LOCUS       pMC_MultiGene_NourR      4790 bp    DNA     circular UNA 17-OCT-2023
DEFINITION  LMBP 12794.
COMMENT     Accession number: LMBP 12794
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-OCT-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     promoter        5..561
                     /label=ILV5\promoter
     promoter        562..626
                     /label=synthetic\EM72\promoter
     CDS             627..1199
                     /label=nourseothricin\resistance
     terminator      1204..1409
                     /label=E.\gossypii\TEF1\terminator
     CDS             complement(1652..2512)
                     /label=ampicillin\resistance
     rep_origin      3090..3407
                     /label=pMB1\ori
     misc_feature    3412..3580
                     /label=ConLS'\connector
     promoter        3585..3714
                     /label=glpT\promoter
     RBS             3715..3750
                     /label=synthetic\RBS
     CDS             3751..4467
                     /label=sfGFP
     terminator      4479..4522
                     /label=T1\terminator
     terminator      4556..4596
                     /label=T7Te\terminator
     misc_feature    4601..4790
                     /label=ConRE'\connector
ORIGIN
        1 TACAATCCTT CAGTAATGTC TTGTTTCTTT TGTTGCAGTG GTGAGCCATT TTGACTTCGT
       61 GAAAGTTTCT TTAGAATAGT TGTTTCCAGA GGCCAAACAT TCCACCCGTA GTAAAGTGCA
      121 AGCGTAGGAG AACCAAGACT GGCATAAATC AGGTATAAGT GTCGAGCACT GGCAGGTGAT
      181 CTTCTGAAAG TTTCTACTAG CAGATAAGAT CCAGTAGTCA TGCATATGGC AACAATGTAC
      241 CGTGTGGATC TAAGAACGCG TCCTACTAAC CTTCGCATTC GTTGGTCCAG TTTGTTGTTA
      301 TCGATCAACG TGACAAGGTT GTCGATTCCG CGTAAGCATG CATACCCAAG GACGCCTGTT
      361 GCAATTCCAA GTGAGCCAGT TCCAACAATC TTTGTAATAT TAGAGCACTT CATTGTGTTG
      421 CGCTTGAAAG TAAAATGCGA ACAAATTAAG AGATAATCTC GAAACCGCGA CTTCAAACGC
      481 CAATATGATG TGCGGCACAC AATAAGCGTT CATATCCGCT GGGTGACTTT CTCGCTTTAA
      541 AAAATTATCC GAAAAAATTT TCTAGAGTGT TGTTACTTTA TACTTCCGGC TCGTATAATA
      601 CGACAAGGTG TAAGGAGGAC TAAACCATGG GTACTACCTT AGATGATACA GCCTACAGAT
      661 ACAGAACATC AGTCCCTGGT GATGCTGAAG CAATTGAGGC TTTAGACGGT TCATTCACCA
      721 CCGACACCGT CTTTAGAGTA ACCGCCACCG GTGATGGATT TACCTTAAGA GAAGTCCCAG
      781 TCGACCCTCC ATTAACTAAA GTCTTTCCAG ATGATGAATC TGATGACGAA AGCGACGACG
      841 GAGAAGATGG TGACCCAGAT TCAAGAACTT TCGTAGCATA CGGTGATGAC GGTGATTTGG
      901 CTGGTTTTGT AGTCGTTTCT TATTCAGGTT GGAATAGAAG GTTGACCGTT GAAGATATAG
      961 AAGTCGCCCC AGAGCATAGA GGTCATGGTG TAGGAAGAGC TTTGATGGGT TTGGCTACAG
     1021 AATTTGCAAG AGAGAGAGGA GCCGGTCATT TATGGTTAGA AGTTACTAAT GTTAACGCCC
     1081 CTGCTATCCA TGCTTATAGA AGAATGGGTT TCACATTATG TGGTTTAGAT ACTGCTTTAT
     1141 ATGATGGAAC AGCATCTGAC GGTGAACAGG CCTTGTATAT GTCTATGCCT TGCCCTTAAG
     1201 CCGAGTAACT GACTGACAAT AAAAAGATTC TTGTTTTCAA GAACTTGTCA TTTGTATAGT
     1261 TTTTTTATAT TGTAGTTGTT CTATTTTAAT CAAATGTTAG CGTGATTTAT ATTTTTTTTC
     1321 GCCTCGACAT CATCTGCCCA GATGCGAAGT TAAGTGCGCA GAAAGTAATA TCATGCGTCA
     1381 ATCGTATGTG AATGCTGGTC GCTATACTGG AGTGTACTGA GAGTGCACCA TATGCGGTGT
     1441 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCCATTC GCCATTCAGG
     1501 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCACCGAGAT
     1561 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
     1621 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
     1681 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
     1741 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGGACCCAC
     1801 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
     1861 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
     1921 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
     1981 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
     2041 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
     2101 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
     2161 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
     2221 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
     2281 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
     2341 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
     2401 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
     2461 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
     2521 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
     2581 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
     2641 CTGTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     2701 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     2761 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     2821 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     2881 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     2941 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     3001 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     3061 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     3121 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     3181 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     3241 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     3301 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     3361 CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTCCC TGAATTCGCA
     3421 TCTAGACTGA TGAGACGTGG TAGAGCCACA AACAGCCGGT ACAAGCAACG ATCTCCAGGA
     3481 CCATCTGAAT CATGCGCGGA TGACACGAAC TCACGACGGC GATCACAGAC ATTAACCCAC
     3541 AGTACAGACA CTGCGACAAC GTGGCAATTC GTCGCAATAC AACGGAAAGT GAAACGTGAT
     3601 TTCATGCGTC ATTTTGAACA TTTTGTAAAT CTTATTTAAT AATGTGTGCG GCAATTCACA
     3661 TTTAATTTAT GAATGTTTTC TTAACATCGC GGCAACTCAA GAAACGGCAG GTTCGGATCT
     3721 TAGCTACTAG AGAAAGAGGA GAAATACTAG ATGCGTAAAG GCGAAGAGCT GTTCACTGGT
     3781 GTCGTCCCTA TTCTGGTGGA ACTGGATGGT GATGTCAACG GTCATAAGTT TTCCGTGCGT
     3841 GGCGAGGGTG AAGGTGACGC AACTAATGGT AAACTGACGC TGAAGTTCAT CTGTACTACT
     3901 GGTAAACTGC CGGTTCCTTG GCCGACTCTG GTAACGACGC TGACTTATGG TGTTCAGTGC
     3961 TTTGCTCGTT ATCCGGACCA TATGAAGCAG CATGACTTCT TCAAGTCCGC CATGCCGGAA
     4021 GGCTATGTGC AGGAACGCAC GATTTCCTTT AAGGATGACG GCACGTACAA AACGCGTGCG
     4081 GAAGTGAAAT TTGAAGGCGA TACCCTGGTA AACCGCATTG AGCTGAAAGG CATTGACTTT
     4141 AAAGAGGACG GCAATATCCT GGGCCATAAG CTGGAATACA ATTTTAACAG CCACAATGTT
     4201 TACATCACCG CCGATAAACA AAAAAATGGC ATTAAAGCGA ATTTTAAAAT TCGCCACAAC
     4261 GTGGAGGATG GCAGCGTGCA GCTGGCTGAT CACTACCAGC AAAACACTCC AATCGGTGAT
     4321 GGTCCTGTTC TGCTGCCAGA CAATCACTAT CTGAGCACGC AAAGCGTTCT GTCTAAAGAT
     4381 CCGAACGAGA AACGCGATCA TATGGTTCTG CTGGAGTTCG TAACCGCAGC GGGCATCACG
     4441 CATGGTATGG ATGAACTGTA CAAATGACCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA
     4501 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCTAC TAGAGTCACA
     4561 CTGGCTCACC TTCGGGTGGG CCTTTCTGCG TTTATAGCTG GAAATCTGCT CGTCAGTGGT
     4621 GCTCACACTG ACGAATCATG TACAGATCAT ACCGATGACT GCCTGGCGAC TCACAACTAA
     4681 GCAAGACAGC CGGAACCAGC GCCGGCGAAC ACCACTGCAT ATATGGCATA TCACAACAGT
     4741 CCACGTCTCA AGCAGTTACA GAGATGTTAC GAACCACTAG TGCACTGCAG
//
