LOCUS       pPTK066_P6_Lox71-BlastR-Lox66      3007 bp    DNA     circular UNA 30-NOV-2023
DEFINITION  LMBP 12777.
COMMENT     Accession number: LMBP 12777
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 30-NOV-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     CDS             complement(112..771)
                     /label=chloramphenicol\resistance
     rep_origin      1323..1641
                     /label=pMB1\ori
     misc_recomb     1681..1714
                     /label=Lox71
     promoter        1715..2271
                     /label=ILV5\promoter
     promoter        2272..2336
                     /label=synthetic\EM72\promoter
     CDS             2337..2735
                     /label=blasticidin\resistance
     terminator      2736..2937
                     /label=E. gossypii\TEF1\terminator
     misc_recomb     2938..2971
                     /label=Lox66
ORIGIN
        1 AGACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA ACAAATCCAG ATGGAGTTCT
       61 GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC TCGATATCAA ATTACGCCCC
      121 GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC
      181 ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT
      241 ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA
      301 ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA
      361 CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG
      421 TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG
      481 CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT
      541 CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC
      601 CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG
      661 AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG
      721 ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC
      781 CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT
      841 ATGGTGAAAG TTGGAACCTC TTACGTGCCC GATCAATCAT GACCAAAATC CCTTAACGTG
      901 AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC
      961 CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG
     1021 TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG
     1081 CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT
     1141 CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG
     1201 GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC
     1261 GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG
     1321 AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG
     1381 CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG
     1441 GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC
     1501 GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT
     1561 TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC
     1621 CTGATTCTGT GGATAACCGT AGTCGGTCTC ATACAACAGG GACTAAAGAG GTGATGATTA
     1681 TACCGTTCGT ATAGCATACA TTATACGAAG TTATATCCTT CAGTAATGTC TTGTTTCTTT
     1741 TGTTGCAGTG GTGAGCCATT TTGACTTCGT GAAAGTTTCT TTAGAATAGT TGTTTCCAGA
     1801 GGCCAAACAT TCCACCCGTA GTAAAGTGCA AGCGTAGGAG AACCAAGACT GGCATAAATC
     1861 AGGTATAAGT GTCGAGCACT GGCAGGTGAT CTTCTGAAAG TTTCTACTAG CAGATAAGAT
     1921 CCAGTAGTCA TGCATATGGC AACAATGTAC CGTGTGGATC TAAGAACGCG TCCTACTAAC
     1981 CTTCGCATTC GTTGGTCCAG TTTGTTGTTA TCGATCAACG TGACAAGGTT GTCGATTCCG
     2041 CGTAAGCATG CATACCCAAG GACGCCTGTT GCAATTCCAA GTGAGCCAGT TCCAACAATC
     2101 TTTGTAATAT TAGAGCACTT CATTGTGTTG CGCTTGAAAG TAAAATGCGA ACAAATTAAG
     2161 AGATAATCTC GAAACCGCGA CTTCAAACGC CAATATGATG TGCGGCACAC AATAAGCGTT
     2221 CATATCCGCT GGGTGACTTT CTCGCTTTAA AAAATTATCC GAAAAAATTT TCTAGAGTGT
     2281 TGTTACTTTA TACTTCCGGC TCGTATAATA CGACAAGGTG TAAGGAGGAC TAAACCATGG
     2341 CCAAGCCTTT GTCTCAAGAA GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA
     2401 GCATCCCCAT CTCTGAAGAT TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA
     2461 TCTTCACTGG TGTCAATGTA TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC
     2521 TGGGCACTGC TGCTGCTGCG GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG
     2581 AGAACAGGGG CATCTTGAGC CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC
     2641 CTGGGATCAA AGCCATAGTG AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG
     2701 AATTGCTGCC CTCTGGTTAT GTGTGGGAGG GCTAAAGTAA CTGACAATAA AAAGATTCTT
     2761 GTTTTCAAGA ACTTGTCATT TGTATAGTTT TTTTATATTG TAGTTGTTCT ATTTTAATCA
     2821 AATGTTAGCG TGATTTATAT TTTTTTTCGC CTCGACATCA TCTGCCCAGA TGCGAAGTTA
     2881 AGTGCGCAGA AAGTAATATC ATGCGTCAAT CGTATGTGAA TGCTGGTCGC TATACTGATA
     2941 ACTTCGTATA GCATACATTA TACGAACGGT AGACAGTATC GAATGGACTC TGATGAGAGT
     3001 TGAGACC
//
