LOCUS       pOSV811                 5242 bp    DNA     circular SYN  5-MAY-2020
DEFINITION  LMBP 11379.
ACCESSION   pOSV801
SOURCE      Synthetic construct (unknown).
  ORGANISM  Synthetic construct
            Cloning vector.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Aubry, C. Pernodet, J-L. and Lautru, S.
  TITLE     A set of modular and integrative vectors for synthetic biology in Streptomyces  
  JOURNAL   Unpublished.
COMMENT     Accession number: LMBP 11379
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-MAY-2020
            NGS data implemented
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13327970|
COMMENT     VNTDBDATE|-13327970|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pOSV811|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pOSV801\">pOSV801</a>" 
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FEATURES             Location/Qualifiers
     promoter        23..133
                     /regulatory class="promoter"
                     /vntifkey="30"
                     /label=ndh\promoter
                     /note="Unknown feature type:regulatory  p(ndh)* promoter"
     gene            160..825
                     /gene="amilCP"
                     /product="blue chromoprotein AmilCP"
                     /vntifkey="60"
     terminator      925..965
                     /regulatory class="terminator"
                     /vntifkey="43"
                     /label=T7Te\terminator
                     /note="Unknown feature type:regulatory  T7TE"
     terminator      complement(848..891)
                     /vntifkey="43"
                     /label=T1\terminator
     CDS             160..825
                     /vntifkey="4"
                     /label=AmCP580
     misc_feature    5209..5242
                     /vntifkey="21"
                     /label=FRT
     misc_feature    2632..2665
                     /vntifkey="21"
                     /label=FRT
     attenuator      complement(1157..1215)
                     /vntifkey="0"
                     /label=attP
     CDS             complement(1276..2442)
                     /vntifkey="4"
                     /label=pSAM2\int
     rep_origin      complement(4488..4987)
                     /vntifkey="33"
                     /label=p15A\ori
     rep_origin      2867..2977
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     CDS             complement(3373..4167)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
BASE COUNT     1169 a      1514 c      1427 g      1132 t 
ORIGIN
        1 atgcatgcgg ccgctgctag cgttttttga tctcaatcaa taaagtcgcc tatcttttca 
       61 gcaacaaaac ttgattaaca tcaattttgg tatgaccaat gcaccattca tgttattctc 
      121 aatagcgaag aactactaga gaaagaggag aaatactaga tgagtgtgat cgctaaacaa 
      181 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc 
      241 gatggaaaag gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc 
      301 ggacctctgc catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca 
      361 ttcaccaagt accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat 
      421 acatgggaga ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc 
      481 agcatccaag gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc 
      541 aatggacctg tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt 
      601 gcacgagatg gaatgctgct aggaaacaac tttatggctc tgaagttaga aggaggcggt 
      661 cactatttgt gtgaatttaa aactacttac aaggcaaaga agcctgtgaa gatgccaggg 
      721 tatcactatg ttgaccgcaa actggatgta accaatcaca acaaggatta cacttcggtt 
      781 gagcagtgtg aaatttccat tgcacgcaaa cctgtggtcg cctaataata ctagagccag 
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 
      961 ttatatacta gtagcggccg cttaaggtca gtcatgcggg caacttagcc gagccctacg 
     1021 acaccgcccg tgggaaggtg agtgtctaac tgcgtgacaa cgccagcgca cagcggcgga 
     1081 caaccgcgag cacccatgga ctggcgccgc aggtgagaag cacactggcc caaggtcgag 
     1141 cgcccaccca agttgcttcg ggacgaagag gtcgtgggtt caaatcccgc caccccgaca 
     1201 gagaaacacc aggtgaggca gaccgtaacg ttacggtctg cctcacctgt tttctgtgcg 
     1261 tgtctatctg cgtgactatc gcgccggacc ccgcttgaag atgccgtcca tgaccacagc 
     1321 gccggtctgg atgacgggcc ggatctgctt ccggtagacc tcctcagtca cggccgtacc 
     1381 ggagtgtccg acgagccggg agatctcctc cagcgggacg ccgcggtcgg acagcaggga 
     1441 cacgaagctg tgcctcagct ccctcggtgt ccactcgtcg gcgttgatcc cgttggcatc 
     1501 cttgagcgcc tggcggaagg cgcgccggac gttagtcgcg tcgagcggct tgccaacggc 
     1561 cgacgagaag accaggccgt gttcctccca cttgtcaccg gcggcgagcc gttcccagcc 
     1621 ctggtcctca aagtgctgcc agaggacctc cacgcaacgc gccggcaggg cgagcgttcg 
     1681 ccgagacttc cgggttttcg tgtccccacc gcgccggacc gagcgccaga cggcgatgtg 
     1741 cggaggctgc ggcggctcaa cgtccggact tcccttgagg aagacgtggt cccaggtcag 
     1801 cgcccgcagc tcctcggtgc gcgcaccggt cagcagggcg acgacgatgt aggcgtgcat 
     1861 cgacgtgccc tcggcagcat tcagcaccgc ctcggcctgg gcgaaggtga gcgccttgga 
     1921 cggccggcca ggctggccct ggggcacaga gcacagctcc accacgttgc gcttcacctt 
     1981 gtcacgcgcc atggcccgct tgaccgcccg gttcaggcag gagtggaccg cctgaaggct 
     2041 gcgcgtgctc agagtctgag ccttggcggc cagccagcgg tcgacgtcct ctgcgctgag 
     2101 gtcacgcagc ttccgggcac ccaaacccgg tatgacgtgc ttctggctta ggtgggtgca 
     2161 gttctcgacg gtgcgctggt cacggccagc gagaccgtag gcaagccagt cgttcaccgc 
     2221 gtcggcgacg gtgtaccccg tgggtgcgat cgcgagaccg tcttcgtggt cacgcagaac 
     2281 ctctttgagc ttgttcttgg cctccgtctt ggtcttgcca ctcccccgct tgacgatccg 
     2341 cttaccgctc ggatcgaagc cgaggttcgc cgtggcgatc cagcgctgtc tcttctcgtc 
     2401 ccagtggagg ccgccgtcac cccggctacg tcgcttggcc atcagatggc ctctcccaac 
     2461 tggtcctgaa tgaagtcgcg tacccgggga ttcccggtta ccgagctcga attccatggt 
     2521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggatga 
     2581 ttaattgtca acagctcatt tcagaatatt tgccagaacc gttcctgcag ggaagttcct 
     2641 attctctaga aagtatagga acttcgtcca cgacgcccgt gattttgtag ccctggccga 
     2701 cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc 
     2761 ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg 
     2821 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca 
     2881 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc 
     2941 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag 
     3001 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata 
     3061 ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag ggtaccacgt 
     3121 cgcggtgagt tcaggctttt tcatatctca ttgcccccgg acgagcgtct gctccgccat 
     3181 tcgccgtccg ccgtgccaat cggatcagcc gtccaaatgc gggattttcg ttagtcggag 
     3241 gccaaacggc attgagcgtc agcatatcat cagcgagctg aagaaagaca atccccgatc 
     3301 cgctccacgt gttgccccag caatcagcgc gaccttgccc ctccaacgtc atctcgttct 
     3361 ccgctcatga gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc 
     3421 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc 
     3481 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc 
     3541 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc 
     3601 gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag 
     3661 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc 
     3721 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 
     3781 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 
     3841 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 
     3901 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 
     3961 ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt 
     4021 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 
     4081 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 
     4141 tgcgtgcaat ccatcttgtt caatcatgcg agacattgca ctccaccgct gatgacatca 
     4201 gtcgatcata gcacgatcaa cggcactgtt gcaaatagtc ggtggtgata aacttatcat 
     4261 ccccttttgc tgatggagct gcacatgaac cggatcccct agcggagtgt atactggctt 
     4321 actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc 
     4381 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga 
     4441 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga 
     4501 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg 
     4561 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg 
     4621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg 
     4681 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg 
     4741 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat 
     4801 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     4861 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg 
     4921 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact 
     4981 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa 
     5041 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac 
     5101 gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta 
     5161 tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtga agttcctatt 
     5221 ctctagaaag tataggaact tc 
//
