LOCUS       pLPV3Z      5526 bp    DNA     circular SYN 7-FEB-2022
DEFINITION  LMBP 12691.
COMMENT     Accession number: LMBP 12691
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 7-FEB-2022
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      564..966
                     /label=pMB1\ori
     promoter        974..990
                     /label=T3\promoter
     RBS             1110..1121
                     /label=synthetic\RBS
     CDS             1128..1844
                     /label=GFP\mutant
     terminator      1864..1958
                     /label=Rho-independent\terminator
     promoter        2014..2109
                     /label=ampicillin\resistance\promoter
     CDS             2148..2522
                     /label=zeocin\resistance
     promoter        complement(2627..2643)
                     /label=T7\promoter
     promoter        2882..2977
                     /label=ampicillin\resistance\promoter
     RBS             2978..3012
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        3030..3058
                     /label=PcS\promoter
     CDS             3247..3780
                     /label=gentamicin\resistance
     CDS             4001..4294
                     /label=Acinetobacter\paaA2-like
     CDS             4284..4553
                     /label=Acinetobacter\parE2-like
     rep_origin      4317..5525
                     /label=pWH1277\ori
ORIGIN
        1 TGCATGTCTA CGGCATCCTC CAGGGGGGAT CACAAGTTTA CTCATATATA CTTTAGATTG
       61 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA
      121 TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
      181 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
      241 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
      301 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
      361 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
      421 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
      481 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
      541 TGGAGCGAAC GACCTACACC GAACAGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
      601 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
      661 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
      721 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
      781 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
      841 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
      901 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
      961 AAGAGCGCCC AGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGGTACC GGGCCCCCCC
     1021 TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCCT GCAGCCCGGG GGATCCACTA
     1081 GTTCTAGAGC GGCCGCTAAG TAAGTAATTA AAGAGGAGAA ATTAAGCATG CGTAAAGGAG
     1141 AAGAACTTTT CACTGGAGTT GTCCCAATTC TTGTTGAATT AGATGGTGAT GTTAATGGGC
     1201 ACAAATTTTC TGTCAGTGGA GAGGGTGAAG GTGATGCAAC ATACGGAAAA CTTACCCTTA
     1261 AATTTATTTG CACTACTGGA AAACTACCTG TTCCATGGCC AACACTTGTC ACTACTTTCG
     1321 GTTATGGTGT TCAATGCTTT GCGAGATACC CAGATCATAT GAAACAGCAT GACTTTTTCA
     1381 AGAGTGCCAT GCCCGAAGGT TATGTACAGG AAAGAACTAT ATTTTTCAAA GATGACGGGA
     1441 ACTACAAGAC ACGTGCTGAA GTCAAGTTTG AAGGTGATAC CCTTGTTAAT AGAATCGAGT
     1501 TAAAAGGTAT TGATTTTAAA GAAGATGGAA ACATTCTTGG ACACAAATTG GAATACAACT
     1561 ATAACTCACA CAATGTATAC ATCATGGCAG ACAAACAAAA GAATGGAATC AAAGTTAACT
     1621 TCAAAATTAG ACACAACATT GAAGATGGAA GCGTTCAACT AGCAGACCAT TATCAACAAA
     1681 ATACTCCAAT TGGCGATGGC CCTGTCCTTT TACCAGACAA CCATTACCTG TCCACACAAT
     1741 CTGCCCTTTC GAAAGATCCC AACGAAAAGA GAGACCACAT GGTCCTTCTT GAGTTTGTAA
     1801 CAGCTGCTGG GATTACACAT GGCATGGATG AACTATACAA ATAAGCTTAA TTAGCTGAGC
     1861 TTGGACTCCT GTTGATAGAT CCAGTAATGA CCTCAGAACT CCATCTGGAT TTGTTCAGAA
     1921 CGCTCGGTTG CCGCCGGGCG TTTTTTATTG GTGAGAATCC AAGCTAGCTT GAGCTCTTCT
     1981 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATCAGGTGG CACTTTTCGG GGAAATGTGC
     2041 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
     2101 AATAACCCTG ATAAATGCTT CAATAATAGC ACGTGAGGAG GGCCACCATG GCCAAGTTGA
     2161 CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG TTCTGGACCG
     2221 ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG GTCCGGGACG
     2281 ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC ACCCTGGCCT
     2341 GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC GTGTCCACGA
     2401 ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG TGGGGGCGGG
     2461 AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG GAGCAGGACT
     2521 GACACACCAA TGCATACCAA GTATGCAGCC CCTGCTGCTG TTGCTGCTGT TCAAGTGTCA
     2581 ATTGCTCTAC TCTGTTGCTC GTCTCAATGA GAGCTCCAAT TCGCCCTATA GTGAGTCGTA
     2641 TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC
     2701 CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC
     2761 CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGA AATTGTAACC
     2821 ATGGTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG
     2881 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA
     2941 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
     3001 AAAAGGAAGA GTATGAGTAT TCAACCGAAT TGACATAAGC CTGTTCGGTT CGTAAACTGT
     3061 AATGCAAGTA GCGTATGCGC TCACGCAACT GGTCCAGAAC CTTGACCGAA CGCAGCGGTG
     3121 GTAACGGCGC AGTGGCGGTT TTCATGGCTT GTTATGACTG TTTTTTTGTA CAGTCTATGC
     3181 CTCGGGCATC CAAGCAGCAA GCGCGTTACG CCGTGGGTCG ATGTTTGATG TTATGGAGCA
     3241 GCAACGATGT TACGCAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC TAAAACAAAG
     3301 TTAGGTGGCT CAAGTATGGG CATCATTCGC ACATGTAGGC TCGGCCCTGA CCAAGTCAAA
     3361 TCCATGCGGG CTGCTCTTGA TCTTTTCGGT CGTGAGTTCG GAGACGTAGC CACCTACTCC
     3421 CAACATCAGC CGGACTCCGA TTACCTCGGG AACTTGCTCC GTAGTAAGAC ATTCATCGCG
     3481 CTTGCTGCCT TCGACCAAGA AGCGGTTGTT GGCGCTCTCG CGGCTTACGT TCTGCCCAAG
     3541 TTTGAGCAGC CGCGTAGTGA GATCTATATC TATGATCTCG CAGTCTCCGG CGAGCACCGG
     3601 AGGCAGGGCA TTGCCACCGC GCTCATCAAT CTCCTCAAGC ATGAGGCCAA CGCGCTTGGT
     3661 GCTTATGTGA TCTACGTGCA AGCAGATTAC GGTGACGATC CCGCAGTGGC TCTCTATACA
     3721 AAGTTGGGCA TACGGGAAGA AGTGATGCAC TTTGATATCG ACCCAAGTAC CGCCACCTAA
     3781 CAATTCGTTC AAGCCGAGAT CGGCTTCCCG GCCGCGGAGT TGTTCGGTAA ATTGTCACAA
     3841 CGCCGCGGCC AATTCGCATC GATTGTCAGA CCAAGTATGC AGCCCCTGCT GCTGTTGCTG
     3901 CTGATGCATA CCAAGAACAG CCTAAGCCGA AAAAAGACAA TGACCTAACT TTTTAATGTG
     3961 TTACAATAGC ATTCAAATGT AACACATTGG AGTGAAAAAC ATGACAGAAG CGACTTTTAC
     4021 CTTTCGGGTA GACCATGACC TAAAGCAAGA ATTTTCAAGC CTTGCTAAGA CTGTTGATCG
     4081 GTCGGGGGCG CAGCTAATCC GTGACTTCAT GCGTGACTTT GTTAAGAAGC AACAGGAAGC
     4141 CGCAGACTAT GACAAATGGT TCAAGCAGCA GGTACAGATC GGCTTAAATG AAGCCAATGC
     4201 AGGCAAGTTG ATTCCACATG AGGACGTAAA GGCAGAGTTT GCCGCTAGAC GTGCTGCAAC
     4261 ATTGGCGAAA CTGGCAGCGA AGAATGAAGA TTGAGTGGAC TGAAACAGCT CGCCAAGATC
     4321 GTAGAAATAT CTATGATTAT CTTGAAGAAC GCAACCCTAT AGCAGCTATT GAAATTGATG
     4381 ATTTAATTGA AGAAAAGACA GATTTACTTG TTGATAATCG ACTGATGGGG CGCACAGGCA
     4441 GACAGAAAGA TACTAGGGAG TTAGTGATAC ATCCGCATTA TGTGGTTGTA TATGACATCA
     4501 CTGATATAAT ACGGATACTC AGAGTGCTAC ACACATCGCA GGAGTGGTCA TGACTTACTC
     4561 ATGTACTTTG GATTATTTAG TGTTATAAAA TCCTGATTTA TAAATTTTTT TTGTTAAAAA
     4621 AGATAAAAGC CCCTTGCAAT TGCTTGGGGC TTTACCGTAA TTTATGGGGT ACAGATCTTC
     4681 GATACTGACA TATCGGCAAT CGAAAGCATT AAGGTTTGAC GACCGCTAAT GATTTCACCA
     4741 CAGGGGCTTA ATGTACCTGT CTTAAATTCT AAGGTTTTAA CTCGCTTTGT CAAGCATAGA
     4801 CCCCAAAAAT TTAGCCAATG TCTGTAACTC AATCTGTCCA TGTGTGGGTG ATGAGGTACA
     4861 GTGACGCTAG CACACATCGG AAAAACGCTA TTACTAGGGG AACTGAACAG AGTAGCGGAC
     4921 GCAATGAGTA GTCATTTAAT TGGCGGTTAT GAGCGTGTTC AGGCGGTGCT ATCAATCGTA
     4981 ATCATAACAG TGGCAGCTTG ATACAGTGAT GTCATCCCTG ATGCGAAAGC GACCGACCGA
     5041 CGGTACATCG AATGGGAATA CTTTAGGGTG ATTTTTAAGA ATCGCTCTAG GGTGAGTATT
     5101 TCCCATTCAG CTCTGCTCCC TCCCTCTGGT ACTTTAATCA AAAGCACTAC TAAACATATG
     5161 TTTTTAAATA AAAAATATTG ATATAGAGAT AATATTAGTA AGAATAATTA AACAATTGAA
     5221 TATAGATAAA TCATTGTTAA ATAAAGATTA ATTATTAAAA TGAATGTATA CTTATATATA
     5281 AATCAATGAT TTAAAATATT TGATAAAGAA AACTTTTCAA AAAAAATATA ATTGAGATTG
     5341 TGTCATTTCG GTCAATTCTT AATATGTTCC ACGCAAGTTT TAGCTATGGT GCTAAACAGA
     5401 AATTTGCTGA AAAAGAACTT TTCACTGAAC TGGTTAAAAT GTAAGCAGCC TGAGAGCCGC
     5461 CAAAAATTTT AAAAACAAAC CGCCTTAATC ATCTTCAAAA AATACCTCTA AAACCTCACC
     5521 ATTTGA
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