LOCUS       pET-28a-MBP-TEV-Cypc2      7740 bp    DNA     circular SYN 29-SEP-2021
DEFINITION  LMBP 12818.
COMMENT     Accession number: LMBP 12818
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 29-SEP-2021
FEATURES             Location/Qualifiers
     promoter        209..250
                     /label=T7\promoter\with\lac\operator
     RBS             251..294
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     misc_feature    308..325
                     /label=His-tag
     misc_feature    335..352
                     /label=thrombin\recognition\site
     CDS             368..1477
                     /label=malE
     misc_feature    1481..1501
                     /label=TEV\recognition\site
     CDS             1505..2806
                     /label=Cyp\c\2
     misc_feature    2813..2830
                     /label=His-tag
     terminator      2897..2944
                     /label=T7\terminator
     rep_origin      2981..3436
                     /label=f1\ori
     CDS             complement(3528..4343)
                     /label=Tn903\neomycin\resistance
     rep_origin      4835..5237
                     /label=pMB1\ori
     CDS             complement(5480..5671)
                     /label=rop
     CDS             complement(6480..7562)
                     /label=lacI
     promoter        complement(7563..7640)
                     /label=lacI\promoter,\incl.\RBS
ORIGIN
        1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
       61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
      121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA
      181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG
      241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGG
      301 CAGCAGCCAT CATCATCATC ATCACAGCAG CGGCCTGGTG CCGCGCGGCA GCCATATGGC
      361 TAGCGGTAAA ATCGAAGAAG GTAAACTGGT AATCTGGATT AACGGCGATA AAGGCTATAA
      421 CGGTCTCGCT GAAGTCGGTA AGAAATTCGA GAAAGATACC GGAATTAAAG TCACCGTTGA
      481 GCATCCGGAT AAACTGGAAG AGAAATTCCC ACAGGTTGCG GCAACTGGCG ATGGCCCTGA
      541 CATTATCTTC TGGGCACACG ACCGCTTTGG TGGCTACGCT CAATCTGGCC TGTTGGCTGA
      601 AATCACCCCG GACAAAGCGT TCCAGGACAA GCTGTATCCG TTTACCTGGG ATGCCGTACG
      661 TTACAACGGC AAGCTGATTG CTTACCCGAT CGCTGTTGAA GCGTTATCGC TGATTTATAA
      721 CAAAGATCTG CTGCCGAACC CGCCAAAAAC CTGGGAAGAG ATCCCGGCGC TGGATAAAGA
      781 ACTGAAAGCG AAAGGTAAGA GCGCGCTGAT GTTCAACCTG CAAGAACCGT ACTTCACCTG
      841 GCCGCTGATT GCTGCTGACG GGGGTTATGC GTTCAAGTAT GAAAACGGCA AGTACGACAT
      901 TAAAGACGTG GGCGTGGATA ACGCTGGCGC GAAAGCGGGT CTGACCTTCC TGGTTGACCT
      961 GATTAAAAAC AAACACATGA ATGCAGACAC CGATTACTCC ATCGCAGAAG CTGCCTTTAA
     1021 TAAAGGCGAA ACAGCGATGA CCATCAACGG CCCGTGGGCA TGGTCCAACA TCGACACCAG
     1081 CAAAGTGAAT TATGGTGTAA CGGTACTGCC GACCTTCAAG GGTCAACCAT CCAAACCGTT
     1141 CGTTGGCGTG CTGAGCGCAG GTATTAACGC CGCCAGTCCG AACAAAGAGC TGGCGAAAGA
     1201 GTTCCTCGAA AACTATCTGC TGACTGATGA AGGTCTGGAA GCGGTTAATA AAGACAAACC
     1261 GCTGGGTGCC GTAGCGCTGA AGTCTTACGA GGAAGAGTTG GCGAAAGATC CACGTATTGC
     1321 CGCCACCATG GAAAACGCCC AGAAAGGTGA AATCATGCCG AACATCCCGC AGATGTCCGC
     1381 TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC AGACTGTCGA
     1441 TGAAGCCCTG AAAGACGCGC AGACTCGTAT CACCAAGGGT GAAAATCTAT ACTTCCAAGG
     1501 ATCTATGTCC ATCAGTAAGA TTCACGCTCG TGAGATCCTC GACTCCAGAG GAAACCCCAC
     1561 TGTGGAGGTG GATCTGTACA CGGCTAAAGG ACGTTTCCGG GCAGCTGTTC CCAGCGGCGC
     1621 TTCCACTGGA GTTCACGAGG CTCTGGAGCT CCGAGACGGA GACAAGACAC GCTACCTGGG
     1681 CAAAGGTACC CAGAAGGCTG TGGACCATGT GAACAAAGAG ATCGCTCCCA AACTGATTGA
     1741 GAAGAAGTTC AGCGTCGTGG AGCAGGAGAA GATCGACAAG TTCATGCTGG AACTCGATGG
     1801 AACGGAGAAC AAATCTAAGT TTGGTGCTAA TGCGATCCTG GGTGTGAGTC TGGCCGTGTG
     1861 TAAGGCAGGC GCGGCTGAGA AGGGCGTTCC TCTGTACCGC CACATCGCCG ACCTCGCCGG
     1921 GAACAAAGAC GTGATCCTGC CGGTTCCTGC CTTCAACGTC ATCAACGGCG GCTCTCACGC
     1981 CGGGAACAAG CTGGCCATGC AGGAGTTCAT GATCCTGCCG GTCGGAGCCA AGAACTTCCA
     2041 CGAGGCCATG AGGATCGGCG CCGAGGTCTA CCACAACCTC AAGAACGTCA TCAAGGCCAA
     2101 ATACGGCAAA GACGCCACTA ACGTGGGAGA CGAGGGCGGA TTCGCACCCA ACATCCTCGA
     2161 GAACAACGAG GCTCTGGAGC TGCTGAAATC TGCCATCGAG AAGGCCGGCT ATCCTGACAA
     2221 GATCATCATC GGGATGGACG TCGCCGCGTC CGAGTTCTTC AAGAATGGCA AATACGATCT
     2281 GGACTTCAAG TCACCCGACG ACCCGAAGCG CCACATCACC GGAGATCAGC TGGGAGACTT
     2341 ATACAAGAGC TTCATCAAGA ACTACCCCGT CCAGTCCATC GAGGATCCAT TTGACCAGGA
     2401 TGACTGGGAG AACTGGAGCA AGTTCACGGG CTCAGTGGAC ATCCAGGTGG TGGGAGACGA
     2461 TCTGACCGTC ACCAACCCCA AACGCATCCA GCAGGCATGT GAGAAGAAGG CCTGCAACTG
     2521 CCTGCTGCTG AAGGTCAATC AGATCGGCTC CGTCACCGAG TCCATCCAGG CTTGTAAGCT
     2581 GGCTCAGTCT AACGGTTGGG GTGTGATGGT CAGTCATCGC TCCGGTGAGA CGGAGGACAC
     2641 CTTCATCGCT GACCTGGTGG TCGGTCTCTG CACAGGGCAG ATCAAGACCG GCGCCCCCTG
     2701 CAGATCAGAG CGCTTGGCAA AGTACAACCA GCTGATGAGG ATCGAGGAGG AGCTCGGAGA
     2761 CAAGGCCAAG TTCGCAGGCA AAGACTTCCG CCACCCCAAA CTCTGAGTCG AGCACCACCA
     2821 CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC
     2881 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
     2941 TTTGCTGAAA GGAGGAACTA TATCCGGATT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA
     3001 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA
     3061 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT
     3121 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC
     3181 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT
     3241 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA
     3301 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG
     3361 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA
     3421 TTAACGCTTA CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
     3481 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAA TTAATTCTTA GAAAAACTCA
     3541 TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT TATCAATACC ATATTTTTGA
     3601 AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC AGTTCCATAG GATGGCAAGA
     3661 TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA TACAACCTAT TAATTTCCCC
     3721 TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG TGACGACTGA ATCCGGTGAG
     3781 AATGGCAAAA GTTTATGCAT TTCTTTCCAG ACTTGTTCAA CAGGCCAGCC ATTACGCTCG
     3841 TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC GTGATTGCGC CTGAGCGAGA
     3901 CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG GAATCGAATG CAACCGGCGC
     3961 AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT CAGGATATTC TTCTAATACC
     4021 TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC ATGCATCATC AGGAGTACGG
     4081 ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA GCCAGTTTAG TCTGACCATC
     4141 TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT TCAGAAACAA CTCTGGCGCA
     4201 TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT GCCCGACATT ATCGCGAGCC
     4261 CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA ATCGCGGCCT AGAGCAAGAC
     4321 GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC TGTTTATGTA AGCAGACAGT
     4381 TTTATTGTTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     4441 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     4501 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     4561 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
     4621 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     4681 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     4741 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     4801 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     4861 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     4921 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     4981 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     5041 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     5101 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
     5161 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
     5221 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
     5281 ACACCGCAAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT
     5341 ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG
     5401 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
     5461 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC
     5521 TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG
     5581 CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA
     5641 TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT
     5701 TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG
     5761 ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC
     5821 GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG
     5881 TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG
     5941 CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG
     6001 CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT
     6061 CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA
     6121 CGATCATGCG CACCCGTGGG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC
     6181 GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG
     6241 CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC
     6301 AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG
     6361 CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG
     6421 GCATCGGTCG AGATCCCGGT GCCTAATGAG TGAGCTAACT TACATTAATT GCGTTGCGCT
     6481 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
     6541 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG
     6601 ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG CAAGCGGTCC
     6661 ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG TGGTTAACGG CGGGATATAA
     6721 CATGAGCTGT CTTCGGTATC GTCGTATCCC ACTACCGAGA TATCCGCACC AACGCGCAGC
     6781 CCGGACTCGG TAATGGCGCG CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC
     6841 GCAGTGGGAA CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA
     6901 CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG ATATTTATGC
     6961 CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG GGCCCGCTAA CAGCGCGATT
     7021 TGCTGGTGAC CCAATGCGAC CAGATGCTCC ACGCCCAGTC GCGTACCGTC TTCATGGGAG
     7081 AAAATAATAC TGTTGATGGG TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA
     7141 GTGCAGGCAG CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC
     7201 CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC GACGCCGCTT
     7261 CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT CGGCGCGAGA TTTAATCGCC
     7321 GCGACAATTT GCGACGGCGC GTGCAGGGCC AGACTGGAGG TGGCAACGCC AATCAGCAAC
     7381 GACTGTTTGC CCGCCAGTTG TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC
     7441 GCCGCTTCCA CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG
     7501 GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT TACTGGTTTC
     7561 ACATTCACCA CCCTGAATTG ACTCTCTTCC GGGCGCTATC ATGCCATACC GCGAAAGGTT
     7621 TTGCGCCATT CGATGGTGTC CGGGATCTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG
     7681 GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG
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