LOCUS       pYES2-TruMDR2      9808 bp    DNA     circular UNA 
DEFINITION  LMBP 12058
FEATURES             Location/Qualifiers
     CDS             complement(923..1783)
                     /label="ampicillin resistance"
     CDS             complement(1879..2682)
                     /label="URA3"
     rep_origin      complement(3499..4376)
                     /label="2mu ori"
     rep_origin      complement(4445..4900)
                     /label="f1 ori"
     promoter        4922..5313
                     /label="GAL1 promoter"
     promoter        5395..5411
                     /label="T7 promoter"
     CDS             5448..9443
                     /label="TruMDR2"
     terminator      9452..9727
                     /label="CYC1 terminator"
ORIGIN
        1 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
       61 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
      121 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
      181 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
      241 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
      301 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
      361 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
      421 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
      481 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
      541 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
      601 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
      661 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
      721 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
      781 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
      841 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
      901 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
      961 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
     1021 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
     1081 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
     1141 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
     1201 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
     1261 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
     1321 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
     1381 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
     1441 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
     1501 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
     1561 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
     1621 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
     1681 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
     1741 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
     1801 GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT GAGTTTAGTA TACATGCATT
     1861 TACTTATAAT ACAGTTTTTT AGTTTTGCTG GCCGCATCTT CTCAAATATG CTTCCCAGCC
     1921 TGCTTTTCTG TAACGTTCAC CCTCTACCTT AGCATCCCTT CCCTTTGCAA ATAGTCCTCT
     1981 TCCAACAATA ATAATGTCAG ATCCTGTAGA GACCACATCA TCCACGGTTC TATACTGTTG
     2041 ACCCAATGCG TCTCCCTTGT CATCTAAACC CACACCGGGT GTCATAATCA ACCAATCGTA
     2101 ACCTTCATCT CTTCCACCCA TGTCTCTTTG AGCAATAAAG CCGATAACAA AATCTTTGTC
     2161 GCTCTTCGCA ATGTCAACAG TACCCTTAGT ATATTCTCCA GTAGATAGGG AGCCCTTGCA
     2221 TGACAATTCT GCTAACATCA AAAGGCCTCT AGGTTCCTTT GTTACTTCTT CTGCCGCCTG
     2281 CTTCAAACCG CTAACAATAC CTGGGCCCAC CACACCGTGT GCATTCGTAA TGTCTGCCCA
     2341 TTCTGCTATT CTGTATACAC CCGCAGAGTA CTGCAATTTG ACTGTATTAC CAATGTCAGC
     2401 AAATTTTCTG TCTTCGAAGA GTAAAAAATT GTACTTGGCG GATAATGCCT TTAGCGGCTT
     2461 AACTGTGCCC TCCATGGAAA AATCAGTCAA GATATCCACA TGTGTTTTTA GTAAACAAAT
     2521 TTTGGGACCT AATGCTTCAA CTAACTCCAG TAATTCCTTG GTGGTACGAA CATCCAATGA
     2581 AGCACACAAG TTTGTTTGCT TTTCGTGCAT GATATTAAAT AGCTTGGCAG CAACAGGACT
     2641 AGGATGAGTA GCAGCACGTT CCTTATATGT AGCTTTCGAC ATGATTTATC TTCGTTTCCT
     2701 GCAGGTTTTT GTTCTGTGCA GTTGGGTTAA GAATACTGGG CAATTTCATG TTTCTTCAAC
     2761 ACTACATATG CGTATATATA CCAATCTAAG TCTGTGCTCC TTCCTTCGTT CTTCCTTCTG
     2821 TTCGGAGATT ACCGAATCAA AAAAATTTCA AGGAAACCGA AATCAAAAAA AAGAATAAAA
     2881 AAAAAATGAT GAATTGAAAA GCTAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTAA
     2941 TTCCACGGAC TATAGACTAT ACCTAGTATA CTCCGTCTAC TGTACGATAC ACTTCCGCTC
     3001 AGGTCCTTGT CCTTTAACGA GGCCTTACCA CTCTTTTGTT ACTCTATTGA TCCAGCTCAG
     3061 CAAAGGCAGT GTGATCTAAG ATTCTATCTT CGCGATGTAG TAAAACTAGC TAGACCGAGA
     3121 AAGAGACTAG AAATGCAAAA GGCACTTCTA CAATGGCTGC CATCATTATT ATCCGATGTG
     3181 ACGCTGCAGC TTCTCAATGA TATTCGAATA CGCTTTGAGG AGATACAGCC TAATATCCGA
     3241 CAAACTGTTT TACAGATTTA CGATCGTACT TGTTACCCAT CATTGAATTT TGAACATCCG
     3301 AACCTGGGAG TTTTCCCTGA AACAGATAGT ATATTTGAAC CTGTATAATA ATATATAGTC
     3361 TAGCGCTTTA CGGAAGACAA TGTATGTATT TCGGTTCCTG GAGAAACTAT TGCATCTATT
     3421 GCATAGGTAA TCTTGCACGT CGCATCCCCG GTTCATTTTC TGCGTTTCCA TCTTGCACTT
     3481 CAATAGCATA TCTTTGTTAA CGAAGCATCT GTGCTTCATT TTGTAGAACA AAAATGCAAC
     3541 GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA ACAGAAATGC
     3601 AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA AAACAAAAAT
     3661 GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA
     3721 AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA
     3781 AAAATGCATC CCGAGAGCGC TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC
     3841 TTTGTGCGCT CTATAATGCA GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT
     3901 AGAAGAAGGC TACTTTGGTG TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC
     3961 CGCGTTTACT GATTACTAGC GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG
     4021 ATTATATTCT ATACCGATGT GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA
     4081 TGATTCTTCA TTGGTCAGAA AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC
     4141 GTATAGGAAA TGTTTACATT TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT
     4201 ACAATTTTTT TGTCTAAAGA GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT
     4261 TTAGATGCAA GTTCAAGGAG CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG
     4321 AGATATATAG CAAAGAGATA CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATGGGA
     4381 AGCTCCACCC CGGTTGATAA TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT
     4441 ATTTAAATTG TAAGCGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC
     4501 TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC
     4561 GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC
     4621 TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA
     4681 CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG
     4741 AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG
     4801 AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC
     4861 ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT GGGGATCGAT CCACTAGTCC
     4921 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
     4981 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
     5041 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
     5101 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
     5161 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
     5221 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
     5281 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
     5341 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
     5401 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCAACatg gttgaaccga
     5461 gcgagaaacc gaatacccag aacgacgatg tcagcaagca ggaaattcga aacccagttt
     5521 cgtcgtcatc gtcaacttcc gataaggaaa aggttgctaa gaaggggaac tcggatgcca
     5581 caaagagctt gaccccggag gatctagatg ctcaacttgc tcacctgccc gagcacgaac
     5641 gagagatact caagcagcaa ttgttcatcc ctgaggtgaa ggctacctac ggcacattat
     5701 ttcgatatgc aaccaggaat gatatgatac tcctcgccat cgtttcttta gcttctattg
     5761 cagccggtgc cgccctaccg ctatttacag tcctttttgg ttccctcgcc ggcacatttc
     5821 gagatattgc tctccataga atcacctatg atgagttcaa cagtatattg acgagaaatt
     5881 ctctctactt cgtttacctt ggaattgccc aattcattct cctctatgta tccaccgtgg
     5941 gtttcatcta cgttggtgaa cacatcactc agaagatacg tgcaagatat ctccatgcaa
     6001 ttctacgcca gaacatcggt ttcttcgaca agcttggggc tggagaagtc accactcgca
     6061 tcacggcaga cacaaatctt atccaagatg gaatatcaga aaaagttggc ttgactctga
     6121 ctgccctatc gaccttcttc agcgccttta ttatcggata cgttcgatac tggaagcttg
     6181 cactgatctg cacctcgact attgtcgcga tggttctggt tatgggtggt atatctaggt
     6241 tcgtggtcaa gtctgggagg atgacattgg taagctatgg tgaaggaggc actgttgcag
     6301 aggaagtgat cagctctatc agaaatgcca ccgcctttgg aacccaagag aaacttgcaa
     6361 ggcagtacga agtgcatttg agagaagcga gaaaatgggg gcgcaggctt caaatgatgt
     6421 tgggcatcat gtttggctct atgatggcta tcatgtactc aaactacggt ctcggatttt
     6481 ggatgggatc acgcttcctc gtcggtggcg aaacagacct gtctgccatc gtcaacatcc
     6541 tccttgcgat tgttattgga tcctttagca ttggaaatgt tgctcctaat acccaggctt
     6601 tcgcttctgc tatttccgcc ggtgctaaga ttttcagcac tatcgaccgt gtttcagcaa
     6661 ttgacccagg ttctgatgag ggagatacca ttgaaaacgt ggagggaact atcgagttcc
     6721 gtggtattaa gcatatctac ccatcccgcc ctgaggttgt tgtcatggaa gatattaacc
     6781 ttgttgtccc caagggcaaa accactgctc ttgttggccc ctcaggttcc ggaaagagta
     6841 ccgttgttgg gttgttggag cgattctata acccagttgc tggctctgtt ttcctggatg
     6901 gccgtgatat caagaccctc aacctccgat ggctccgtca acaaatctcc cttgtcagcc
     6961 aagaaccaac gttattcgga actactattt ttgaaaacat ccgattaggg ttgattggct
     7021 ccccaatgga aaacgaatcc gaggagcaaa tcaaggaacg gatcgtcagt gcggccaagg
     7081 aggccaatgc tcatgacttc gttatggggc tgccggatgg ctatgcaacg gatgtcggtc
     7141 agcgtggttt ccttttatcc ggtggccaga agcagcgtat tgctattgct cgtgccattg
     7201 taagcgatcc taaaatattg ctgcttgatg aagccacgtc tgctctcgat accaagtccg
     7261 agggcgtagt acaagccgct cttgatgccg cctctagagg aagaaccact attgtcatcg
     7321 cccaccgttt atccactatt aaatctgctg ataacattgt tgtgattgtc ggtggccgca
     7381 ttgctgaaca gggtactcac gatgagctcg ttgataagaa aggcacatac cttcaactcg
     7441 ttgaagctca aaagatcaat gaggagcgtg gagaagaatc cgaggatgag gcggtgctcg
     7501 agaaggagaa ggaaatctcg cgccagattt ctgttcctgc aaagagtgtc aactccggaa
     7561 aataccctga tgaagatgtt gaggccaatc tcggacgaat agataccaag aagtccctct
     7621 ccagcgttat actgtcccaa aaacgtggtc aagagaaaga aaccgaatac tcacttggga
     7681 ctttgatacg attcatagct ggattcaaca agccagagag attgataatg ctctgtggat
     7741 tcttctttgc cgttctttct ggtgctggcc agcccgttca atccgtcttc tttgccaagg
     7801 gtatcacaac actttctctc ccgccatcat tatatggcaa attgcgcgaa gatgccaatt
     7861 tttggtccct gatgttcttg atgttgggtc tggtccagct gatcacacag tccgcccagg
     7921 gtgttatctt cgctttgtgc tctgagagtt tgatttatcg agcacgcagc aaatccttcc
     7981 gtgcaatgct tcgacaagat attgccttct tcgatctctc tgagaacagc actggtgcat
     8041 taacttcgtt cctctcaacc gaaacgaagc atctttctgg cgtgagcggc gctaccctcg
     8101 gaacaatcct gatggttagc actactctca tcgttgcttt gactgttgct ctcgcctttg
     8161 gatggaaact tgcccttgtc tgcatttcca ctgtccctgt ccttcttctc tgcggattct
     8221 atcgattttg gattctcgca caattccaaa caagagcaaa aaaggcatac gagtcttctg
     8281 caagctacgc ttgtgaagcc acttcctcta tccgcactgt tgcttctctt actagagaaa
     8341 acggcgtaat ggagatttac gaaggccaac ttaatgatca ggcaaagaag agtttgagat
     8401 ctgttgccaa atcctcgctt ctatatgctg cttcgcaatc tttctccttc ttctgtcttg
     8461 ctcttggctt ctggtatggc ggcggccttc ttggaaaggg agaatataat gcattccaat
     8521 ttttcttgtg catctcttgc gttatctttg gctctcagtc tgctggaatc gtcttttcct
     8581 tctcacctga tatgggcaag gcaaagagtg cagccgctga tttcaagagg ttatttgacc
     8641 gtgtgccaac aattgacatt gagtcacccg acggcgagaa gcttgaaacc gttgaaggta
     8701 ctattgagtt ccgcgatgtg catttcaggt accccacacg tccagaacag cctgtgcttc
     8761 gtggcctcaa cctcactgtc aagcctggcc aatatattgc attggttggg cctagtggat
     8821 gtggcaagag tactaccatt gctcttgtag aaaggttcta cgacacatta tctggcggtg
     8881 tatatattga cggaaaggac atttcaaggc ttaacgttaa ctcttaccga agccatctag
     8941 cgctcgttag tcaggagcct actctctacc agggaacaat acgggacaac gttctcctcg
     9001 gagtcgacag agatgacgtc cctgatgagc aagtgtttgc tgcatgcaag gctgccaaca
     9061 tatatgactt catcatgtcc ctgcccgatg gttttgccac tgttgtcggg agcaagggaa
     9121 gtatgctatc tggtggacag aagcaacgta ttgcgatcgc ccgtgcttta atacgagatc
     9181 caaaggttct cctccttgac gaggctacat cagcacttga ctctgaatcc gaaaaggtgg
     9241 ttcaagctgc ccttgatgcc gctgcgaagg gtcgaacgac tattgctgtt gctcaccgac
     9301 tcagtactat tcaaaaggca gacattatct atgtcttcga tcagggccgc attgttgaga
     9361 gtggcactca tcacgaactc cttcaaaata agggccgata ttatgagttg gttcatatgc
     9421 agagccttga gaaaactcat taaGCGGCCG CTCGAGCATG CATCTAGAGG GCCGCATCAT
     9481 GTAATTAGTT ATGTCACGCT TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA
     9541 GGAAGGAGTT AGACAACCTG AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT
     9601 ATTAAGAACG TTATTTATAT TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC
     9661 ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT
     9721 TTGCAAGCTG CGGCCCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     9781 ATTGGGCGCT CTTCCGCTTC CTCGCTCA
//
