LOCUS       pYES2-10His-rAQP        6633 bp    DNA     circular     14-MAR-2019
DEFINITION  LMBP 11182.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12726355|
COMMENT     VNTDBDATE|-12726366|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pYES2-10His-rAQP8|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 3.17558 3.26285) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
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COMMENT                                   "@N" 1 4.43791 -13.9386 0 -10 4.88388 1.16519 
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FEATURES             Location/Qualifiers
     promoter        3184..3200
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             complement(2125..2928)
                     /vntifkey="4"
                     /label=URA3
     CDS             complement(1169..2029)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3745..4690)
                     /vntifkey="33"
                     /label=2mu\ori
     rep_origin      complement(4691..5146)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        5168..5559
                     /vntifkey="29"
                     /label=GAL1\promoter
     promoter        5641..5657
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     5682..5711
                     /vntifkey="22"
                     /label=His-tag
     rep_origin      complement(226..628)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      6523..165
                     /vntifkey="43"
                     /label=CYC1\terminator
     CDS             5745..6522
                     /vntifkey="4"
                     /label=rAQP8
BASE COUNT     1791 a      1521 c      1459 g      1862 t 
ORIGIN
        1 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt 
       61 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc 
      121 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctgcg gccctgcatt 
      181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
      241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
      301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
      361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
      421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
      481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
      541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
      601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
      661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
      721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
      781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
      841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
      901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
      961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     1021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     1081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     1141 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     1201 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     1261 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     1321 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     1381 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     1441 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     1501 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     1561 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     1621 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     1681 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     1741 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     1801 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     1861 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     1921 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     1981 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     2041 ttcaatgggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca 
     2101 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc 
     2161 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag 
     2221 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata 
     2281 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca 
     2341 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc 
     2401 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc 
     2461 cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc 
     2521 cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc 
     2581 tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat 
     2641 gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag 
     2701 cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa 
     2761 acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc 
     2821 caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac 
     2881 aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg 
     2941 tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc 
     3001 ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc 
     3061 cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga 
     3121 ataaaaaaaa aatgatgaat tgaaaagcta gcttatcgat gataagctgt caaacatgag 
     3181 aattaattcc acggactata gactatacct agtatactcc gtctactgta cgatacactt 
     3241 ccgctcaggt ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca 
     3301 gctcagcaaa ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga 
     3361 ccgagaaaga gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc 
     3421 gatgtgacgc tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat 
     3481 atccgacaaa ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa 
     3541 catccgaacc tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat 
     3601 atagtctagc gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca 
     3661 tctattgcat aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt 
     3721 gcacttcaat agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa 
     3781 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag 
     3841 aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac 
     3901 aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag 
     3961 aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt 
     4021 tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt 
     4081 ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt 
     4141 aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc 
     4201 acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca 
     4261 tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag 
     4321 cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata 
     4381 tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt 
     4441 cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg 
     4501 tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata 
     4561 gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca 
     4621 atgggaagct ccaccccggt tgataatcag aaaagcccca aaaacaggaa gattgtataa 
     4681 gcaaatattt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
     4741 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
     4801 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
     4861 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
     4921 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
     4981 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
     5041 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
     5101 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg atcgatccac 
     5161 tagtccacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc 
     5221 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg 
     5281 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc 
     5341 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata 
     5401 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg 
     5461 atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac 
     5521 attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta 
     5581 atatacctct atactttaac gtcaaggaga aaaaaccccg gatcggacta ctagcagctg 
     5641 taatacgact cactataggg aatattaagc ttaccatggg ccatcatcat catcatcatc 
     5701 atcatcatca cagcagcggc catatcgaag gtcgtcacga attcatgtct ggggagacgc 
     5761 cgatgtgtag tatggaccta cgtgagatca aggggaagga gaccaacatg gctgacagtt 
     5821 accatggcat gtcctggtat gagcagtaca tacaaccgtg tgtggtggaa cttttgggct 
     5881 ccgctctctt catcttcatt gggtgtctat cggtcatcga gaacagtcca aatactgggc 
     5941 tcctgcagcc tgccctggct catgggctgg ccttggggct catcattgct accttgggga 
     6001 acatcagcgg tggacacttc aaccctgctg tgtcgctggc agtcacactg gttggaggcc 
     6061 tcaagaccat gctgctaatt ccctactggg tctcccagct gtttggggga atgatcggag 
     6121 ctgccctggc taaggtggtg agtccagagg aaaggttctg gaatgcgtct ggggcagcct 
     6181 ttgccatagt ccaggagcag gagcaggtgg cagaagccct gggggtagag atcgttatga 
     6241 cgatgctgtt ggtattggct gtgtgtatgg gtgccgtcaa tgagaagaca atgggtcccc 
     6301 tagccccatt ctccattggt ttctctgtca ttgtggatat cctggcaggt ggtgggatct 
     6361 ctggagcctg catgaaccct gctcgtgcct ttggacctgc tgtgatggct ggctactggg 
     6421 acttccattg gatctactgg ctgggcccac tcctggctgg cctctttgtg ggactgctca 
     6481 ttaggctctt cattggagat gagaaaaccc gcctgattct aaagtcgagg tgatctagag 
     6541 ggccgcatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct 
     6601 ctaaccgaaa aggaaggagt tagacaacct gaa 
//
