LOCUS       pPTK197_P8_NourR      3092 bp    DNA     circular UNA 23-OCT-2023
DEFINITION  LMBP 12787.
COMMENT     Accession number: LMBP 12787
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      447..764
                     /label=pMB1\ori
     promoter        778..831
                     /label=PLtetO-1\promoter
     RBS             832..856
                     /label=synthetic\RBS
     CDS             857..1534
                     /label=mRFP1
     terminator      1555..1598
                     /label=T1\terminator
     terminator      1632..1672
                     /label=T7Te\terminator
     promoter        1688..2244
                     /label=ILV5\promoter
     promoter        2245..2309
                     /label=synthetic\EM72\promoter
     CDS             2310..2882
                     /label=nourseothricin\resistance
     terminator      2895..3092
                     /label=E.\gossypii\TEF1\terminator
ORIGIN
        1 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
       61 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
      121 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      181 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      241 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      301 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      361 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      421 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      481 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      541 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      601 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      661 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      721 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC
      781 CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA
      841 AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA
      901 AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG
      961 TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC
     1021 GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA
     1081 CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG
     1141 TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA
     1201 CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT
     1261 TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG
     1321 TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC
     1381 TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC
     1441 CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA
     1501 ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA
     1561 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
     1621 CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC
     1681 TCACCGAATC CTTCAGTAAT GTCTTGTTTC TTTTGTTGCA GTGGTGAGCC ATTTTGACTT
     1741 CGTGAAAGTT TCTTTAGAAT AGTTGTTTCC AGAGGCCAAA CATTCCACCC GTAGTAAAGT
     1801 GCAAGCGTAG GAGAACCAAG ACTGGCATAA ATCAGGTATA AGTGTCGAGC ACTGGCAGGT
     1861 GATCTTCTGA AAGTTTCTAC TAGCAGATAA GATCCAGTAG TCATGCATAT GGCAACAATG
     1921 TACCGTGTGG ATCTAAGAAC GCGTCCTACT AACCTTCGCA TTCGTTGGTC CAGTTTGTTG
     1981 TTATCGATCA ACGTGACAAG GTTGTCGATT CCGCGTAAGC ATGCATACCC AAGGACGCCT
     2041 GTTGCAATTC CAAGTGAGCC AGTTCCAACA ATCTTTGTAA TATTAGAGCA CTTCATTGTG
     2101 TTGCGCTTGA AAGTAAAATG CGAACAAATT AAGAGATAAT CTCGAAACCG CGACTTCAAA
     2161 CGCCAATATG ATGTGCGGCA CACAATAAGC GTTCATATCC GCTGGGTGAC TTTCTCGCTT
     2221 TAAAAAATTA TCCGAAAAAA TTTTCTAGAG TGTTGTTACT TTATACTTCC GGCTCGTATA
     2281 ATACGACAAG GTGTAAGGAG GACTAAACCA TGGGTACTAC CTTAGATGAT ACAGCCTACA
     2341 GATACAGAAC ATCAGTCCCT GGTGATGCTG AAGCAATTGA GGCTTTAGAC GGTTCATTCA
     2401 CCACCGACAC CGTCTTTAGA GTAACCGCCA CCGGTGATGG ATTTACCTTA AGAGAAGTCC
     2461 CAGTCGACCC TCCATTAACT AAAGTCTTTC CAGATGATGA ATCTGATGAC GAAAGCGACG
     2521 ACGGAGAAGA TGGTGACCCA GATTCAAGAA CTTTCGTAGC ATACGGTGAT GACGGTGATT
     2581 TGGCTGGTTT TGTAGTCGTT TCTTATTCAG GTTGGAATAG AAGGTTGACC GTTGAAGATA
     2641 TAGAAGTCGC CCCAGAGCAT AGAGGTCATG GTGTAGGAAG AGCTTTGATG GGTTTGGCTA
     2701 CAGAATTTGC AAGAGAGAGA GGAGCCGGTC ATTTATGGTT AGAAGTTACT AATGTTAACG
     2761 CCCCTGCTAT CCATGCTTAT AGAAGAATGG GTTTCACATT ATGTGGTTTA GATACTGCTT
     2821 TATATGATGG AACAGCATCT GACGGTGAAC AGGCCTTGTA TATGTCTATG CCTTGCCCTT
     2881 AAGCCGAGTA ACTGACTGAC AATAAAAAGA TTCTTGTTTT CAAGAACTTG TCATTTGTAT
     2941 AGTTTTTTTA TATTGTAGTT GTTCTATTTT AATCAAATGT TAGCGTGATT TATATTTTTT
     3001 TTCGCCTCGA CATCATCTGC CCAGATGCGA AGTTAAGTGC GCAGAAAGTA ATATCATGCG
     3061 TCAATCGTAT GTGAATGCTG GTCGCTATAC TG
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