LOCUS       pG20_FLAG_Blp                5071 bp    DNA     circular SYN 03-NOV-2020
DEFINITION  LMBP 12722.
ACCESSION   MT896415
VERSION     MT896415.1
KEYWORDS    .
SOURCE      Cloning vector pG20_FLAG_Blp
  ORGANISM  Cloning vector pG20_FLAG_Blp
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
  REMARK    Publication Status: Online-Only
            DOI: 10.17912/micropub.biology.000317
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Pratt,A.I. and Kunz,H.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Cloning vector pG20_FLAG_Blp"
                     /mol_type="other DNA"
                     /db_xref="taxon:2782033"
     regulatory      30..375
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     CDS             395..946
                     /note="confers phosphinothricin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphinothricin acetyltransferase"
                     /protein_id="QOU11669.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
                     EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
                     GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
                     VGFWQLDFSLPVPPRPVLPVTEI"
     regulatory      1178..1354
                     /regulatory_class="other"
                     /note="CaMV poly(A)"
     repeat_region   1362..1384
                     /note="TDNA LB"
     rep_origin      1511..1946
                     /note="pSa"
     CDS             2237..3052
                     /note="confers kanamycin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="QOU11670.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3223..3811
                     /note="ColE1"
     repeat_region   3559..3583
                     /note="TDNA RB"
     repeat_region   3902..3926
                     /note="TDNA RB"
     regulatory      complement(4091..4340)
                     /regulatory_class="terminator"
                     /note="HSP18.2"
     misc_feature    complement(4350..4373)
                     /note="FLAG tag"
     misc_feature    complement(4374..4451)
                     /note="MCS"
     regulatory      complement(4458..5071)
                     /regulatory_class="promoter"
                     /note="pUBQ10"
ORIGIN      
        1 cgtttattac tgactcgtcg aagctttatt gagacttttc aacaaagggt aatttcggga
       61 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
      121 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
      181 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      241 gtcgttccaa ccacgacttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
      301 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      361 catttggaga ggacagccca agctgatccc ccctatgagc ccagaacgac gcccggccga
      421 catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat
      481 cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga
      541 cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc
      601 cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc
      661 caccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca
      721 cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc
      781 caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg
      841 ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag
      901 cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgaagta gatgccgacc
      961 gaacaagagc tgatttcgag aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa
     1021 atgcgagaga acggccttac gcttggtggc acagttctcg tccacagttc gctaagctcg
     1081 ctcggctggg tcgcgggagg gccggtcgca gtgattcagg aattaattcg gtacgctgaa
     1141 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
     1201 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     1261 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     1321 cctaaaacca aaatccagta ctaaaatcca gatcgataac gttacaccac aatatatcct
     1381 gccaagatct aattccgggg atcggaaatc cagaagcccg agaggttgcc gcctttcggg
     1441 ctttttcttt ttcaaaaaaa aaaatttata aaacgatctg ttgcggccgg ccgccgggtt
     1501 gtgggcaaag gcgctcgacg gtgggcaacc gcttgcggtt gtccacgggc ggagccggtg
     1561 cgcgtagcgc attgtccaca agccaagggc gaccaataat tgatatatat attcataatt
     1621 gaaaagctaa ttgaacatac tacttgctgt aactacttgc cggagcgagg ggtgtttgca
     1681 agctgttgat ctgaaagggc tattagcgtt ctcacgtgcc tttttgatta gcgatttcac
     1741 gtgaccttat tagcgatttc acgtactccg attagcgatt tcacgtaccc tgattagcga
     1801 tttcacgtgg atagtttttg gagcgggccg gaaagccccg tgaatcaagg ctttgcgggg
     1861 cattagcggt ttcacgtgga taactaccct ctatccacag gcttccgggg ataaaaaagc
     1921 ccgctcgacg gcgggctgtt ggatggggat cgcctgaatc gccccatcat ccagccagaa
     1981 agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg tgattttgaa
     2041 cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa
     2101 ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta
     2161 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2221 taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcaa ggccgcgatt
     2281 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2341 atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2401 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2461 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2521 gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga
     2581 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2641 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2701 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2761 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2821 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2881 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2941 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     3001 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcactaga
     3061 ccaatgttac acatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3301 ggatcaagag ctaccaactc ttcttccgaa ggtaactggc ttcagcagag cgcagatacc
     3361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3781 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3841 cctggccttt tgctggcctt ttgctcacat gagatctcaa acaaacacat acagcgactt
     3901 agtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga
     3961 caatctgatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     4021 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
     4081 atctgaattc cttatcttta atcatattcc atagtccata ccatagcaca tacagtagtt
     4141 atatgctgca gaagagatcc aacaaaacat tcacaatgga ttatagaaac atttgtttat
     4201 tcattataat gagatcttac attcatttaa tattagaaaa agccacaaat tcataacaca
     4261 acaagccaag aaaaaaacac aaacttaagc acacaagcta tttatttgac acaccaaata
     4321 tttcatcttc atcttcatat gagctcttac ttgtcatcgt catccttgta gtccccggga
     4381 gcggtaccct cgaggtcgac agtactatcg atggatccac tagtggcgcg ccaggcctga
     4441 gcccggttaa ctctagtctg ttaatcagaa aaactcagat taatcgacaa attcgatcgc
     4501 acaaactaga aactaacacc agatctagat agaaatcaca aatcgaagag taattattcg
     4561 acaaaactca aattatttga acaaatcgga tgatatctat gaaaccctaa tcgagaatta
     4621 agatgatatc taacgatcaa acccagaaaa tcgacttcga tctaagatta acagaatcta
     4681 aaccaaagaa catatacgaa attgggatcg aacgaaaaca aaatcgaaga ttttgagaga
     4741 ataaggaaca cagaaattta ccttgatcac ggtagagaga attgagagaa agtttttaag
     4801 attttgagaa attgaaatct gaattgtgaa gaagaagagg tctttgggta ttgttttata
     4861 gaagaagaag aagaaaagac gaggacgact aggtcacgag aaagctaagg cggtgaagca
     4921 atagctaata ataaaatgac acgtgtattg agcgttgttt acacgcaaag ttgtttttgg
     4981 ctaattgcct tatttttagg ttgaggaaaa gtatttgtgc tttgagttga taaacacgac
     5041 tcgtgtgtgc cggctgcaac cactttgacg c
//