LOCUS       pD603-HA-LZTR1-R466Q        7532 bp    DNA     circular SYN 24-JAN-2019
DEFINITION  LMBP 11169.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) 
            format
            http://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12655425|
COMMENT     VNTDBDATE|-12655425|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pD603-HA-LZTR1-R466Q|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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FEATURES             Location/Qualifiers
     rep_origin      281..356
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             448..1242
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             complement(1590..2450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4715..4731
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     4773..4799
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /vntifkey="22"
                     /label=HA\epitope
     terminator      7350..7461
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        4087..4670
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3084..3486
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      1416..1490
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1416..1490)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        43..324
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             4800..7322
                     /vntifkey="4"
                     /label=hLZTR1\mutant
BASE COUNT     1777 a      1997 c      1965 g      1793 t 
ORIGIN
        1 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 
       61 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
      121 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
      301 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 
      361 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 
      421 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 
      481 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 
      541 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 
      601 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc 
      661 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 
      721 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 
      781 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 
      841 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 
      901 cttgtcgatc aggatgatct ggacgaggaa catcaggggc tcgcgccagc cgaactgttc 
      961 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 
     1021 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 
     1081 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaggaa 
     1141 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 
     1201 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg 
     1261 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct 
     1321 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 
     1381 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg 
     1441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     1501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct 
     1561 ctagctagag cttggcgtaa tcatggtcat taccaatgct taatcagtga ggcacctatc 
     1621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     1681 acgatacggg agggcttacc atctggcccc agcgctgcga tgataccgcg agaaccacgc 
     1741 tcaccggctc cggatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     1801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     1861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca tcgctacagg catcgtggtg 
     1921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     1981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     2041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     2101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     2161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 
     2221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     2281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     2341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     2401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat attcttcctt 
     2461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     2521 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac 
     2581 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa 
     2641 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga 
     2701 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     2761 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     2821 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     2881 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     2941 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     3001 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     3061 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     3121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     3181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     3241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     3301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     3361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     3421 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     3481 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc 
     3541 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg 
     3601 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct 
     3661 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg 
     3721 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa 
     3781 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc 
     3841 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga 
     3901 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc 
     3961 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag 
     4021 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt 
     4081 ggcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 
     4141 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 
     4201 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 
     4261 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 
     4321 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 
     4381 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 
     4441 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 
     4501 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 
     4561 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 
     4621 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa 
     4681 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat aggggagaga 
     4741 ccacacccaa gctgtctaga gccgccacca tgtacccata cgatgttcca gattacgcta 
     4801 tggctggacc gggcagcacg ggaggacaga tcggggctgc ggccctggca ggcggcgcgc 
     4861 ggtccaaggt agccccgagc gtggacttcg accatagctg ctcggacagt gtcgagtacc 
     4921 tgacgctcaa cttcgggccc ttcgaaacag tgcatcgctg gcggcgcctc ccgccctgcg 
     4981 acgagttcgt gggtgcccgg cgcagcaagc acacagtggt ggcctataaa gatgccattt 
     5041 atgtatttgg tggagacaat gggaagacca tgctcaatga cctcctgcgg ttcgatgtga 
     5101 aagactgctc ctggtgcagg gcctttacca ctgggacccc accggccccc cgttaccacc 
     5161 actcggccgt cgtctatggg agcagcatgt ttgtctttgg gggttacact ggggacattt 
     5221 attccaattc taacttgaag aataaaaacg acctctttga atacaagttt gcaactggcc 
     5281 agtggacgga gtggaaaatt gaaggacggt tgccagtcgc taggtcagcc catggggcca 
     5341 cggtgtacag tgacaagctg tggatctttg ctggctatga cggcaacgcc aggttgaatg 
     5401 acatgtggac aattggcctc caggaccgag agctcacatg ctgggaggag gtggcccaga 
     5461 gtggcgagat ccccccatct tgctgcaact tccccgtggc tgtgtgccgg gacaagatgt 
     5521 ttgtattctc tgggcaaagc ggagccaaaa taaccaacaa cctcttccag tttgaattca 
     5581 aggacaagac gtggacacgc atcccaactg aacatctgct ccggggctcc ccaccacccc 
     5641 cgcagcggcg ctacgggcat accatggtgg cctttgaccg ccacctctat gtgtttgggg 
     5701 gtgcggccga caacacgctg cccaacgagc tgcactgcta tgacgtggac ttccagacct 
     5761 gggaggtcgt ccagcccagc tccgacagcg aggttggtgg ggctgaagtg cccgagcgag 
     5821 cctgtgcttc cgaggaggtg cccaccctga cctatgagga gcgggttggc ttcaagaagt 
     5881 cccgagatgt gtttggcctg gactttggca ccacctcagc caagcagccc acccagcctg 
     5941 cctcggagct gcccagtggg agattattcc acgcggctgc tgtcatctcg gacgccatgt 
     6001 acatcttcgg gggcacggtg gacaacaaca tccgcagcgg ggagatgtac aggttccagt 
     6061 tctcctgtta ccctaaatgc acgctgcacg aggactacgg gcggctgtgg gagagccgcc 
     6121 agttctgcga cgtggagttc gtgctgggtg agaaggagga gtgcgtgcag ggccacgtag 
     6181 ccattgtcac agcgcaaagc cgctggcttc gaagaaagat cactcaggca cgagagagac 
     6241 tcgcacagaa acttgagcaa gaagccgcac cagttccaag agaagctcca ggcgtggctg 
     6301 ctggtggagc ccgaccaccc ctgctgcacg tggccatccg ggaagccgag gcccgaccct 
     6361 tcgaggtgct catgcagttc ctctacaccg acaagatcaa atacccacgg aaaggccatg 
     6421 tggaggatgt gctgctcatc atggatgtgt acaaactggc actgagcttc cagttgtgcc 
     6481 gcctggagca gctgtgccgc cagtacatcg aggcctccgt ggacctgcag aacgtgctgg 
     6541 ttgtgtgcga gagtgccgcc cggctgcagc tgagccaact caaggagcac tgcctgaact 
     6601 tcgtggtaaa ggagtcccac ttcaaccagg taatcatgat gaaggagttc gagcgcctct 
     6661 cctctccact gatagtggag attgtgcggc ggaagcagca gccgccccct cgcactccct 
     6721 tggaccagcc agtggacatt ggcacatctc tgatccagga catgaaggca tacctggagg 
     6781 gagcgggcgc ggaattctgt gacatcactc tgttgcttga cgggcaccca cggccagccc 
     6841 acaaggctat cctggccgcc cgctccagct actttgaagc catgttccgg tccttcatgc 
     6901 ccgaagatgg gcaggtaaac atctccatcg gggagatggt gcccagcagg caggccttcg 
     6961 agtccatgct gcgctacatc tactacggcg aggtcaacat gccgcccgag gactcgctct 
     7021 acttgtttgc ggccccctac tactacggct tctacaacaa ccggctgcag gcgtactgca 
     7081 agcagaacct ggagatgaac gtgacggtgc agaacgtgct gcagatcctg gaggcagctg 
     7141 acaaaacgca ggcactggac atgaagcggc actgcctgca catcattgtg caccagttca 
     7201 ccaaggtatc caagttgccc accctgcggt cgctgagcca gcagctgctg ctggacatca 
     7261 tagactccct ggcctcccac atctcagaca agcagtgcgc agagctgggc gccgacatct 
     7321 aaggttgagg tctctaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     7381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     7441 ataaaatgag gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta 
     7501 taagggattt tgccgatttc ggcctattgg tt 
//
