LOCUS       pECFP-p65               6345 bp    DNA     circular UNA 12-MAY-2020
DEFINITION  LMBP 11490.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11490
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-MAY-2020
            NGS data implemented
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-13338407|
COMMENT     VNTDBDATE|-13338407|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pECFP-p65|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             1351..3003
                     /vntifkey="4"
                     /label=p65
     promoter        6330..568
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             613..1329
                     /vntifkey="4"
                     /label=ECFP
     terminator      3111..3207
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3111..3207)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(3261..3716)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3717..3812
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     RBS             3813..3847
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        3868..4149
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4106..4181
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             4241..5035
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      5226..5295
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      6013..6339
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1471 a      1852 c      1663 g      1359 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac 
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggactcag atctcgagcc gacgaactgt tccccctcat cttcccggca 
     1381 gagccagccc aggcctctgg cccctatgtg gagatcattg agcagcccaa gcagcggggc 
     1441 atgcgcttcc gctacaagtg cgaggggcgc tccgcgggca gcatcccagg cgagaggagc 
     1501 acagatacca ccaagaccca ccccaccatc aagatcaatg gctacacagg accagggaca 
     1561 gtgcgcatct ccctggtcac caaggaccct cctcaccggc ctcaccccca cgagcttgta 
     1621 ggaaaggact gccgggatgg cttctatgag gctgagctct gcccggaccg ctgcatccac 
     1681 agtttccaga acctgggaat ccagtgtgtg aagaagcggg acctggagca ggctatcagt 
     1741 cagcgcatcc agaccaacaa caaccccttc caagttccta tagaagagca gcgtggggac 
     1801 tacgacctga atgctgtgcg gctctgcttc caggtgacag tgcgggaccc atcaggcagg 
     1861 cccctccgcc tgccgcctgt cctttctcat cccatctttg acaatcgtgc ccccaacact 
     1921 gccgagctca agatctgccg agtgaaccga aactctggca gctgcctcgg tggggatgag 
     1981 atcttcctac tgtgtgacaa ggtgcagaaa gaggacattg aggtgtattt cacgggacca 
     2041 ggctgggagg cccgaggctc cttttcgcaa gctgatgtgc accgacaagt ggccattgtg 
     2101 ttccggaccc ctccctacgc agaccccagc ctgcaggctc ctgtgcgtgt ctccatgcag 
     2161 ctgcggcggc cttccgaccg ggagctcagt gagcccatgg aattccagta cctgccagat 
     2221 acagacgatc gtcaccggat tgaggagaaa cgtaaaagga catatgagac cttcaagagc 
     2281 atcatgaaga agagtccttt cagcggaccc accgaccccc ggcctccacc tcgacgcatt 
     2341 gctgtgcctt cccgcagctc agcttctgtc cccaagccag caccccagcc ctatcccttt 
     2401 acgtcatccc tgagcaccat caactatgat gagtttccca ccatggtgtt tccttctggg 
     2461 cagatcagcc aggcctcggc cttggccccg gcccctcccc aagtcctgcc ccaggctcca 
     2521 gcccctgccc ctgctccagc catggtatca gctctggccc aggccccagc ccctgtccca 
     2581 gtcctagccc caggccctcc tcaggctgtg gccccacctg cccccaagcc cacccaggct 
     2641 ggggaaggaa cgctgtcaga ggccctgctg cagctgcagt ttgatgatga agacctgggg 
     2701 gccttgcttg gcaacagcac agacccagct gtgttcacag acctggcatc cgtcgacaac 
     2761 tccgagtttc agcagctgct gaaccagggc atacctgtgg ccccccacac aactgagccc 
     2821 atgctgatgg agtaccctga ggctataact cgcctagtga caggggccca gaggcccccc 
     2881 gacccagctc ctgctccact gggggccccg gggctcccca atggcctcct ttcaggagat 
     2941 gaagacttct cctccattgc ggacatggac ttctcagccc tgctgagtca gatcagctcc 
     3001 taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt 
     3061 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 
     3121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     3181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     3241 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 
     3301 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     3361 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     3421 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     3481 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     3541 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3661 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 
     3721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     3781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     3841 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 
     3901 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     3961 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     4021 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     4081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     4141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     4201 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     4261 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     4321 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     4381 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 
     4441 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     4501 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     4561 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     4621 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     4681 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     4741 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 
     4801 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     4861 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     4921 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     4981 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     5041 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     5101 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     5161 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 
     5221 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 
     5281 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 
     5341 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 
     5401 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 
     5461 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 
     5521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     5581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     5641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     5701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     5761 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 
     5821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     5881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     5941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     6001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     6061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     6121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     6181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     6241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     6301 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 
//
