LOCUS       pBRE6L2                 5295 bp    DNA     circular      5-OCT-2010
DEFINITION  LMBP 1681.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|567259329|
COMMENT     VNTNAME|pBRE6L2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRE6L2" 0 5295 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 5295 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "TET" 4 0 0 86 1276 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "86..1276") 
COMMENT        #4=(CFSignal (CObList) "ORI" 33 0 1 2350 2752 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2350..2752)") 
COMMENT        #5=(CFSignal (CObList) "constant domain of E6L" 4 0 0 4031 4354 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "variable domain of E6L" 4 0 0 3710 4030 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "leader signal of E6L" 4 0 0 3650 3709 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "5' UTR of E6L" 21 0 0 3612 3649 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of E6L" 21 0 0 4355 4541 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
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COMMENT                   0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pBRE6L2'" 1) 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 10/05/10 12:02" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/05/10 12:02" 1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5295 bp" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 86    End: 1276" 1) 
COMMENT                                   #29=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   86..1276" 1))) "TET") 
COMMENT                            #32=(CFolderPar 
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COMMENT                                        "Start: 3650  End: 3709" 1))) 
COMMENT                                 "leader signal of E6L") 
COMMENT                            #34=(CFolderPar 
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COMMENT                                 "5' UTR of E6L") 
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COMMENT                                        "Start: 4355  End: 4541" 1))) 
COMMENT                                 "3' UTR of E6L"))) "Misc. Feature (2 total)") 
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COMMENT                                        "Start: 2350  End: 2752 (Complementary)" 
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COMMENT                                   #46=(CLinePar 
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FEATURES             Location/Qualifiers
     CDS             86..1276
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(2350..2752)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     misc_feature    4355..4541
                     /vntifkey="21"
                     /label=3'\UTR\of\E6L
     CDS             4031..4354
                     /vntifkey="4"
                     /label=constant\domain\of\E6L
     CDS             3710..4030
                     /vntifkey="4"
                     /label=variable\domain\of\E6L
     CDS             3650..3709
                     /vntifkey="4"
                     /label=leader\signal\of\E6L
     misc_feature    3612..3649
                     /vntifkey="21"
                     /label=5'\UTR\of\E6L
BASE COUNT     1217 a      1472 c      1338 g      1246 t        22 others
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac 
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca 
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg 
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct 
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat 
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt 
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct 
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct 
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa 
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt 
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc 
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca 
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc 
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat 
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg 
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga 
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac 
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg 
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg 
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc 
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg 
     1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca 
     1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga 
     1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa 
     1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt 
     1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc 
     1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt 
     1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac 
     2041 gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac 
     2101 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 
     2161 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 
     2221 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg 
     2281 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 
     2341 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     2401 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     2461 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     2521 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     2581 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     2641 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     2701 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     2761 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     2821 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     2881 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     2941 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
     3001 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     3061 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     3121 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     3181 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     3241 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     3301 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     3361 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     3421 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     3481 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     3541 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     3601 ccattgctgc aggggggggg agtcagcctc acactgatca cacacagaca tgagtgtgcc 
     3661 cactcaggtc ctggggttgc tgctgctgtg gcttacagat gccagatgtg acatccagat 
     3721 gactcagtct ccagcctccc tatccgtttc tgtgggagaa tctgtcacca tcacatgtcg 
     3781 agcaagtgag aatatttaca gtaatttagc atggtatcag cagaaacagg gaaaatctcc 
     3841 tcagctcctt gtctatgttg cgacaaagtt agtagatggt gtgccatcaa ggttcagtgg 
     3901 cagtggatca ggcacacagt attccctcaa gatcaacagc ctgcagtctg aagattttgg 
     3961 gagttattac tgtcaacatt tttgggacac tccattcacg ttcggctcgg ggacaaagtt 
     4021 ggaaatgaaa cgggctgatg ctgcaccaac tgtatccatc ttcccaccat ccagtgagca 
     4081 gttaacatct ggaggtgcct cagtcgtgtg cttcttgaac aacttctacc ccaaagacat 
     4141 caatgtcaag tggaagattg atgggagtga acgacaaaat ggcgtcctga acagttggac 
     4201 tgatcaggac agcaaagaca gcacctacag catgagcagc accctcacgt tgaccaagga 
     4261 cgagtatgaa cgacataaca gctatacctg tgaggccact cacaagacat caacttcacc 
     4321 cattgtcaag agcttcaaca ggaatgagtg ttagagacaa aggtcctgag acgccaccac 
     4381 cagctcccca gctccatcct atcttccctt ctaaggtctt ggaggcttcc ccacaagcga 
     4441 cctaccactg ttgcggtgct ccaaacctcc tccccacctc cttctcctcc tcctcccttt 
     4501 ccttcccccc cccccccccn nnnnnnnnnn nnnnnnnnnn ntgcaggcat cgtggtgtca 
     4561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     4801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 
     4861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     4921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     4981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     5041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     5101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     5161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     5221 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     5281 tttcgtcttc aagaa 
//
