LOCUS       pBRSTi844               3658 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1814.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRSTi844|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRSTi844" 0 3658 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3658 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "5UTR" 21 0 0 52 80 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "52..80") 
COMMENT        #4=(CFSignal (CObList) "COAT" 4 0 0 81 671 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "81..671") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 0 672 1307 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "672..1307") 
COMMENT        #6=(CFSignal (CObList) "STNVi" 21 0 0 52 1307 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "52..1307") 
COMMENT        #7=(CFSignal (CObList) "ORI" 33 0 1 1640 2050 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1640..2050)") 
COMMENT        #8=(CFSignal (CObList) "AMP" 4 0 1 2590 3450 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2590..3450)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                         "DNA 'pBRSTi844'" 1) 
COMMENT                    #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 3658 bp" 1) 
COMMENT                    #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                                  #27=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "coat protein of STNV" 1) 
COMMENT                                  #28=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   81..671" 1))) "COAT") 
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COMMENT                                       "Start: 2590  End: 3450 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #32=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
COMMENT                                  #33=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #34=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(2590..3450)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Start: 52    End: 80  " 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                  #43=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Satellite tobacco necrosis virus with insertion" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #45=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   52..1307" 1))) "STNVi") 
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COMMENT                                       "Start: 672   End: 1307" 1) 
COMMENT                                  #48=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "3' UTR of STNV RNA" 1) 
COMMENT                                  #49=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       "   672..1307" 1))) "3UTR"))) 
COMMENT                         "Misc. Feature (3 total)") 
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COMMENT                                       "origin plasmid" 1) 
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COMMENT                         "Replication Origin (1 total)"))) "Feature Map")))) 
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COMMENT          #63=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
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FEATURES             Location/Qualifiers
     misc_feature    52..80
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of STNV RNA"
     CDS             81..671
                     /vntifkey="4"
                     /label=COAT
                     /note="coat protein of STNV"
     misc_feature    672..1307
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of STNV RNA"
     misc_feature    52..1307
                     /vntifkey="21"
                     /label=STNVi
                     /note="Satellite tobacco necrosis virus with insertion"
     rep_origin      complement(1640..2050)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(2590..3450)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT      944 a       908 c       911 g       889 t         6 others
ORIGIN
        1 ttccggatcc ggccaagctt ggctctagag gctgcagggg gggggggggg gagtaaagac 
       61 aggaaacttt actgactaac atggcaaaac aacagaacaa caggcgaaaa tccgcaacaa 
      121 tgcgtgcagt gaagcgcatg ataaatacac acttggagca taaaaggttt gcactgatca 
      181 actcagggaa caccaatgca actgctggta cagtacaaaa tctgtccaac ggtataatcc 
      241 aaggagatga tatcaaccag agaagtggtg atcaagtgcg tatagtttca cataaacttc 
      301 acgtacgagg cactgccatc accgtcagcc agacctttag atttatctgg tttcgtgata 
      361 acatgaaccg tgggaccact cccacagttc ttgaggtgtt gaacactgcg aatttcatgt 
      421 cgcagtataa cccaatcacg ttgcagcaaa agagatttac aatactcaag gatgtaactc 
      481 tcaattgttc gctgacaggg gagagcatta aagatcggat aattaacctt ccaggacaac 
      541 tggtgaacta taatggagcg acggctgtag cagcctccaa tggtcccggc gcaatattta 
      601 tgttgcagat tggcgactcc ttggttggtc tgtgggactc ctcttatgag gctgtgtaca 
      661 cagatgcata atcccagagg ttcacaatgt tagtgatggg gcgctgaaag atgcgtagct 
      721 acccttctgg agccacttcc tggtggtaag cagaaatcca agggtacggt ggtacggtgg 
      781 aaagcagtcc cagctctgca ttgggaaccg gcttacaccc agcttagggc taaagtgtac 
      841 tacttgctca tttgtagtct aaatgagacg ttggcctcga cgtgtcgagg tggccnnntc 
      901 catgggaatt ctnnngggat tggaacccct gatggtcgta gtcgaatttc ccgtgtttca 
      961 ttccgagtct cttggtcata atgccattag taggtctagc actcaacgta acttcaaaga 
     1021 tatcctcctt gcaacaagaa tatgtgcgcc gtctgtgttt aaagcggtat attaagtgcg 
     1081 ccggcatatc gttgtttgga ccagggcccc acgccggttg gtacccgggt ggcttcccct 
     1141 cgttcacagg gctttaggag atgataaggt atagttatta gacaaatgcg gacaaacctg 
     1201 aaaagctcgc tagtggtggg ctggccaagc gaagaacctc atccaggtat agttctacat 
     1261 gggaaatttg gtaccatcca aacttctatg aagtcctcga ctaccccaaa cccccccccc 
     1321 cccccccccc cccctgcagg tcgacctcta gagccaagct tggctgcctc gcgcgtttcg 
     1381 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     1441 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     1501 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 
     1561 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 
     1621 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 
     1681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 
     1741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 
     1801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 
     1861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 
     1921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 
     1981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 
     2041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 
     2101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 
     2161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 
     2221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 
     2281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 
     2341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 
     2401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 
     2461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 
     2521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 
     2581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 
     2641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 
     2701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 
     2761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 
     2821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 
     2881 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 
     2941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 
     3001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 
     3061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 
     3121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 
     3181 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt 
     3241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 
     3301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 
     3361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 
     3421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 
     3481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 
     3541 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 
     3601 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa 
//
