LOCUS       pOSV804                 5927 bp    DNA     circular SYN  5-MAY-2020
DEFINITION  LMBP 11372.
ACCESSION   pOSV801
SOURCE      Synthetic construct (unknown).
  ORGANISM  Synthetic construct
            Cloning vector.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Aubry, C. Pernodet, J-L. and Lautru, S.
  TITLE     A set of modular and integrative vectors for synthetic biology in Streptomyces  
  JOURNAL   Unpublished.
COMMENT     Accession number: LMBP 11372
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-MAY-2020
            NGS data implemented
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13327886|
COMMENT     VNTDBDATE|-13327886|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pOSV804|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pOSV801\">pOSV801</a>" 
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FEATURES             Location/Qualifiers
     promoter        23..133
                     /regulatory class="promoter"
                     /vntifkey="30"
                     /label=ndh\promoter
                     /note="Unknown feature type:regulatory  p(ndh)* promoter"
     gene            160..825
                     /gene="amilCP"
                     /product="blue chromoprotein AmilCP"
                     /vntifkey="60"
     terminator      925..965
                     /regulatory class="terminator"
                     /vntifkey="43"
                     /label=T7Te\terminator
                     /note="Unknown feature type:regulatory  T7TE"
     gene            complement(4080..4856)
                     /gene="aac(3)IV"
                     /product="Apramycin resistance"
                     /vntifkey="60"
     rep_origin      complement(5173..5672)
                     /vntifkey="33"
                     /label=p15A\ori
     terminator      complement(848..891)
                     /vntifkey="43"
                     /label=T1\terminator
     rep_origin      3574..3684
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     CDS             complement(4080..4856)
                     /vntifkey="4"
                     /label=apramycin\resistance
     CDS             160..825
                     /vntifkey="4"
                     /label=AmCP580
     misc_feature    5894..5927
                     /vntifkey="21"
                     /label=FRT
     misc_feature    3339..3372
                     /vntifkey="21"
                     /label=FRT
     attenuator      complement(1198..1242)
                     /vntifkey="0"
                     /label=attP
     CDS             complement(1400..2683)
                     /vntifkey="4"
                     /label=VWB\int
BASE COUNT     1231 a      1714 c      1707 g      1275 t 
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca 
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc 
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa 
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc 
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc 
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca 
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat 
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc 
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc 
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt 
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt 
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg 
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt 
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag 
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 
      961 ttatatacta gtagcggccg cttaaggtcg acccgtctga cgcgtgtggg gagcacgccg 
     1021 gcccgctcgg tagacagcac tctacgcatt gtggctgctg tgcacatcac tctttcgagc 
     1081 gacgtgatcc gcaggattcc gtggggatgt ggcatctatg tggcatccgg gtacgacaac 
     1141 ggccccccgt tcgaatggaa cggggggccg ttgactccct gacctgcgat gcaggttgtg 
     1201 cccccggcag gattcgaacc tgcgacaccc gctttaggag agaacgtaaa gcgatcatga 
     1261 gcctcagtga gtctgagtga gtctcagtga gcctgtcgcc ataggggctt cacctgcata 
     1321 aatgagtttc agtgagtctc ggtgagtctc agtgaggggg tgttgtggca tccctgtggc 
     1381 atctgcgtgg catccggcgt cacaccgccc gcagcgttcg aacaccctcg gtctcgggag 
     1441 ccatcgccgc ccgcaccttc gccgccacgt cggccggcga atgctggtac aaccacgtca 
     1501 ccttcgaccc tcgctcgtga cccatcacgg tctgcgtgtc cttctcgtca atgcccacat 
     1561 ccttgagcca cgtggcgaac acgtggcgaa ggtcgtgcac ccgcggccac cactccatgc 
     1621 ggccggtgtc ccggttcttc acctcgcggg cgaggcctgc agccttgatt gcaggtatcc 
     1681 atgtgcgccg gaagttgtgc cgggtcagga cgccgccctg tgggccgcgg aacacgagct 
     1741 catccggcgc gaggtcgtac gggtcctcgc caatcggggt gatcgtggga gtcggccgcc 
     1801 accgggtgac catggtgcgg atcgcgtcct cggcctgcgg cgtgagcggg accgtgcgga 
     1861 acccggcgtt gctcttcggc gcaggcttcc ggaacagctt gccttcgtcg tcactgagca 
     1921 cttccttcac cttgaggtgg tgggcgtcca ggtcgacgtt ctcccagcgc agcgccgtcg 
     1981 cctcgcccca ccggagaccg gtgtgttcaa ggaagacgac gagcggccgg tagtacatcg 
     2041 gcaggtgact gacgatcagc gcgcactgcg cgcggtcggg cggcatcagt tcgtccggat 
     2101 gcttcgccgg cgcctcgcca atgtccaggt cggccgccgg gttgaacggg atacgccggc 
     2161 cgtccttgac ggcggcccgg agcatcgagt tcagcacctc atgaaccttc ttccgggtgt 
     2221 ggtagccctt cacctccttc gtgatccacg cctgcagctc gatgtactcc aagtcgcaga 
     2281 gacgccactg cccccacttc ggctcgatgt gcgcggccca gttcgaccgc ttccggttgg 
     2341 cggtcgtgac tgctcggtcc ggctgcgcct cccaccacag tttccaccac tgggacaggg 
     2401 tgatctcgcc acgcttcggg tcggcgtacg tccgttcgcg gacctgcgtg cggatcttgt 
     2461 cgaggtgcgc ctcggctgcc ttcttaccgc cgtccttgat cgcaaagcat ttctgcttct 
     2521 ggttgccggc cgggtcgcgg taacgcgcct gccacgagcc ggtgcagtcg cgtcggcggt 
     2581 tccgctctcc gtacttctcg ggcgggtact tctccatgca cagctggcag ccgcagctct 
     2641 tcgccctcaa ctgccgtggg ttgttcgtcg ctctaggcgc catgggtcac ctgtccgctc 
     2701 ctccgctgtt cgggaacgcg gggcaggatg tgtacggctg ccccacacca gcagactgca 
     2761 ccgtccgggg gctgctctac agccagttct tcaaggacgg ctcgaagggc tacgagggaa 
     2821 gcatcccggg ggagggtggc aggcagggtg accgtgtttc cagcggcgtc gtacggccgg 
     2881 aagttgagcg ggtcgagagg cgcgaagcgg acacgaatgc acatgggtat cccccgggca 
     2941 ggcaggcgac gtttgaccgt cggccatggg ggagggcagg ccgctcgacc gaccgtaccc 
     3001 ccttgtaggt gaatatgcga ccactctgcg tgcaccaagt gcccgaggaa tcacggggag 
     3061 tggtggagtc cattttcgtt agacccgcga ggcaggccga cggttgtacg ggcgtcggct 
     3121 actgcttgtt ggactcgttc agcgcgctca tctcgatcag cttggaacgc tgctgctcct 
     3181 cggtgagacc acggaacagg gtgagcaggc gttcctcggc gtcctcgtcg agcgggcccg 
     3241 gcgcggcccg gcgggccgcg gcgaaaatgc gctcttcggt gaacttcggg tacgcctcat 
     3301 ggagggcgcg aagctttcgc gagctcgaga cctgcaggga agttcctatt ctctagaaag 
     3361 tataggaact tcgtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta 
     3421 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg 
     3481 aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat 
     3541 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc 
     3601 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg 
     3661 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg 
     3721 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc 
     3781 tataatgacc ccgaagcagg gttatgcagc ggaaaagggt accacgtcgc ggtgagttca 
     3841 ggctttttca tatctcattg cccccggacg agcgtctgct ccgccattcg ccgtccgccg 
     3901 tgccaatcgg atcagccgtc caaatgcggg attttcgtta gtcggaggcc aaacggcatt 
     3961 gagcgtcagc atatcatcag cgagctgaag aaagacaatc cccgatccgc tccacgtgtt 
     4021 gccccagcaa tcagcgcgac cttgcccctc caacgtcatc tcgttctccg ctcatgagct 
     4081 cagccaatcg actggcgagc ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg 
     4141 aagatcaacg gatctcggcc cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg 
     4201 atcaaccgag caaaggcatg accgactgga ccttccttct gaaggctctt ctccttgagc 
     4261 cacctgtccg ccaaggcaaa gcgctcacag cagtggtcat tctcgagata atcgacgcgt 
     4321 accaacttgc catcctgaag aatggtgcag tgtctcggca ccccataggg aacctttgcc 
     4381 atcaactcgg caagatgcag cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc 
     4441 tgcccatcga gttcatggac acgggcgacc gggcttgcag gcgagtgagg tggcaggggc 
     4501 aatggatcag agatgatctg ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg 
     4561 gcgctgcgct ttacatttgg caggcgccag aatgtgtcag agacaactcc aaggtccggt 
     4621 gtaacgggcg acgtggcagg atcgaacggc tcgtcgtcca gacctgacca cgagggcatg 
     4681 acgagcgtcc ctcccggacc cagcgcagca cgcagggcct cgatcagtcc aagtggccca 
     4741 tcttcgaggg gccggacgct acggaaggag ctgtggacca gcagcacacc gccgggggta 
     4801 accccaaggt tgagaagctg accgatgagc tcggcttttc gccattcgta ttgcacgaca 
     4861 ttgcactcca ccgctgatga catcagtcga tcatagcacg atcaacggca ctgttgcaaa 
     4921 tagtcggtgg tgataaactt atcatcccct tttgctgatg gagctgcaca tgaaccggat 
     4981 cccctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg 
     5041 cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga 
     5101 tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg 
     5161 aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg 
     5221 aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca 
     5281 cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc 
     5341 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt 
     5401 cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg 
     5461 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct 
     5521 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag 
     5581 cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa 
     5641 actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag 
     5701 ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag 
     5761 caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa 
     5821 tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata 
     5881 cgatataagt tgtgaagttc ctattctcta gaaagtatag gaacttc 
//
