ID pTACLSPA4 standard; circular DNA; ; 6662 BP. DE LMBP 1949. CC Accession number: LMBP 1949 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 3-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 515..1048 FT /vntifkey="4" FT /label=lamB\fragment FT /note="E. coli lamB coding sequence encoding amino acids 1 to 178" FT CDS 1757..2563 FT /vntifkey="4" FT /label=lamB\fragment FT /note="E. coli lamB coding sequence encoding amino acids 179 to 446" FT RBS 234..269 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT RBS 270..514 FT /vntifkey="32" FT /label=5'\UTR\of\lamB,\incl.\RBS FT /note="5' UTR containing the RBS of the E. coli lamB gene" FT promoter 199..233 FT /vntifkey="30" FT /label=tac\promoter FT /note="E. coli hybrid tryptophan/lac operon promoter" FT CDS 1058..1225 FT /vntifkey="4" FT /label=spaA FT /note="S. aureus spa coding sequence encoding amino acids 169 to 224" FT CDS 1232..1399 FT /vntifkey="4" FT /label=spaA FT /note="S. aureus spa coding sequence encoding amino acids 169 to 224" FT CDS 1406..1573 FT /vntifkey="4" FT /label=spaA FT /note="S. aureus spa coding sequence encoding amino acids 169 to 224" FT CDS 1580..1747 FT /vntifkey="4" FT /label=spaA FT /note="S. aureus spa coding sequence encoding amino acids 169 to 224" FT misc_feature 2564..2779 FT /vntifkey="21" FT /label=3'\UTR\of\lamB FT promoter complement(6549..6626) FT /vntifkey="30" FT /label=lacIq\promoter FT /note="mutated E. coli repressor lacI promoter, incl. RBS" FT CDS complement(5466..6548) FT /vntifkey="4" FT /label=lacI FT /note="E. coli repressor lacI coding sequence" FT CDS complement(4366..5226) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(3423..3825) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" SQ Sequence 6662 BP; 1596 A; 1543 C; 1497 G; 1436 t; 590 other; ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 60 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 120 tcaaggcgca ctcccgttct gaataatgtt ttttgcgccg acatcataac ggttctggca 180 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg 240 agcggataac aatttcacac aggaaacagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 480 nnnnnnnnnn nnnnnnnnng actcaggaga tagaatgatg attactctgc gcaaacttcc 540 tctggcggtt gccgtcgcag cgggcgtaat gtctgctcag gcaatggctg ttgatttcca 600 cggctatgca cgttccggta ttggttggac aggtagcggc ggtgaacaac agtgtttcca 660 gactaccggt gctcaaagta aataccgtct tggcaacgaa tgtgaaactt atgctgaatt 720 aaaattgggt caggaagtgt ggaaagaggg cgataagagc ttctatttcg acactaacgt 780 ggcctattcc gtcgcacaac agaatgactg ggaagctacc gatccggcct tccgtgaagc 840 aaacgtgcag ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa 900 gcgcttctac caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg 960 tcctggtgcc ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac 1020 ccgctcctct gaagctggtg gttcttcccg ggatcccaat ttcaacaaag aacaacaaaa 1080 tgctttctat gaaatcttga atatgcctaa cttaaacgaa gaacaacgca atggtttcat 1140 ccaaagctta aaagatgacc caagccaaag tgctaaccta ttgtcagaag ctaaaaagtt 1200 aaatgaatct caagcaccga aagcggatcc caatttcaac aaagaacaac aaaatgcttt 1260 ctatgaaatc ttgaatatgc ctaacttaaa cgaagaacaa cgcaatggtt tcatccaaag 1320 cttaaaagat gacccaagcc aaagtgctaa cctattgtca gaagctaaaa agttaaatga 1380 atctcaagca ccgaaagcgg atcccaattt caacaaagaa caacaaaatg ctttctatga 1440 aatcttgaat atgcctaact taaacgaaga acaacgcaat ggtttcatcc aaagcttaaa 1500 agatgaccca agccaaagtg ctaacctatt gtcagaagct aaaaagttaa atgaatctca 1560 agcaccgaaa gcggatccca atttcaacaa agaacaacaa aatgctttct atgaaatctt 1620 gaatatgcct aacttaaacg aagaacaacg caatggtttc atccaaagct taaaagatga 1680 cccaagccaa agtgctaacc tattgtcaga agctaaaaag ttaaatgaat ctcaagcacc 1740 gaaagcggat cccgcctctt tcgccagcaa caatatttat gactatacca acgaaaccgc 1800 gaacgacgtt ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg gcacattaga 1860 actgggtgtc gactacggtc gtgccaactt gcgtgataac tatcgtctgg ttgatggcgc 1920 atcgaaagac ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa 1980 caagtttgtt gttcagtacg ctactgactc gatgacctcg cagggtaaag ggctgtcgca 2040 gggttctggc gttgcatttg ataacgaaaa atttgcctac aatatcaaca acaacggtca 2100 catgctgcgt atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta 2160 cgtgggtatg taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt 2220 cggtattcgc ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta 2280 cgacaacgtc gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc 2340 acaacaatgg caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac 2400 ctacgccaag tgggatgaga aatggggtta cgactacacc ggtaacgctg ataacaacgc 2460 gaacttcggc aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag 2520 cgacgagtgg accttcggtg cccagatgga aatctggtgg taatagcaaa acctgggccg 2580 gataaggcgt ttacgccgca ttcggcaacc aacgcctgat gcgacgcttg cgcgtcttat 2640 caggcctaca acggctgtca aatgtaggcc ggataaggcg tttacgccgc atccggcata 2700 aaaacaggtt gtcattatct gaaggggcga aagcccctct gattatcggg tttagcgcgc 2760 tattgcctgg ctaccgctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2820 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 2940 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3000 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3060 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3120 nnnnnnnnnn nnnnnnnnnc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 3180 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 3240 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 3300 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 3360 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 3420 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3480 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3540 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3600 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3660 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3720 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 3780 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3840 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3900 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3960 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 4020 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 4080 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 4140 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4200 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4260 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 4320 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 4380 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 4440 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 4500 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 4560 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 4620 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 4680 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 4740 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 4800 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 4860 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 4920 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 4980 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 5040 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 5100 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 5160 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 5220 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 5280 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 5340 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 5400 taggcgtatc acgaggccct ttcgtcttca agaattcgcg ctaactcaca ttaattgcgt 5460 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 5520 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc 5580 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag 5640 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt tgacggcggg 5700 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg 5760 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc 5820 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac 5880 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat 5940 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc 6000 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca 6060 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga 6120 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg 6180 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg 6240 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta 6300 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc 6360 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc 6420 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc 6480 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact 6540 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga 6600 aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttcgcc ttcgcgcgcg 6660 aa 6662 //