LOCUS       pOSV802                 5681 bp    DNA     circular SYN  5-MAY-2020
DEFINITION  LMBP 11370.
ACCESSION   pOSV801
SOURCE      Synthetic construct (unknown).
  ORGANISM  Synthetic construct
            Cloning vector.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Aubry, C. Pernodet, J-L. and Lautru, S.
  TITLE     A set of modular and integrative vectors for synthetic biology in Streptomyces  
  JOURNAL   Unpublished.
COMMENT     Accession number: LMBP 11370
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-MAY-2020
            NGS data implemented
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13327855|
COMMENT     VNTDBDATE|-13327856|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pOSV802|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pOSV801\">pOSV801</a>" 
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FEATURES             Location/Qualifiers
     promoter        23..133
                     /regulatory class="promoter"
                     /vntifkey="30"
                     /label=ndh\promoter
                     /note="Unknown feature type:regulatory  p(ndh)* promoter"
     gene            160..825
                     /gene="amilCP"
                     /product="blue chromoprotein AmilCP"
                     /vntifkey="60"
     terminator      925..965
                     /regulatory class="terminator"
                     /vntifkey="43"
                     /label=T7Te\terminator
                     /note="Unknown feature type:regulatory  T7TE"
     gene            complement(3834..4610)
                     /gene="aac(3)IV"
                     /product="Apramycin resistance"
                     /vntifkey="60"
     rep_origin      complement(4927..5426)
                     /vntifkey="33"
                     /label=p15A\ori
     terminator      complement(848..891)
                     /vntifkey="43"
                     /label=T1\terminator
     rep_origin      3328..3438
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     CDS             complement(3834..4610)
                     /vntifkey="4"
                     /label=apramycin\resistance
     CDS             160..825
                     /vntifkey="4"
                     /label=AmCP580
     misc_feature    5648..5681
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3093..3126
                     /vntifkey="21"
                     /label=FRT
     CDS             complement(1007..2848)
                     /vntifkey="4"
                     /label=phiC31\int
     attenuator      2865..2903
                     /vntifkey="0"
                     /label=attP
BASE COUNT     1218 a      1654 c      1562 g      1247 t 
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca 
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc 
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa 
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc 
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc 
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca 
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat 
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc 
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc 
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt 
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt 
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg 
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt 
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag 
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 
      961 ttatatacta gtagcggccg cttaagcagg cttcccgggt gtctcgctac gccgctacgt 
     1021 cttccgtgcc gtcctgggcg tcgtcttcgt cgtcgtcggt cggcggcttc gcccacgtga 
     1081 tcgaagcgcg cttctcgatg ggcgttccct gccccctgcc cgtagtcgac ttcgtgacaa 
     1141 cgatcttgtc tacgaagagc ccgacgaaca cgcgcttgtc gtctactgac gcgcgccccc 
     1201 accacgactt agggccggtc gggtcagcgt cggcgtcttc ggggaaccat tggtcaaggg 
     1261 gaagcttcgg ggcttcggcg gcttcaagtt cggcaagccg ctcttccgcc ccttgctgcc 
     1321 ggagcgtcag cgctgcctgt tgcttccgga agtgcttcct gccaacgggt ccgtcgtacg 
     1381 cgcctgccgc gcggtcttcg tacagctctt caagggcgtt cagggcgtcg gcgcgctccg 
     1441 caacaaggtt cgcccgttcg ccgctcttct caggcgcctc agtgagcttg ccgaagcgtc 
     1501 gggcggcttc ccacagaagc gccaacgtct cttcgtcgcc ttcggcgtgc ctgatcttgt 
     1561 tgaagatgcg ttccgcaacg aacttgtcga gtgccgccat gctgacgttg cacgtgcctt 
     1621 cgtgctgccc aggtgcggac gggtcgacca ccttccggcg acggcagcgg taagagtcct 
     1681 tgatcgattc ttccccgcgc ttcgaagtca tgacggcgcc acactcgcag tacagcttgt 
     1741 ccatggcgga cagaatggct tgcccccggg aaagcccctt gccgcgcccc ctgccgtcca 
     1801 accacgcctg aagctcatac cactcagcgg gctcgatgat cggtccgcaa tcaagctcga 
     1861 ccggccggag cgtgatcggg tcgcgctgaa tgcggtaacc ctcaatcttc gtggtcggcg 
     1921 tgccgtccgg cttcttcttg tagatcacct cagcggcgaa gcccgcaata cgcgggtccc 
     1981 gaaggattcg cataacggtt gccgggtccc aggcgcttga agcggtcttc ttcccaatcg 
     2041 tctcgccccg ggtcggcacg gcgtcagcgt ccatgcgctt acaaagcccc gtgatgctgc 
     2101 ccgggtgaat ggcggcttga ctgcccggct tgaagggaag gtgtttgtgc gtcttgatct 
     2161 cacgccacca ccaccggatt acgtcgggct cgaactcgaa gggtccggta aggggagtgg 
     2221 tcgagtgcgc aagcttgttg atgacgacat tgaccattcg gccgttgcgc gtgatctcct 
     2281 tcgtctccga aacaagctcg aagccgtaag gcgccttccc gccgacgtac ccgcccaatt 
     2341 cgcgctgaag gttcttcgtg tcgagaatct tcgccgactt cagcgaagat tctttgtgcg 
     2401 acgcgtcgag ccgcataatc aggtgaatca ggtccatgac gtttccctgc cggaagacgc 
     2461 cttcctgagt ggaaacaatc gtcacgccca gggcgagcaa ttccgagaca atcggaatcg 
     2521 cgtccatgac cttcaggcgc gagaagcgcg acacgtcata gacaatgatc atgttgagcc 
     2581 gcccggcgcg gcattcgttc aggatgcgtt cgaactccgg gcgctccgcc gtcccgaacg 
     2641 ccgacgtgcc cggcgcttcg ctgaaatgcc cgacgaacct gaaccggccc ccgtcgcgct 
     2701 cgacttcgcg ctgaaggtcg gccgccttgt cttcgttggc gctacgctgt gtcgctgggc 
     2761 ttgctgcgct cgaattttcg cgctcgcgcg actgacggtc gtaagcaccc gcgtacgtgt 
     2821 ccaccccggt cacaacccct tgtgtcatgt cggcgaccct acgcccccaa ctgagagaac 
     2881 tcaaaggtta ccccagttgg ggcactactc ccgaaaaccg cttctgacct gggaaaacgt 
     2941 gaagccccgg ggcatccgct gagggttgcc gccggggctt cggtgtgtcc gtcagtacgg 
     3001 gccatagagg ggcgtcgtgt aacccaccca gggcgattgc tccggcacgg ggaagccgcg 
     3061 ccacgccttc gggacgtctg gaatcctgca gggaagttcc tattctctag aaagtatagg 
     3121 aacttcgtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga 
     3181 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca 
     3241 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt 
     3301 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag 
     3361 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac 
     3421 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct 
     3481 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat 
     3541 gaccccgaag cagggttatg cagcggaaaa gggtaccacg tcgcggtgag ttcaggcttt 
     3601 ttcatatctc attgcccccg gacgagcgtc tgctccgcca ttcgccgtcc gccgtgccaa 
     3661 tcggatcagc cgtccaaatg cgggattttc gttagtcgga ggccaaacgg cattgagcgt 
     3721 cagcatatca tcagcgagct gaagaaagac aatccccgat ccgctccacg tgttgcccca 
     3781 gcaatcagcg cgaccttgcc cctccaacgt catctcgttc tccgctcatg agctcagcca 
     3841 atcgactggc gagcggcatc gcattcttcg catcccgcct ctggcggatg caggaagatc 
     3901 aacggatctc ggcccagttg acccagggct gtcgccacaa tgtcgcggga gcggatcaac 
     3961 cgagcaaagg catgaccgac tggaccttcc ttctgaaggc tcttctcctt gagccacctg 
     4021 tccgccaagg caaagcgctc acagcagtgg tcattctcga gataatcgac gcgtaccaac 
     4081 ttgccatcct gaagaatggt gcagtgtctc ggcaccccat agggaacctt tgccatcaac 
     4141 tcggcaagat gcagcgtcgt gttggcatcg tgtcccacgc cgaggagaag tacctgccca 
     4201 tcgagttcat ggacacgggc gaccgggctt gcaggcgagt gaggtggcag gggcaatgga 
     4261 tcagagatga tctgctctgc ctgtggcccc gctgccgcaa aggcaaatgg atgggcgctg 
     4321 cgctttacat ttggcaggcg ccagaatgtg tcagagacaa ctccaaggtc cggtgtaacg 
     4381 ggcgacgtgg caggatcgaa cggctcgtcg tccagacctg accacgaggg catgacgagc 
     4441 gtccctcccg gacccagcgc agcacgcagg gcctcgatca gtccaagtgg cccatcttcg 
     4501 aggggccgga cgctacggaa ggagctgtgg accagcagca caccgccggg ggtaacccca 
     4561 aggttgagaa gctgaccgat gagctcggct tttcgccatt cgtattgcac gacattgcac 
     4621 tccaccgctg atgacatcag tcgatcatag cacgatcaac ggcactgttg caaatagtcg 
     4681 gtggtgataa acttatcatc cccttttgct gatggagctg cacatgaacc ggatccccta 
     4741 gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat 
     4801 gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt 
     4861 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg 
     4921 cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga 
     4981 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat 
     5041 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     5101 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc 
     5161 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc 
     5221 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg 
     5281 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca 
     5341 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa 
     5401 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta 
     5461 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag 
     5521 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc 
     5581 tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat 
     5641 aagttgtgaa gttcctattc tctagaaagt ataggaactt c 
//
