LOCUS       pET-28a-MBP-TEV-Derp10      7293 bp    DNA     circular SYN 12-AUG-2021
DEFINITION  LMBP 11981.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11981
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-AUG-2021
            NGS data implemented
FEATURES             Location/Qualifiers
     promoter        209..250
                     /label=T7\promoter\with\lac\operator
     RBS             251..294
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     misc_feature    308..325
                     /label=His-tag
     misc_feature    335..352
                     /label=thrombin\recognition\site
     CDS             368..1477
                     /label=malE
     misc_feature    1481..1501
                     /label=TEV\recognition\site
     CDS             1505..2359
                     /label=Der\p\10
     misc_feature    2366..2383
                     /label=His-tag
     terminator      2450..2497
                     /label=T7\terminator
     rep_origin      2534..2989
                     /label=f1\ori
     CDS             complement(3081..3896)
                     /label=Tn903\neomycin\resistance
     rep_origin      4388..4790
                     /label=pMB1\ori
     CDS             complement(5033..5224)
                     /label=rop
     CDS             complement(6033..7115)
                     /label=lacI
     promoter        complement(7116..7193)
                     /label=lacI\promoter,\incl.\RBS
ORIGIN
        1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
       61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
      121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA
      181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG
      241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG
      301 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC
      361 TAGCGGTAAA ATCGAAGAAG GTAAACTGGT AATCTGGATT AACGGCGATA AAGGCTATAA
      421 CGGTCTCGCT GAAGTCGGTA AGAAATTCGA GAAAGATACC GGAATTAAAG TCACCGTTGA
      481 GCATCCGGAT AAACTGGAAG AGAAATTCCC ACAGGTTGCG GCAACTGGCG ATGGCCCTGA
      541 CATTATCTTC TGGGCACACG ACCGCTTTGG TGGCTACGCT CAATCTGGCC TGTTGGCTGA
      601 AATCACCCCG GACAAAGCGT TCCAGGACAA GCTGTATCCG TTTACCTGGG ATGCCGTACG
      661 TTACAACGGC AAGCTGATTG CTTACCCGAT CGCTGTTGAA GCGTTATCGC TGATTTATAA
      721 CAAAGATCTG CTGCCGAACC CGCCAAAAAC CTGGGAAGAG ATCCCGGCGC TGGATAAAGA
      781 ACTGAAAGCG AAAGGTAAGA GCGCGCTGAT GTTCAACCTG CAAGAACCGT ACTTCACCTG
      841 GCCGCTGATT GCTGCTGACG GGGGTTATGC GTTCAAGTAT GAAAACGGCA AGTACGACAT
      901 TAAAGACGTG GGCGTGGATA ACGCTGGCGC GAAAGCGGGT CTGACCTTCC TGGTTGACCT
      961 GATTAAAAAC AAACACATGA ATGCAGACAC CGATTACTCC ATCGCAGAAG CTGCCTTTAA
     1021 TAAAGGCGAA ACAGCGATGA CCATCAACGG CCCGTGGGCA TGGTCCAACA TCGACACCAG
     1081 CAAAGTGAAT TATGGTGTAA CGGTACTGCC GACCTTCAAG GGTCAACCAT CCAAACCGTT
     1141 CGTTGGCGTG CTGAGCGCAG GTATTAACGC CGCCAGTCCG AACAAAGAGC TGGCGAAAGA
     1201 GTTCCTCGAA AACTATCTGC TGACTGATGA AGGTCTGGAA GCGGTTAATA AAGACAAACC
     1261 GCTGGGTGCC GTAGCGCTGA AGTCTTACGA GGAAGAGTTG GCGAAAGATC CACGTATTGC
     1321 CGCCACCATG GAAAACGCCC AGAAAGGTGA AATCATGCCG AACATCCCGC AGATGTCCGC
     1381 TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC AGACTGTCGA
     1441 TGAAGCCCTG AAAGACGCGC AGACTCGTAT CACCAAGGGT GAAAATCTAT ACTTCCAAGG
     1501 ATCCATGGAA GCCATCAAAA ACAAAATGCA GGCAATGAAA CTGGAAAAGG ATAATGCAAT
     1561 TGATCGTGCC GAAATTGCAG AACAGAAAGC ACGTGATGCA AATCTGCGTG CAGAAAAAAG
     1621 CGAAGAAGAA GTTCGCGCAC TGCAGAAGAA AATTCAGCAG ATTGAAAATG AACTGGACCA
     1681 GGTTCAAGAA CAGCTGAGCG CAGCAAATAC CAAACTGGAA GAAAAAGAAA AAGCCCTGCA
     1741 GACAGCCGAA GGTGATGTTG CAGCACTGAA TCGTCGTATT CAGCTGATCG AAGAAGATCT
     1801 GGAACGTAGC GAAGAACGTC TGAAAATTGC AACCGCAAAA TTAGAAGAAG CAAGCCAGAG
     1861 CGCAGATGAA AGCGAACGTA TGCGTAAAAT GCTGGAACAT CGTAGCATTA CCGATGAAGA
     1921 ACGTATGGAA GGTCTGGAAA ACCAGCTGAA AGAAGCACGT ATGATGGCAG AAGATGCAGA
     1981 TCGTAAATAT GATGAAGTTG CACGTAAACT GGCAATGGTT GAAGCCGACC TGGAACGTGC
     2041 AGAGGAACGC GCAGAAACCG GTGAAAGCAA AATTGTTGAG CTGGAAGAAG AACTGCGCGT
     2101 TGTTGGTAAT AATCTGAAAA GCCTGGAAGT GAGTGAAGAG AAAGCACAGC AGCGTGAAGA
     2161 AGCACACGAA CAGCAGATTC GTATTATGAC CACAAAACTG AAAGAGGCAG AAGCTCGCGC
     2221 AGAATTTGCA GAACGTAGTG TTCAGAAACT GCAGAAAGAA GTTGGTCGTC TGGAAGATGA
     2281 GCTGGTTCAT GAAAAAGAGA AATACAAAAG CATCAGCGAC GAACTGGATC AGACCTTTGC
     2341 CGAACTGACC GGTTATTAAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA
     2401 CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC
     2461 CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG
     2521 ATTGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG
     2581 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT
     2641 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA
     2701 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT
     2761 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG
     2821 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT
     2881 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT
     2941 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT
     3001 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     3061 ATCCGCTCAT GAATTAATTC TTAGAAAAAC TCATCGAGCA TCAAATGAAA CTGCAATTTA
     3121 TTCATATCAG GATTATCAAT ACCATATTTT TGAAAAAGCC GTTTCTGTAA TGAAGGAGAA
     3181 AACTCACCGA GGCAGTTCCA TAGGATGGCA AGATCCTGGT ATCGGTCTGC GATTCCGACT
     3241 CGTCCAACAT CAATACAACC TATTAATTTC CCCTCGTCAA AAATAAGGTT ATCAAGTGAG
     3301 AAATCACCAT GAGTGACGAC TGAATCCGGT GAGAATGGCA AAAGTTTATG CATTTCTTTC
     3361 CAGACTTGTT CAACAGGCCA GCCATTACGC TCGTCATCAA AATCACTCGC ATCAACCAAA
     3421 CCGTTATTCA TTCGTGATTG CGCCTGAGCG AGACGAAATA CGCGATCGCT GTTAAAAGGA
     3481 CAATTACAAA CAGGAATCGA ATGCAACCGG CGCAGGAACA CTGCCAGCGC ATCAACAATA
     3541 TTTTCACCTG AATCAGGATA TTCTTCTAAT ACCTGGAATG CTGTTTTCCC GGGGATCGCA
     3601 GTGGTGAGTA ACCATGCATC ATCAGGAGTA CGGATAAAAT GCTTGATGGT CGGAAGAGGC
     3661 ATAAATTCCG TCAGCCAGTT TAGTCTGACC ATCTCATCTG TAACATCATT GGCAACGCTA
     3721 CCTTTGCCAT GTTTCAGAAA CAACTCTGGC GCATCGGGCT TCCCATACAA TCGATAGATT
     3781 GTCGCACCTG ATTGCCCGAC ATTATCGCGA GCCCATTTAT ACCCATATAA ATCAGCATCC
     3841 ATGTTGGAAT TTAATCGCGG CCTAGAGCAA GACGTTTCCC GTTGAATATG GCTCATAACA
     3901 CCCCTTGTAT TACTGTTTAT GTAAGCAGAC AGTTTTATTG TTCATGACCA AAATCCCTTA
     3961 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     4021 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
     4081 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
     4141 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
     4201 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
     4261 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
     4321 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
     4381 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
     4441 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
     4501 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
     4561 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
     4621 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT
     4681 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
     4741 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG
     4801 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC AATGGTGCAC TCTCAGTACA
     4861 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG
     4921 TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC
     4981 TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT
     5041 TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT
     5101 GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA
     5161 GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG
     5221 TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC
     5281 GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT
     5341 GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC
     5401 AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG
     5461 CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG
     5521 AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC
     5581 AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC
     5641 GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGGGCCGCCA
     5701 TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG
     5761 CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC
     5821 TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA
     5881 GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC
     5941 GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGAGATCCC GGTGCCTAAT
     6001 GAGTGAGCTA ACTTACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
     6061 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
     6121 GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC
     6181 GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA
     6241 TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT
     6301 CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG
     6361 CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC
     6421 ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC
     6481 GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG
     6541 ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC
     6601 TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG
     6661 TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA
     6721 TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG
     6781 TGCACCGCCG CTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG
     6841 GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG
     6901 GCCAGACTGG AGGTGGCAAC GCCAATCAGC AACGACTGTT TGCCCGCCAG TTGTTGTGCC
     6961 ACGCGGTTGG GAATGTAATT CAGCTCCGCC ATCGCCGCTT CCACTTTTTC CCGCGTTTTC
     7021 GCAGAAACGT GGCTGGCCTG GTTCACCACG CGGGAAACGG TCTGATAAGA GACACCGGCA
     7081 TACTCTGCGA CATCGTATAA CGTTACTGGT TTCACATTCA CCACCCTGAA TTGACTCTCT
     7141 TCCGGGCGCT ATCATGCCAT ACCGCGAAAG GTTTTGCGCC ATTCGATGGT GTCCGGGATC
     7201 TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC
     7261 GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATG
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