LOCUS       pSEVA631                3001 bp    DNA     circular SYN 02-OCT-2014
DEFINITION  LMBP 12701.
SOURCE      Cloning vector pSEVA631
  ORGANISM  Cloning vector pSEVA631
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Silva-Rocha,R., Martinez-Garcia,E., Chavarria,M., Calles,B.,
            Arce-Rodriguez,A., de las Heras,A., Paez-Espino,D.,
            Durante-Rodriguez,G., Kim,J., Nikel,P.I., Platero,R. and de
            Lorenzo,V.
  TITLE     The Standard European Vector Architecture (SEVA): a coherent
            platform for analysis and deployment of complex prokaryotic
            phenotypes
  JOURNAL   Unpublished
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Cloning vector pSEVA631"
                     /mol_type="other DNA"
                     /db_xref="taxon:1246766"
     primer_bind     9..32
                     /note="R24"
     primer_bind     complement(121..144)
                     /note="F24"
     regulatory      151..253
                     /regulatory_class="terminator"
                     /note="T0"
     gene            497..1030
                     /gene="acc3"
     CDS             497..1030
                     /gene="acc3"
                     /codon_start=1
                     /transl_table=11
                     /product="gentamicin-3-acetyltransferase"
                     /protein_id="AFV59818.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAAL
                     DLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQP
                     RSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKL
                     GIREEVMHFDIDPSTAT"
     misc_feature    1106..1351
                     /note="oriT"
     gene            complement(1360..2022)
                     /gene="rep"
     CDS             complement(1360..2022)
                     /gene="rep"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="AFV59819.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     regulatory      2896..3000
                     /regulatory_class="terminator"
                     /note="T1"
ORIGIN      
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
      121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
      181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      241 ggtgagaatc caggggtccc caataattac gatttaaatt tgacataagc ctgttcggtt
      301 cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      361 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta
      421 cagcctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
      481 ttatggagca gcaacgatgt tacgcagcag caacgatgtt acgcagcagg gcagtcgccc
      541 taaaacaaag ttaggtggct caagtatggg catcattcgc acatgtaggc tcggccctga
      601 ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt cgtgagttcg gagacgtagc
      661 cacctactcc caacatcagc cggactccga ttacctcggg aacttgctcc gtagtaagac
      721 attcatcgcg cttgctgcct tcgaccaaga agcggttgtt ggcgctctcg cggcttacgt
      781 tctgcccaag tttgagcagc cgcgtagtga gatctatatc tatgatctcg cagtctccgg
      841 agagcaccgg aggcagggca ttgccaccgc gctcatcaat ctcctcaagc atgaggccaa
      901 cgcgcttggt gcttatgtga tctacgtgca agcagattac ggtgacgatc ccgcagtggc
      961 tctctataca aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac
     1021 cgccacctaa caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa
     1081 attggacaac ggtccgcgcg ttgtcctttt ccgctgcata accctgcttc ggggtcatta
     1141 tagcgatttt ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg
     1201 ttcgtgtaga ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag
     1261 gcccacccgc gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca
     1321 acgggaatcc tgctctgcga ggctggccgt aggccggccc taccggcgcg gcagcgttac
     1381 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
     1441 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
     1501 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
     1561 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
     1621 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
     1681 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
     1741 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
     1801 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
     1861 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
     1921 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
     1981 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
     2041 ggggtgccgc acggttgcgg caccatgcgc aatcagctgc aacttttcgg cagcgcgaca
     2101 acaattatgc gttgcgtaaa agtggcagtc aattacagat tttctttaac ctacgcaatg
     2161 agctattgcg gggggtgccg caatgagctg ttgcgtaccc ccctttttta agttgttgat
     2221 ttttaagtct ttcgcatttc gccctatatc tagttctttg gtgcccaaag aagggcaccc
     2281 ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc ggcaaaaagt ggcccctccg
     2341 gggcttgttg atcgactgcg cggccttcgg ccttgcccaa ggtggcgctg cccccttgga
     2401 acccccgcac tcgccgccgt gaggctcggg gggcaggcgg gcgggcttcg cccttcgact
     2461 gcccccactc gcataggctt gggtcgttcc aggcgcgtca aggccaagcc gctgcgcggt
     2521 cgctgcgcga gccttgaccc gccttccact tggtgtccaa ccggcaagcg aagcgcgcag
     2581 gccgcaggcc ggaggctttt ccccagagaa aattaaaaaa attgatgggg caaggccgca
     2641 ggccgcgcag ttggagccgg tgggtatgtg gtcgaaggct gggtagccgg tgggcaatcc
     2701 ctgtggtcaa gctcgtgggc aggcgcagcc tgtccatcag cttgtccagc agggttgtcc
     2761 acgggccgag cgaagcgagc cagccggtgg ccgctcgcgg ccatcgtcca catatccacg
     2821 ggctggcaag ggagcgcagc gaccgcgcag ggcgaagccc ggagagcaag cccgtagggg
     2881 gggcgcgccc agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca
     2941 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
     3001 t
//