LOCUS       pFA-HAH1-MAL2P          6667 bp    DNA     circular     15-MAY-2020
DEFINITION  LMBP 11186.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11186
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-MAY-2020
            NGS data implemented
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13342325|
COMMENT     VNTDBDATE|-13342356|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pFA-HAH1-MAL2P|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
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FEATURES             Location/Qualifiers
     CDS             complement(5340..6200)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        6651..6667
                     /vntifkey="30"
                     /label=SP6\promoter
     promoter        complement(4307..4323)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      complement(4397..4799)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1377..2783
                     /vntifkey="4"
                     /label=ARG4
     promoter        3733..4268
                     /vntifkey="29"
                     /label=MAL2\promoter
     misc_feature    47..915
                     /vntifkey="21"
                     /label=HIS1
     misc_feature    2875..3728
                     /vntifkey="21"
                     /label=HIS1
BASE COUNT     2009 a      1275 c      1395 g      1988 t 
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcctg gaggatgagg 
       61 agacagaagt tagtagtaac aatttgggga cagaagagga tacagtgaca tcagaggaag 
      121 aagacagcga aggtatagtc aaaaagcaat aggacagcaa gacaatttat ttatttaata 
      181 gttttttata aatgtaaaat ctatcacaac atgtgtactt aattttctgt ggtaggggag 
      241 gccatttcgt tgaaacgtgt cgcgagcggt tgtgagaatt gactcatttt gattattacg 
      301 caaccattga aattttttat ttttttttgg tgaagatttt tcccacacaa cttcttcttt 
      361 tacttaacca ccaactaccg ataatggatt tagtcaatca tttaccagac cgtttgttat 
      421 ttgctgttcc taaaaagggc agattatacg aaaaatgctg taacttattg agtggtgccg 
      481 atatacagtt tagaagatct aatagattag atatagcact ttctacaaac ttgccaattg 
      541 cattaatctt cttgcctgca gctgatatcc cagttttcgt tggagaaggt aattgtgact 
      601 tgggtataac tgggttagac caaatcaaag aagctgaaca attcgacaac atcgaggact 
      661 tgttggattt gaaatttggt tcatgcaaat tgcagatcca agttccagca gatggcgagt 
      721 acgaaaagcc agaacagctt gttggaaaga aaattgtgtc ttcatttaca aaattgagta 
      781 ccgactattt caaacaattg tcagacaaac ctactaatat cagatatgtc ggtggttccg 
      841 ttgaggcttc ttgtgccttg ggtgttgctg atgctattgt cgatttggtt gaaagtggtg 
      901 aaactatgaa agcaggatcc cccctttagt aagatttttc aagagtagtc tcaaataaac 
      961 cagaatgaag gattgaaggt tttattcaat aaataattgg acagaaagtt attgtacagt 
     1021 tttaacaaca acaatttcaa aatttcaatt tatgacatat tgaccgacat aattcagtca 
     1081 tttctaatat tcaaataaca agacaccttg tataaatgaa tccacaatcg tatatgaaca 
     1141 accaaatttt atgactaact ttgggtcgtg tacttttgac gattgagtca tttttaccaa 
     1201 ttaggtaatt gaattttgtt aatttgtcat taaccgcgct ggccatccca acaataacaa 
     1261 caatcaattt aaaaacttga caaggcaaaa aaaaaaaaat tttatctttg aaaaattttt 
     1321 cttccttttc ttcttctgtt ctttacataa cagctatcaa gaataatcaa ttaataatgt 
     1381 cacaacaaca agataaacaa cctagtgaaa ataaattatg gggtggtcgt ttcaccggtg 
     1441 ccactgatcc attgatggat ttatataatg cttcattacc ttatgataaa gttatgtatg 
     1501 atgctgattt aactggtact aaagtataca cacaaggatt aaacaaatta ggattaatta 
     1561 ccactgaaga attgcatttg attcatcaag gattagaaca aatcagacaa gaatggcatg 
     1621 ataataaatt cattattaag gcaggtgatg aggatattca tactgctaat gaacgtcgat 
     1681 taggagaaat cattgggaaa aacatttctg gtaaagttca cactggtaga tcaagaaatg 
     1741 atcaagttgc taccgatatg agaattttcg ttcgtgaatc attacttaat ttaagtaaaa 
     1801 ttttacatca attcatcacg gcaattcttg aacgggcaca taaagaaatc gatgtattaa 
     1861 tgcctggtta tactcattta caaaaagctc aacctattag atgggcacat tggttaagtt 
     1921 catatgcaac ttatttcacc gaagattata aacgtttaca agaaattata actcgtgtta 
     1981 accaatctcc attaggatct ggtgctttag caggtcatcc ttatggtatt gatcgtgaat 
     2041 ttttagctaa aggattagga tttgatggtg tcattgggaa ttctttaact gcagtttcag 
     2101 atcgtgattt tgttgttgaa agtttatttt ggagtacatt atttatgaat catatttctc 
     2161 gattctcaga agatctaatt atttattcta gtggtgaatt tgggttcata aaattagctg 
     2221 atgcttattc tactggttct tcattgatgc cacaaaagaa aaaccctgac tcattagaat 
     2281 tattacgtgg gaaaagtggt agagtatttg gtcaattatc tggattttta atgtcgatca 
     2341 aatctattcc atctacttat aataaagata tgcaagagga taaagaacca ttatttgatg 
     2401 cattgactac agtggaacat tcaattttaa ttgctactgg tgtgatttct actttactga 
     2461 ttgataaaca aaatatggaa aaagctttaa ctatggatat gttggctact gatttagctg 
     2521 attatttagt tagaaaagga gttccattta gagaaactca tcatatatct ggtgaatgtg 
     2581 ttagaaaagc tgaagaagaa aaattatctg gaattgatca attatctttt gaacaatttc 
     2641 aacaaattga ttctcgtttt gaaaaagacg ttatggaaac ttttgatttt gaagctagtg 
     2701 tggaaagaag agatgctatt ggtggtactg ctaaaagtgc cgttttaaaa caattagaaa 
     2761 atttaaaatc aattttaagt taatttataa atagtcatat aataatcaca gtattgttta 
     2821 acttacataa aatataatca attaacacag agataccttg tactacaata gtttctgata 
     2881 tcccagtttt cgttggagaa ggtaattgtg acttgggtat aactgggtta gaccaaatca 
     2941 aagaagctga acaattcgac aacatcgagg acttgttgga tttgaaattt ggttcatgca 
     3001 aattgcagat ccaagttcca gcagatggcg agtacgaaaa gccagaacag cttgttggaa 
     3061 agaaaattgt gtcttcattt acaaaattga gtaccgacta tttcaaacaa ttgtcagaca 
     3121 aacctactaa tatcagatat gtcggtggtt ccgttgaggc ttcttgtgcc ttgggtgttg 
     3181 ctgatgctat tgtcgatttg gttgaaagtg gtgaaactat gaaagcagct gggttaaagg 
     3241 ccatagaaac catattggag acttcggctc atttgatttc gtcaaaaaaa agtaaattcc 
     3301 cagaaatggt caatataatc gtccaaagac ttcaaggtgt tttagctgcc caggaatatg 
     3361 tcttgtgtaa ctacaatgcc ccaaaatcca tccaagcaaa atgtttaacc attactccag 
     3421 gcagaagggc cgctactgtc tctactttgg acaaacacag cgacgacgaa gaggactggg 
     3481 ttgccatctc atccatggtt aatagaaagg aaatcggtaa tgtaatggac gaattgaaga 
     3541 aagctggtgc aaccgatata ttggtactcg agatatcaaa ttgtagagtt taaaagaagt 
     3601 gatagtttct cataaatatt atacatatat acttatagat ctacttcaat ttgactcaat 
     3661 ctgtcttata ccatctttat ctaccacctt gatgtacaca cctttaaagt caataggcat 
     3721 tctcgtttaa acttttgtct agtaccatct gtaccattac acttaaatta tctttatatc 
     3781 tgtctaactc gactgtctgg gtttcattga tgtagtcgta tgcatcgtta gttccaaaaa 
     3841 atattgtcat caatttgata ttggtttccg actctaaaat ttttggaaga atttgtctag 
     3901 cgtgctctga gttgtggcca ctgaaaccac ggttaataac atccaatttt cggatataca 
     3961 cattctgtaa tgctggatga aagccatact gggtacaact aaactgggtg atggagtcac 
     4021 cgaacaacac aaatttaccg tattccatga ttgctatggt tgagaatttt ttttttttct 
     4081 tgtcccacgc catttttcaa attatgcagt tgagaatgtt agtttttgtg tacaccccgt 
     4141 tcgctgaata tttcggaata attcaaagat tggggagtgg gggaggcgat agacgaagac 
     4201 acggtataaa aatgggcaaa attttcccca actttttgca gtggtttaac taataatcaa 
     4261 ctacaatgac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta 
     4321 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc 
     4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     4741 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     4981 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     6301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 
     6361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 
     6421 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 
     6481 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 
     6541 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg 
     6601 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga 
     6661 cactata 
//
