LOCUS       pPLc28BHTNFs            3582 bp    DNA     circular     25-FEB-2011
DEFINITION  LMBP 1708.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pPLc28BHTNFs|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 3582 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "72..173") 
COMMENT        #1=(CFSignal (CObList) 
COMMENT            "5' UTR of the ampicillin resistance gene, incl. RBS" 21 0 0 174 208 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "174..208") 
COMMENT        #2=(CFSignal (CObList) 
COMMENT            "signal sequence of the ampicillin resistance gene" 21 0 0 209 277 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "209..277") 
COMMENT        #3=(CFSignal (CObList) "hTNF" 4 0 0 278 751 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "278..751") 
COMMENT        #4=(CFSignal (CObList) "3' UTR of the human tumor necrosis factor gene" 
COMMENT            21 0 0 752 878 0 (CStringList) (CStringList) 1 1 1 1 "752..878") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 1 1167 1569 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1170..1570)") 
COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2110 2970 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2110..2970)") 
COMMENT        #7=(CFSignal (CObList) "PL promoter" 29 0 0 3336 3466 0 (CStringList) 
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COMMENT                         "DNA 'pPLc28BHTNFs'" 1) 
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COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "length: 3582 bp" 1) 
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COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
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FEATURES             Location/Qualifiers
     promoter        72..173
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
                     /note="E. coli ç-lactamase promoter"
     misc_feature    174..208
                     /vntifkey="21"
                     /label=5'\UTR\of\the\ampicillin\resistance\gene,\incl.\RBS
                     /note="5' UTR of the ampicillin resistance gene, incl. RBS"
     misc_feature    209..277
                     /vntifkey="21"
                     /label=signal\sequence\of\the\ampicillin\resistance\gene
     CDS             278..751
                     /vntifkey="4"
                     /label=hTNF
                     /note="human tumor necrosis factor mature sequence"
     misc_feature    752..878
                     /vntifkey="21"
                     /label=3'\UTR\of\the\human\tumor\necrosis\factor\gene
     rep_origin      complement(1167..1569)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2110..2970)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3336..3466
                     /vntifkey="29"
                     /label=PL\promoter
                     /note="phage ñ major leftward promoter"
BASE COUNT      895 a       940 c       874 g       873 t 
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
      241 tccctttttt gcggcatttt gccttcctgt ttttgcggta cgttcttctt ctcgtacccc 
      301 gagtgacaag cctgtagccc atgttgtagc aaaccctcaa gctgaggggc agctccagtg 
      361 gctgaaccgc cgggccaatg ccctcctggc caatggcgtg gagctgagag ataaccagct 
      421 ggtggtgcca tcagagggcc tgtacctcat ctactcccag gtcctcttca agggccaagg 
      481 ctgcccctcc acccatgtgc tcctcaccca caccatcagc cgcatcgccg tctcctacca 
      541 gaccaaggtc aacctcctct ctgccatcaa gagcccctgc cagagggaga ccccagaggg 
      601 ggctgaggcc aagccctggt atgagcccat ctatctggga ggggtcttcc agctggagaa 
      661 gggtgaccga ctcagcgctg agatcaatcg gcccgactat ctcgactttg ccgagtctgg 
      721 gcaggtctac tttgggatca ttgccctgtg aggaggacga acatccaacc ttcccaaacg 
      781 cctcccctgc cccaatccct ttattacccc ctccttcaga caccctcaac ctcttctggc 
      841 tcaaaaagag aattgggggc ttagggtcgg aacccaagct tggctgcctc gcgcgtttcg 
      901 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
      961 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     1021 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 
     1081 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 
     1141 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     1201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     1261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     1321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     1381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     1441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     1501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     1561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     1621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     1681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     1741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     1801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     1861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     1921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     1981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     2041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     2101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     2161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     2221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     2281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     2341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     2401 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 
     2461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     2521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     2581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     2641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     2701 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt 
     2761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     2821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     2881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     2941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt atgtaagcag 
     3001 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt 
     3061 gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca 
     3121 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact 
     3181 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg 
     3241 gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga cctcagcgcc 
     3301 ggtgatgccg gccacgatgc gtccggcgta gaggatctct cacctaccaa acaatgcccc 
     3361 cctgcaaaaa ataaattcat ataaaaaaca tacagataac catctgcggt gataaattat 
     3421 ctctggcggt gttgacataa ataccactgg cggtgatact gagcacatca gcaggacgca 
     3481 ctgaccacca tgaaggtgac gctcttaaaa ttaagccctg aagaagggca gcattcaaag 
     3541 cagaaggctt tggggtgtgt gatacgaaac gaagcattgg aa 
//
