LOCUS       pLenti-CMV-HA-LZTR1-S122L-puro       10418 bp ds-DNA     circular SYN 24-JAN-2019
DEFINITION  LMBP 11171.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|867456000|
COMMENT     VNTDBDATE|867456000|
COMMENT     VNTNAME|pLenti-CMV-HA-LZTR1-S122L-puro|
FEATURES             Location/Qualifiers
     CDS             134..733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
     LTR             871..1104
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1176..1297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1337..1472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(1493..1511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1521..1537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1679..2134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2160..2264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2265..3125
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      3296..3884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4172..4193
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4208..4238
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4246..4262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4270..4286
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4307..4325
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4353..4579
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4580..4760
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4807..4932
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5425..5658
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6154..6270
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6293..6596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6597..6800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6886..6912
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
     CDS             7411..7422
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
     regulatory      7775..7784
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    9442..10030
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(9913..9924)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
     gap             10417..10430
                     /estimated_length=14
ORIGIN
        1 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
       61 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
      121 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
      301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
      361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
      421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
      481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
      541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
      601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
      661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
      721 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
      781 ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
      841 ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc caacgaagac
      901 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
      961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1081 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     1141 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     1201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     1261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     1321 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     1381 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     1441 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     1501 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     1561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     1621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     1681 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1741 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1801 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1861 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1921 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1981 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2041 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2101 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4141 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4201 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4261 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     4321 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     4381 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     4441 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     4501 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     4561 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     4621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4741 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     4801 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     4861 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     4921 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     4981 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     5041 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     5101 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     5161 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     5221 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     5281 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     5341 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5401 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5461 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     5521 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     5581 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     5641 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     5701 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     5761 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     5821 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     5881 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     5941 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6001 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     6061 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     6121 attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg
     6181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     6241 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
     6301 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6361 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6421 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6481 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6541 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6601 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6661 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6721 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6781 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     6841 gctgttttga cctccataga agacaccgac tctagaggat ccatgtaccc atacgatgtt
     6901 ccagattacg ctatggctgg accgggcagc acaggtgggc agatcggggc tgcggccctg
     6961 gcaggcggcg cgcggtccaa ggtagccccg agcgtggact tcgaccatag ctgctcggac
     7021 agtgtcgagt acctgacgct caacttcggg cccttcgaaa cagtgcatcg ctggcggcgc
     7081 ctcccgccct gcgacgagtt cgtgggtgcc cggcgcagca agcacacagt ggtggcctat
     7141 aaagatgcca tttatgtatt tggtggagac aatgggaaga ccatgctcaa tgacctcctg
     7201 cggttcgatg tgaaagactg ctcctggtgc agggccttta ccactgggac cccaccggcc
     7261 ccccgttacc accacctcgc cgtcgtctat gggagcagca tgtttgtctt tgggggttac
     7321 actggggaca tttattccaa ttctaacttg aagaataaaa acgacctctt tgaatacaag
     7381 tttgcaactg gccagtggac ggagtggaaa attgaaggac ggttgccagt cgctaggtca
     7441 gcccatgggg ccacggtgta cagtgacaag ctgtggatct ttgctggcta tgacggcaac
     7501 gccaggttga atgacatgtg gacaattggc ctccaggacc gagagctcac atgctgggag
     7561 gaggtggccc agagtggcga gatcccccca tcttgctgca acttccccgt ggctgtgtgc
     7621 cgggacaaga tgtttgtatt ctctgggcaa agcggagcca aaataaccaa caacctcttc
     7681 cagtttgaat tcaaggacaa gacgtggaca cgcatcccaa ctgaacacct actccggggc
     7741 tccccaccac ccccgcagcg gcgctacggg cataccatgg tggcctttga ccgccacctc
     7801 tatgtgtttg ggggtgcggc cgacaacacg ctgcccaacg agctgcactg ctatgacgtg
     7861 gacttccaga cctgggaggt cgtccagccc agctccgaca gcgaggttgg tggggctgaa
     7921 gtgcccgagc gagcctgtgc ttccgaggag gtgcccaccc tgacctatga ggagcgggtt
     7981 ggcttcaaga agtcccgaga tgtgtttggc ctggactttg gcaccacctc agccaagcag
     8041 cccacccagc ctgcctcgga gctgcccagt gggagattat tccacgcggc tgctgtcatc
     8101 tcggacgcca tgtacatctt cgggggcacg gtggacaaca acatccgcag cggggagatg
     8161 tacaggttcc agttctcctg ttaccctaaa tgcacgctgc acgaggacta cgggcggctg
     8221 tgggagagcc gccagttctg cgacgtggag ttcgtgctgg gtgagaagga ggagtgcgtg
     8281 cagggccacg tagccattgt cacagcgcgg agccgctggc ttcgaagaaa gatcactcag
     8341 gcacgagaga gactcgcaca gaaacttgag caagaagccg caccagttcc aagagaagct
     8401 ccaggcgtgg ctgctggtgg agcccgacca cccctgctgc acgtggccat ccgggaagcc
     8461 gaggcccgac ccttcgaggt gctcatgcag ttcctctaca ccgacaagat caaataccca
     8521 cggaaaggcc atgtggagga tgtgctgctc atcatggatg tgtacaaact ggcactgagc
     8581 ttccagttgt gccgcctgga gcagctgtgc cgccagtaca tcgaggcctc cgtggacctg
     8641 cagaacgtgc tggttgtgtg cgagagtgcc gcccggctgc agctgagcca actcaaggag
     8701 cactgcctga acttcgtggt aaaggagtcc cacttcaacc aggtgatcat gatgaaggag
     8761 ttcgagcgcc tctcctctcc actgatagtg gagattgtgc ggcggaagca gcagccgccc
     8821 cctcgcactc ccttggacca gccagtggac attggcacat ctctgatcca ggacatgaag
     8881 gcatacctgg agggagcggg cgcggaattc tgtgacatca ctctgttgct tgacgggcac
     8941 ccacggccag cccacaaggc tatcctggcc gcccgctcca gctactttga agccatgttc
     9001 cggtccttta tgcctgagga tgggcaggtt aacatctcca tcggggagat ggtgcccagc
     9061 aggcaggcct tcgagtccat gctgcgctac atctactacg gcgaggtcaa catgccgccc
     9121 gaggactcgc tctacttgtt tgcggccccc tactactacg gcttctacaa caaccggctg
     9181 caggcgtact gcaagcagaa cctggagatg aacgtgacgg tgcagaacgt gctgcagatc
     9241 ctggaggcag ctgacaaaac gcaggcactg gacatgaagc ggcactgcct gcacatcatt
     9301 gtgcaccagt tcaccaaggt ttccaagttg cccaccctgc ggtcgctgag ccagcagctg
     9361 ctgctggaca tcatagactc cctggcctcc cacatctcag acaagcagtg cgcagagctg
     9421 ggcgccgaca tctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     9481 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     9541 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     9601 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     9661 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     9721 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     9781 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     9841 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     9901 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     9961 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    10021 gcctccccgc ctggaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    10081 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    10141 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    10201 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    10261 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    10321 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    10381 ctgggctcag aggctgggaa ggggtgggtc cgggggcg
//
