTGA GGGGCACTAT CTTGTTTTAG AGAAATTTGC
     6721 GGAGATGCGA TATCGAGAAA AAGGTACGCT GATTTTAAAC GTGAAATTTA TCTCAAGATC
     6781 TCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
     6841 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG
     6901 CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT
     6961 ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
     7021 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC
     7081 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
     7141 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA
     7201 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
     7261 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     7321 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     7381 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     7441 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     7501 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     7561 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     7621 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     7681 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     7741 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     7801 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     7861 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     7921 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     7981 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     8041 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     8101 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
     8161 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     8221 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     8281 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC
     8341 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     8401 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     8461 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     8521 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     8581 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC
     8641 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     8701 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     8761 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     8821 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     8881 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     8941 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA
     9001 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC
     9061 CTTTCGTCTT CAAGAATTAA TTCTCATGTT TGACAGCTTA TCATCGATAA GCTGACTCAT
     9121 GTTGGTATTG TGAAATAGAC GCAGATCGGG AACACTGAAA AATAACAGTT ATTAT
//

