LOCUS       pPTK078_P8_Lox71-KanR-Lox66      3447 bp    DNA     circular UNA 16-OCT-2023
DEFINITION  LMBP 12792.
COMMENT     Accession number: LMBP 12792
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 16-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      447..764
                     /label=pMB1\ori
     promoter        778..831
                     /label=PLtetO-1\promoter
     RBS             832..856
                     /label=synthetic\RBS
     CDS             857..1534
                     /label=mRFP1
     terminator      1555..1598
                     /label=T1\terminator
     terminator      1632..1672
                     /label=T7Te\terminator
     misc_recomb     1713..1746
                     /label=Lox71
     promoter        1747..2303
                     /label=ILV5\promoter
     promoter        2304..2368
                     /label=synthetic\EM72\promoter
     CDS             2369..3178
                     /label=Tn903\neomycin\resistance
     terminator      3183..3388
                     /label=E.\gossypii\TEF1\terminator
     misc_recomb     3389..3422
                     /label=Lox66
ORIGIN
        1 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
       61 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
      121 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
      181 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
      241 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
      301 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
      361 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
      421 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
      481 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
      541 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
      601 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
      661 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
      721 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTCCCTTG AGACCAGTCC
      781 CTATCAGTGA TAGAGATTGA CATCCCTATC AGTGATAGAG ATACTGAGCA CGGATCTGAA
      841 AGAGGAGAAA GGATCTATGG CGAGTAGCGA AGACGTTATC AAAGAGTTCA TGCGTTTCAA
      901 AGTTCGTATG GAAGGTTCCG TTAACGGTCA CGAGTTCGAA ATCGAAGGTG AAGGTGAAGG
      961 TCGTCCGTAC GAAGGTACTC AGACCGCTAA ACTGAAAGTT ACCAAAGGTG GTCCGCTGCC
     1021 GTTCGCTTGG GACATCCTGT CCCCGCAGTT CCAGTACGGT TCCAAAGCTT ACGTTAAACA
     1081 CCCGGCTGAC ATCCCGGACT ACCTGAAACT GTCCTTCCCG GAAGGTTTCA AATGGGAACG
     1141 TGTTATGAAC TTCGAAGACG GTGGTGTTGT TACCGTTACC CAGGACTCCT CCCTGCAAGA
     1201 CGGTGAGTTC ATCTACAAAG TTAAACTGCG TGGTACTAAC TTCCCGTCCG ACGGTCCGGT
     1261 TATGCAGAAA AAAACCATGG GTTGGGAAGC TTCCACCGAA CGTATGTACC CGGAAGACGG
     1321 TGCTCTGAAA GGTGAAATCA AAATGCGTCT GAAACTGAAA GACGGTGGTC ACTACGACGC
     1381 TGAAGTTAAA ACCACCTACA TGGCTAAAAA ACCGGTTCAG CTGCCGGGTG CTTACAAAAC
     1441 CGACATCAAA CTGGACATCA CCTCCCACAA CGAAGACTAC ACCATCGTTG AACAGTACGA
     1501 ACGTGCTGAA GGTCGTCACT CCACCGGTGC TTAATAAGGA TCTCCAGGCA TCAAATAAAA
     1561 CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT
     1621 CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAAGTCGGTC
     1681 TCACCGAACA GGGACTAAAG AGGTGATGAT TATACCGTTC GTATAGCATA CATTATACGA
     1741 AGTTATATCC TTCAGTAATG TCTTGTTTCT TTTGTTGCAG TGGTGAGCCA TTTTGACTTC
     1801 GTGAAAGTTT CTTTAGAATA GTTGTTTCCA GAGGCCAAAC ATTCCACCCG TAGTAAAGTG
     1861 CAAGCGTAGG AGAACCAAGA CTGGCATAAA TCAGGTATAA GTGTCGAGCA CTGGCAGGTG
     1921 ATCTTCTGAA AGTTTCTACT AGCAGATAAG ATCCAGTAGT CATGCATATG GCAACAATGT
     1981 ACCGTGTGGA TCTAAGAACG CGTCCTACTA ACCTTCGCAT TCGTTGGTCC AGTTTGTTGT
     2041 TATCGATCAA CGTGACAAGG TTGTCGATTC CGCGTAAGCA TGCATACCCA AGGACGCCTG
     2101 TTGCAATTCC AAGTGAGCCA GTTCCAACAA TCTTTGTAAT ATTAGAGCAC TTCATTGTGT
     2161 TGCGCTTGAA AGTAAAATGC GAACAAATTA AGAGATAATC TCGAAACCGC GACTTCAAAC
     2221 GCCAATATGA TGTGCGGCAC ACAATAAGCG TTCATATCCG CTGGGTGACT TTCTCGCTTT
     2281 AAAAAATTAT CCGAAAAAAT TTTCTAGAGT GTTGTTACTT TATACTTCCG GCTCGTATAA
     2341 TACGACAAGG TGTAAGGAGG ACTAAACCAT GGGTAAGGAA AAGACTCACG TTTCGAGGCC
     2401 GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT
     2461 CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG CCCGATGCGC CAGAGTTGTT
     2521 TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA
     2581 CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA
     2641 TGCATGGTTA CTCACCACTG CGATCCCCGG CAAAACAGCA TTCCAGGTAT TAGAAGAATA
     2701 TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC
     2761 GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGCCTGG CTCAGGCGCA
     2821 ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG
     2881 GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAGCTTTTG CCATTCTCAC CGGATTCAGT
     2941 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG
     3001 TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG
     3061 GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT
     3121 TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG CTCGACGAGT TCTTCTAAGC
     3181 CGAGTAACTG ACTGACAATA AAAAGATTCT TGTTTTCAAG AACTTGTCAT TTGTATAGTT
     3241 TTTTTATATT GTAGTTGTTC TATTTTAATC AAATGTTAGC GTGATTTATA TTTTTTTTCG
     3301 CCTCGACATC ATCTGCCCAG ATGCGAAGTT AAGTGCGCAG AAAGTAATAT CATGCGTCAA
     3361 TCGTATGTGA ATGCTGGTCG CTATACTGAT AACTTCGTAT AGCATACATT ATACGAACGG
     3421 TAGACAGTAT CGAATGGACT CTGATGA
//
