LOCUS       pMV261Hyg-GalK-T        7238 bp    DNA     circular     16-JUN-2020
DEFINITION  LMBP 11304.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11304
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 16-JUN-2020
            NGS data implemented
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13388789|
COMMENT     VNTDBDATE|-13388826|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMV261Hyg-GalK-TetRTetO-ISceIM|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMV261Hyg-GalK-TetRTetO-ISceIM" 0 7238 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 7238 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "GalK" 4 0 1 5778 6926 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #1=(CFSignal (CObList) "hygromycin resistance" 4 0 1 3765 4760 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "I-SceI" 4 0 0 2666 3436 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #3=(CFSignal (CObList) "T1 terminator" 43 0 1 3683 3726 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "HA epitope" 22 0 0 2699 2725 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "pMB1" 33 0 0 5469 5747 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "OriM" 33 0 0 358 1642 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "tetracyclin resistance" 4 0 1 1953 2552 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "tetO" 30 0 0 2553 2646 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "LMBP 11304" 2 0 0 0 0 0 (CStringList) 
COMMENT            (CStringList) 1 1 0 0 "") 
COMMENT        #10=(CFSignal (CObList) "Last modified: 16-JUN-2020" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #11=(CFSignal (CObList) "NGS data implemented" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #12=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #13=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "pMV261Hyg-GalK-TetRTetO-ISceIM" 2) 5 "" 0 4) 
COMMENT              #14=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pMV261Hyg-GalK-TetRTetO-ISceIM'" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 11304." 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/16/20 05:44" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 06/16/20 06:21" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7238 bp" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #23=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #24=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #25=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #26=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #27=(CLinePar (CParagraph 0 (15 #9# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 11304") 
COMMENT                     #28=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #29=(CLinePar (CParagraph 0 (15 #10# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 16-JUN-2020") 
COMMENT                     #30=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #31=(CLinePar (CParagraph 0 (15 #11# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "NGS data implemented"))) "Annotations") 
COMMENT              #32=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #33=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #34=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1953 (3 #7# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1953  End: 2552 (Complementary)" 
COMMENT                                        1))) "tetracyclin resistance") 
COMMENT                            #36=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2666 (3 #2# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2666  End: 3436" 1))) "I-SceI") 
COMMENT                            #38=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3765 (3 #1# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3765  End: 4760 (Complementary)" 
COMMENT                                        1))) "hygromycin resistance") 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5778 (3 #0# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5778  End: 6926 (Complementary)" 
COMMENT                                        1))) "GalK"))) "CDS (4 total)") 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2699 (3 #4# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2699  End: 2725" 1))) 
COMMENT                                 "HA epitope"))) "Misc. Marker (1 total)") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2553 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2553  End: 2646" 1))) "tetO"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #49=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 358 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 358   End: 1642" 1))) "OriM") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5469 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5469  End: 5747" 1))) "pMB1"))) 
COMMENT                          "Replication Origin (2 total)") 
COMMENT                     #53=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3683 (3 #3# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3683  End: 3726 (Complementary)" 
COMMENT                                        1))) "T1 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #56=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #57=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #58=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT               "@L bp" 0) 
COMMENT          #59=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #60=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT               0 0) 
COMMENT          #61=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #62=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT               0 0 0) 
COMMENT          #63=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #64=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #65=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #66=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111 
COMMENT               0 1 5 "@N (@S)" 0) 
COMMENT          #67=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1 
COMMENT               2 "?" 0) 
COMMENT          #68=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0) 
COMMENT          #69=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT               "@N (@H%)" 0) 
COMMENT          #70=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT               "@F bp (molecule @L bp)" 0) 
COMMENT          #71=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT               "Fragment of @N" 0) 
COMMENT          #72=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #73=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT               0 0) 
COMMENT          #74=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT               0) #75=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #76=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #77=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #78=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #79=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #80=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #81=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #82=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #83=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 1 
COMMENT               3 "@T @N " 0) 
COMMENT          #84=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #85=(CWidgetStyle "Annotation" 0 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 0 
COMMENT               0) 
COMMENT          #86=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #87=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 16777215 0) 
COMMENT               0 0 0) 
COMMENT          #88=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT               "@N" 0) 
COMMENT          #89=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 65535 
COMMENT               "@N" 0) 
COMMENT          #90=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT               "@N" 0) 
COMMENT          #91=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT               (LOGBRUSH 0 16777215 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #92=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT               1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #93=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 1 
COMMENT               1 "@N" 0) 
COMMENT          #94=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #95=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT               "@N (@S)" 0) 
COMMENT          #96=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT               "@N" 0) 
COMMENT          #97=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT               (LOGBRUSH 0 8388736 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #98=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT               (LOGBRUSH 0 10040064 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #99=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT               (LOGBRUSH 0 10040064 0) 1 
COMMENT               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #100=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #101=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #102=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #103=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #104=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #105=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #106=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #107=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #108=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #109=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #110=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #111=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #112=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #113=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #114=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #115=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 17.3495 -11.0301 91.011 91.011 0.01 10 -1 7238 7238 1 0 0) 
COMMENT        #116=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1768117093 100) 
COMMENT              (CObjectList 
COMMENT               #117=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -936 100) 
COMMENT                     (CObjectList 
COMMENT                      #118=(CAxis 
COMMENT                            (CWideLine (CWidget 0 (0 0) 1 2 0 0 #62# 87068220 0) 
COMMENT                             (LOGPEN 0 2 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27505 
COMMENT                             6.27305 1 0.0214037) 0.0866701) 
COMMENT                      #119=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #59# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 0 
COMMENT                            (LOGFONT 113 42 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.722222 0 "pMV261Hyg-GalK-TetRTetO-ISceIM" 
COMMENT                            "@N" 1 0 0.508571 0 -10 17.2507 1.20865 Nil) 
COMMENT                      #120=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #58# 0 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 165 62 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "7238 bp" "@L bp" 16 
COMMENT                            0 -1.49746 0 -10.8 5.30705 1.79099 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #121=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -866 100) 
COMMENT                     (CObjectList 
COMMENT                      #122=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -487 100) 
COMMENT                            (CObjectList 
COMMENT                             #123=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #0# 0) 1 2 0 0 #60# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.270411 1.26625 1 0.082322) 0.8 1.8 1) 
COMMENT                             #124=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "GalK" "@N" 1 
COMMENT                                   -9.88699 8.71165 0 -10 2.16457 1.13173 #123#) 
COMMENT                             #125=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #1# 0) 1 2 0 0 #60# 1147 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.14769 3.01092 1 0.082322) 0.8 1.8 1) 
COMMENT                             #126=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hygromycin resistance" "@N" 1 -11.8943 
COMMENT                                   -10.454 0 -10 9.3725 1.13173 #125#) 
COMMENT                             #127=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #60# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 3.2952 
COMMENT                                    3.96343 1 0.082322) 0.8 1.8 0) 
COMMENT                             #128=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-SceI" "@N" 1 
COMMENT                                   7.36876 -11.7066 0 -10 2.52717 1.13173 #127#) 
COMMENT                             #129=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #60# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.06136 4.58138 1 0.082322) 0.8 1.8 1) 
COMMENT                             #130=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "tetracyclin resistance" "@N" 1 15.8362 
COMMENT                                   -5.22699 0 -10 8.82311 1.13173 #129#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #131=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -1006 100) 
COMMENT                            (CObjectList 
COMMENT                             #132=(CScratch 
COMMENT                                   (CWidget 0 (3 #4# 0) 1 2 0 0 #99# 0 100) 
COMMENT                                   (LOGPEN 0 10 10040064) 6 1 3.92356 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #133=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "HA epitope" 
COMMENT                                   "@N" 1 11.4972 -9.84338 0 -10 4.69174 1.13173 
COMMENT                                   #132#)) (CObjectList)) 
COMMENT                      #134=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil 1919885412 100) 
COMMENT                            (CObjectList 
COMMENT                             #135=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #102# 0 100) 
COMMENT                                    (LOGPEN 0 2 0) 2 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.835356 3.97989 4.06136 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #136=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "tetO" "@N" 1 
COMMENT                                   10.8381 -8.58451 0 -10 1.83494 1.13173 #135#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #137=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -233 100) 
COMMENT                            (CObjectList 
COMMENT                             #138=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #5# 0) 1 2 0 0 #72# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.29225 1.53406 1 0.082322) 0.8 1.8 0) 
COMMENT                             #139=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1" "@N" 1 
COMMENT                                   -13.1453 1.74233 0 -10 2.52717 1.13173 #138#) 
COMMENT                             #140=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #72# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    4.85006 5.96377 1 0.082322) 0.8 1.8 0) 
COMMENT                             #141=(CLabel (CWidget 0 (0 0) 1 2 0 0 #100# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "OriM" "@N" 1 
COMMENT                                   10.9354 8.17797 0 -10 2.08766 1.13173 #140#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #142=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil 1919885412 100) 
COMMENT                            (CObjectList 
COMMENT                             #143=(CScratch 
COMMENT                                   (CWidget 0 (3 #3# 0) 1 2 0 0 #63# 0 100) 
COMMENT                                   (LOGPEN 0 14 16711680) 8 1 3.08199 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #144=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #110# 8249 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 105 40 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T1 terminator" 
COMMENT                                   "@N" 1 -3.81159 -12.8885 0 -10 5.57076 
COMMENT                                   1.13173 #143#)) (CObjectList))) (CObjectList)) 
COMMENT               #145=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -444 100) 
COMMENT                     (CObjectList 
COMMENT                      #146=(CLabel (CWidget 0 (15 #9# 0) 1 2 0 0 #85# 0 100) 
COMMENT                            (LOGPEN 3467875989 -2147433472 0) -827222377 
COMMENT                            (LOGFONT 143 54 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 11304" 
COMMENT                            "LMBP 11304" 65535 -3.55271e-015 -3.26334 0 -10 
COMMENT                            8.09793 1.57124 Nil) 
COMMENT                      #147=(CLabel (CWidget 0 (15 #10# 0) 1 2 0 0 #85# 0 100) 
COMMENT                            (LOGPEN 3466041017 -2147429888 2147486976) 
COMMENT                            -829057349 
COMMENT                            (LOGFONT 96 36 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 16-JUN-2020" 
COMMENT                            "Last modified: 16-JUN-2020" 65535 -0.296668 
COMMENT                            -4.79063 0 -10 11.625 1.02185 Nil) 
COMMENT                      #148=(CLabel (CWidget 0 (15 #11# 0) 1 2 0 0 #85# 261 100) 
COMMENT                            (LOGPEN 858861936 1731082039 1802659191) 1936158300 
COMMENT                            (LOGFONT 96 36 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "NGS data implemented" 
COMMENT                            "NGS data implemented" 65535 -0.395557 -6.24101 0 
COMMENT                            -10 9.75707 1.02185 Nil)) (CObjectList)) 
COMMENT               #149=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -163 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #150=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -563 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1 
COMMENT       (CStringList) 0 0 (CObList)))
FEATURES             Location/Qualifiers
     CDS             complement(5778..6926)
                     /vntifkey="4"
                     /label=GalK
     CDS             complement(3765..4760)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     CDS             2666..3436
                     /vntifkey="4"
                     /label=I-SceI
     terminator      complement(3683..3726)
                     /vntifkey="43"
                     /label=T1\terminator
     misc_marker     2699..2725
                     /vntifkey="22"
                     /label=HA\epitope
     rep_origin      5469..5747
                     /vntifkey="33"
                     /label=pMB1
     rep_origin      358..1642
                     /vntifkey="33"
                     /label=OriM
     CDS             complement(1953..2552)
                     /vntifkey="4"
                     /label=tetracyclin\resistance
     promoter        2553..2646
                     /vntifkey="30"
                     /label=tetO
BASE COUNT     1583 a      2064 c      2095 g      1496 t 
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcacgcgtga 
      121 gcccaccagc tccgtaagtt cgggcgctgt gtggctcgta cccgcgcatt caggcggcag 
      181 ggggtctaac gggtctaagg cggcgtgtac ggccgccaca gcggctctca gcggcccgga 
      241 aacgtcctcg aaacgacgca tgtgttcctc ctggttggta caggtggttg ggggtgctcg 
      301 gctgtcgctg gtgttccacc accagggctc gacgggagag cgggggagtg tgcagttgtg 
      361 gggtggcccc tcagcgaaat atctgacttg gagctcgtgt cggaccatac accggtgatt 
      421 aatcgtggtc tactaccaag cgtgagccac gtcgccgacg aatttgagca gctctggctg 
      481 ccgtactggc cgctggcaag cgacgatctg ctcgagggga tctaccgcca aagccgcgcg 
      541 tcggccctag gccgccggta catcgaggcg aacccaacag cgctggcaaa cctgctggtc 
      601 gtggacgtag accatccaga cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg 
      661 ctgcccaacg cgatcgtggg caatcgcgcc aacggccacg cacacgcagt gtgggcactc 
      721 aacgcccctg ttccacgcac cgaatacgcg cggcgtaagc cgctcgcata catggcggcg 
      781 tgcgccgaag gccttcggcg cgccgtcgat ggcgaccgca gttactcagg cctcatgacc 
      841 aaaaaccccg gccacatcgc ctgggaaacg gaatggctcc actcagatct ctacacactc 
      901 agccacatcg aggccgagct cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc 
      961 acgtacaaag cggctccgac gccgctaggg cggaattgcg cactgttcga ttccgtcagg 
     1021 ttgtgggcct atcgtcccgc cctcatgcgg atctacctgc cgacccggaa cgtggacgga 
     1081 ctcggccgcg cgatctatgc cgagtgccac gcgcgaaacg ccgaatttcc gtgcaacgac 
     1141 gtgtgtcccg gaccgctacc ggacagcgag gtccgcgcca tcgccaacag catttggcgt 
     1201 tggatcacaa ccaagtcgcg catttgggcg gacgggatcg tggtctacga ggccacactc 
     1261 agtgcgcgcc agtcggccat ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca 
     1321 gttgcgcggc gcgcaaagtc cgcgtcagcc atgcatggag gcattgctat gagcgacggc 
     1381 tacagcgacg gctacaaccg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc 
     1441 gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa 
     1501 cgcagcgagt ggctcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac 
     1561 gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt 
     1621 aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag 
     1681 gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg 
     1741 ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct 
     1801 agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgccc gttttgaata 
     1861 ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat 
     1921 atcgtgcgtt taatactgtt taaacctcta gatcacgatt cgctcgaggt caatgacgct 
     1981 gtgcgggagt gagagaatcc gtcgatcagt gctgtcaggc cgaaatcgaa ttcccggtcg 
     2041 cgttcaggaa cgctcgcatt gccgacctct gcggtttgct cctccagtac gacaccgacg 
     2101 gtgtatcggc tgaccgcgat gagcatgctg atggctgtct gttcatcgaa tccctgagac 
     2161 acgagaagcc cgacttgctg accggaagcc tcagctcctt cgtcggtgtc gtcgccggat 
     2221 ggtctgcggt ggtgctcagc gtggagacgc gcaccgtcac ggaccgcgtg tagtgcgtgg 
     2281 cggaaacttc ttgcgtttcg caagagaaat gcgtcccatt tctcgtcgga ctcggggaag 
     2341 gaagcgtgat gctctcggtc aagcacgtcg ctcgccagcg ctgcgaggag ttcggccttc 
     2401 gtgcggaagt gccagtagag gccgggctgc tgtacctgta agtgagccgc cagcgcgcga 
     2461 gtggtgaagc catcgagccc agtctcgtcg agcacctgcc gggccccgag caacacggac 
     2521 gtgcggtcga gacgcttccg gtggtgagtc atagttgcac tttatcatcg ataactttat 
     2581 cttagataaa gtgactgctc gctactctca tctgactgct cgctactctc atcgtggaat 
     2641 cctgacagga tccggaggaa atgttatgca gaaaggaggc catatgggac gatttatgta 
     2701 cccatacgat gttcctgact atgcgggtat gaaaaacatc aaaaaaaacc aggtaatgaa 
     2761 cctgggtccg aactctaaac tgctgaaaga atacaaatcc cagctgatcg aactgaacat 
     2821 cgaacagttc gaagcaggta tcggtctgat cctgggtgat gcttacatcc gttctcgtga 
     2881 tgaaggtaaa acctactgta tgcagttcga gtggaaaaac aaagcataca tggaccacgt 
     2941 atgtctgctg tacgatcagt gggtactgtc cccgccgcac aaaaaagaac gtgttaacca 
     3001 cctgggtaac ctggtaatca cctggggcgc ccagactttc aaacaccaag ctttcaacaa 
     3061 actggctaac ctgttcatcg ttaacaacaa aaaaaccatc ccgaacaacc tggttgaaaa 
     3121 ctacctgacc ccgatgtctc tggcatactg gttcatggat gatggtggta aatgggatta 
     3181 caacaaaaac tctaccaaca aatcgatcgt actgaacacc cagtctttca ctttcgaaga 
     3241 agtagaatac ctggttaagg gtctgcgtaa caaattccaa ctgaactgtt acgtaaaaat 
     3301 caacaaaaac aaaccgatca tctacatcga ttctatgtct tacctgatct tctacaacct 
     3361 gatcaaaccg tacctgatcc cgcagatgat gtacaaactg ccgaacacta tctcctccga 
     3421 aactttcctg aaataataag tcgatcgagg aattcactcc tcaggtgcag gctgcctatc 
     3481 agaaggtggt ggctggtgtg gaaacgcggc cgcacgcgtt gcgctcggtc gttcggctgc 
     3541 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     3601 acgcaggaaa gaacatggat gaattcgaag cttatcgatg tcgacgtagt taactagcgt 
     3661 acgatcgact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     3721 ttttatgcca tcatggccgc ggtgatcagc tagagggggc gtcaggcgcc gggggcggtg 
     3781 tccggcggcc cccagaggaa ctgcgccagt tcctccggat cggtgaagcc ggagagatcc 
     3841 agcggggtct cctcgaacac ctcgaagtcg tgcaggaagg tgaaggcgag cagttcgcgg 
     3901 gcgaagtcct cggtccgctt ccactgcgcc ccgtcgagca gcgcggccag gatctcgcgg 
     3961 tcgccccgga aggcgttgag atgcagttgc accaggctgt agcgggagtc tcccgcatag 
     4021 acgtcggtga agtcgacgat cccggtgacc tcggtcgcgg ccaggtccac gaagatgttg 
     4081 gtcccgtgca ggtcgccgtg gacgaaccgg ggttcgcggc cggccagcag cgtgtccacg 
     4141 tccggcagcc agtcctccag gcggtccagc agccggggcg agaggtagcc ccacccgcgg 
     4201 tggtcctcga cggtcgccgc gcggcgttcc cgcagcagtt ccgggaagac ctcggaatgg 
     4261 ggggtgagca cggtgttccc ggtcagcggc accctgtgca gccggccgag cacccggccg 
     4321 agttcgcggg ccagggcgag cagcgcgttc cggtcggtcg tgccgtccat cgcggaccgc 
     4381 caggtggtgc cggtcatccg gctcatcacc aggtagggcc acggccaggc tccggtgccg 
     4441 ggccgcagct cgccgcggcc gaggaggcgg ggcaccggca ccggggcgtc cgccaggacc 
     4501 gcgtacgcct ccgactccga cgcgaggctc tccggaccgc accagtgctc gccgaacagc 
     4561 ttgatcaccg ggccgggctc gccgaccagt acggggttgg tgctctcgcc gggcacccgc 
     4621 agcaccggcg gcaccggcag cccgagctcc tccagggctc ggcgggccag cggctcccag 
     4681 aattcctggt cgttccgcag gctcgcgtag gaatcatccg aatcaatacg gtcgagaagt 
     4741 aacagggatt cttgtgtcac agcggacctc tattcacagg gtacgggccg gcttaattcc 
     4801 gcacggccgg tcgcgacacg gcctgtccgc accgcggatc aggcgttgac gatgacgggc 
     4861 tggtcggcca cgtcggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     4921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     5041 aatctcatgc tctagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     5101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     5161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     5221 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 
     5281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     5341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     5401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     5461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga 
     5521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     5581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     5641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     5701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggtg gacctcgacg 
     5761 acctgcagga gtttcgttca gcactgtcct gctccttgtg atggtttaca aacgtaaaaa 
     5821 gtctctttaa tacctgtttt tgcttcatat tgttcagcga cagcttgctg tacggcaggc 
     5881 accagctctt ccgggatcag cgcgacgata cagccgccaa atccgccgcc ggtcatgcgt 
     5941 acgccacctt tgtcgccaat cacagctttg acgatttcta ccagagtgtc aatttgcggc 
     6001 acggtgattt cgaaatcatc gcgcatagag gcatgagact ccgccatcaa ctcgcccata 
     6061 cgtttcaggt cgccttgctc cagcgcgctg gcagcttcaa cggtgcgggc gttttcagtc 
     6121 agtatatgac gcacgcgttt tgccacgatc gggtccagtt catgcgcaac agcgttgaac 
     6181 tcttcaatgg tgacatcacg cagggctggc tgctggaaga aacgcgcacc ggtttcgcac 
     6241 tgttcacgac gggtgttgta ttcgctgcca accagggtac gtttgaagtt actgttgatg 
     6301 atgacgacag ccacaccttt gggcatggaa actgctttgg tccccagtga gcggcaatcg 
     6361 atcagcaagg catgatcttt cttgccgagc gcggaaatta gctgatccat gatcccgcag 
     6421 ttacagccta caaactggtt ttctgcttcc tgaccgttaa gcgcgatttg tgcgccgtcc 
     6481 agcggcagat gataaagctg ctgcaatacg gttccgaccg cgacttccag tgaagcggaa 
     6541 gaacttaacc cggcaccctg cggcacattg ccgctgatca ccatgtccac gccgccgaag 
     6601 ctgttgttac gcagttgcag atgtttcacc acgccacgaa cgtagttagc ccattgatag 
     6661 ttttcatgtg cgacaatggg cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg 
     6721 gctgccatca cgcgaacttt acggtcatcg cgtggtgcac aactgatcac ggtttgataa 
     6781 tcaatcgcgc agggcagaac gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc 
     6841 acgcggccag gcgcctgaat ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga 
     6901 gattgtgttt tttctttcag actcatttct tacactccgg aagatctgtt cctgtgttat 
     6961 cggatcaagc ttggtaccgc atgcgaattg tgagcgctca caattccaca cattatacga 
     7021 gccggaagca taaagtgtca agcctggggt ctggatccat ggatatctag ttctagccac 
     7081 ctgacgtcgg ggggggggga aagccacgtt gtgtctcaaa atctctgatg ttacattgca 
     7141 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 
     7201 aggggtgtta tgagccatat tcaacgggaa acgtcttg 
//
