LOCUS       pG20_Myc_Hyg                5551 bp    DNA     circular SYN 03-NOV-2020
DEFINITION  LMBP 12723.
ACCESSION   MT896416
VERSION     MT896416.1
KEYWORDS    .
SOURCE      Cloning vector pG20_Myc_Hyg
  ORGANISM  Cloning vector pG20_Myc_Hyg
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Pratt,A.I., Knoblauch,J. and Kunz,H.-H.
  TITLE     An updated pGREEN-based vector suite for cost-effective cloning in
            plant molecular biology
  JOURNAL   MicroPubl Biol (2020)
  REMARK    Publication Status: Online-Only
            DOI: 10.17912/micropub.biology.000317
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Pratt,A.I. and Kunz,H.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2020) School of Biological Sciences, Washington
            State University, 1150 NE College Ave #385, Pullman, WA 99163, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Cloning vector pG20_Myc_Hyg"
                     /mol_type="other DNA"
                     /db_xref="taxon:2782148"
     regulatory      30..375
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     CDS             395..1420
                     /note="confers resistance to hygromycin"
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin B phosphotransferase"
                     /protein_id="QOU11671.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     regulatory      1652..1828
                     /regulatory_class="other"
                     /note="CaMV poly(A)"
     repeat_region   1836..1858
                     /note="TDNA LB"
     rep_origin      1985..2420
                     /note="pSa"
     CDS             2711..3526
                     /note="confers resistance to kanamycin"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="QOU11672.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     repeat_region   3559..3583
                     /note="TDNA RB"
     rep_origin      3697..4285
                     /note="ColE1"
     repeat_region   4376..4400
                     /note="TDNA RB"
     regulatory      complement(4565..4814)
                     /regulatory_class="terminator"
                     /note="HSP18.2"
     misc_feature    complement(4824..4853)
                     /note="Myc tag"
     misc_feature    complement(4854..4931)
                     /note="MCS"
     regulatory      complement(4938..5551)
                     /regulatory_class="promoter"
                     /note="pUBQ10"
ORIGIN      
        1 cgtttattac tgactcgtcg aagctttatt gagacttttc aacaaagggt aatttcggga
       61 aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag
      121 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat tcaagatgcc
      181 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      241 gtcgttccaa ccacgacttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
      301 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      361 catttggaga ggacagccca agctgatccc ccctatgaaa aagcctgaac tcaccgcgac
      421 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      481 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      541 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
      601 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
      661 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
      721 cgctgttctt cagccggtcg cggaggctat ggatgctatc gctgcggccg atcttagcca
      781 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
      841 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
      901 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
      961 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     1021 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     1081 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     1141 cgagcggagg catccggagc ttgcaggatc gccaaggctc cgggcgtata tgctccgcat
     1201 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     1261 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     1321 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     1381 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa
     1441 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga
     1501 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc
     1561 tgggtcgcgg gagggccggt cgcagtgatt caggaattaa ttcggtacgc tgaaatcacc
     1621 agtctctctc tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt
     1681 cccgataagg gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     1741 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     1801 accaaaatcc agtactaaaa tccagatcga taacgttaca ccacaatata tcctgccaag
     1861 atctaattcc ggggatcgga aatccagaag cccgagaggt tgccgccttt cgggcttttt
     1921 ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg ccggccgccg ggttgtgggc
     1981 aaaggcgctc gacggtgggc aaccgcttgc ggttgtccac gggcggagcc ggtgcgcgta
     2041 gcgcattgtc cacaagccaa gggcgaccaa taattgatat atatattcat aattgaaaag
     2101 ctaattgaac atactacttg ctgtaactac ttgccggagc gaggggtgtt tgcaagctgt
     2161 tgatctgaaa gggctattag cgttctcacg tgcctttttg attagcgatt tcacgtgacc
     2221 ttattagcga tttcacgtac tccgattagc gatttcacgt accctgatta gcgatttcac
     2281 gtggatagtt tttggagcgg gccggaaagc cccgtgaatc aaggctttgc ggggcattag
     2341 cggtttcacg tggataacta ccctctatcc acaggcttcc ggggataaaa aagcccgctc
     2401 gacggcgggc tgttggatgg ggatcgcctg aatcgcccca tcatccagcc agaaagtgag
     2461 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg
     2521 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc
     2581 aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat gttacattgc
     2641 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
     2701 aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcaaggccgc gattaaattc
     2761 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2821 tgcgacaatc taccgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2881 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2941 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3001 caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3061 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3121 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3181 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3241 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3301 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3361 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3421 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3481 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcac tagaccaatg
     3541 ttacacatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3781 agagctacca actcttcttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4321 cttttgctgg ccttttgctc acatgagatc tcaaacaaac acatacagcg acttagttta
     4381 cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct
     4441 gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag
     4501 ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca gccgatctga
     4561 attccttatc tttaatcata ttccatagtc cataccatag cacatacagt agttatatgc
     4621 tgcagaagag atccaacaaa acattcacaa tggattatag aaacatttgt ttattcatta
     4681 taatgagatc ttacattcat ttaatattag aaaaagccac aaattcataa cacaacaagc
     4741 caagaaaaaa acacaaactt aagcacacaa gctatttatt tgacacacca aatatttcat
     4801 cttcatcttc atatgagctc ttacaagtct tcctccgaga tcagcttctg ctccccggga
     4861 gcggtaccct cgaggtcgac agtactatcg atggatccac tagtggcgcg ccaggcctga
     4921 gcccggttaa ctctagtctg ttaatcagaa aaactcagat taatcgacaa attcgatcgc
     4981 acaaactaga aactaacacc agatctagat agaaatcaca aatcgaagag taattattcg
     5041 acaaaactca aattatttga acaaatcgga tgatatctat gaaaccctaa tcgagaatta
     5101 agatgatatc taacgatcaa acccagaaaa tcgacttcga tctaagatta acagaatcta
     5161 aaccaaagaa catatacgaa attgggatcg aacgaaaaca aaatcgaaga ttttgagaga
     5221 ataaggaaca cagaaattta ccttgatcac ggtagagaga attgagagaa agtttttaag
     5281 attttgagaa attgaaatct gaattgtgaa gaagaagagg tctttgggta ttgttttata
     5341 gaagaagaag aagaaaagac gaggacgact aggtcacgag aaagctaagg cggtgaagca
     5401 atagctaata ataaaatgac acgtgtattg agcgttgttt acacgcaaag ttgtttttgg
     5461 ctaattgcct tatttttagg ttgaggaaaa gtatttgtgc tttgagttga taaacacgac
     5521 tcgtgtgtgc cggctgcaac cactttgacg c
//