LOCUS       p220.2                  8952 bp    DNA     circular     19-OCT-2015
DEFINITION  LMBP 1966.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 1966
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 19-OCT-2015
            
COMMENT     VNTNAME|p220.2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "p220.2" 0 8952 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 1 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 8952 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 8 119 0 586 0 1708 0 1767 0 
COMMENT            3496 0 4412 0 6492 0 6894 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 6232 0 6629 0 8951 0 0 
COMMENT            "") #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 4302 0 0 "")) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "EBNA1" 4 0 0 418 2343 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "418..2343") 
COMMENT        #4=(CFSignal (CObList) "Epstein-Barr oriP" 33 0 0 2645 4416 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2645..4416") 
COMMENT        #5=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 5372 5441 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(5372..5441)") 
COMMENT        #6=(CFSignal (CObList) "hygromycin resistance" 4 0 1 5442 6479 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(5442..6479)") 
COMMENT        #7=(CFSignal (CObList) "HSV-TK promoter" 29 0 1 6554 6664 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6554..6658)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 7079 7343 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7884 8744 0 
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COMMENT                          "DNA Plasmid 'p220.2'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 10/19/15 02:55" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8952 bp" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 23-OCT-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Epstein-Barr virus nuclear antigen 1" 1) 
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COMMENT                                 "HSV-TK promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     CDS             418..2343
                     /vntifkey="4"
                     /label=EBNA1
                     /note="Epstein-Barr virus nuclear antigen 1"
     rep_origin      2645..4416
                     /vntifkey="33"
                     /label=Epstein-Barr\oriP
                     /note="Epstein-Barr virus origin of replication (oriP)"
     terminator      complement(5372..5441)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
                     /note="polyA signal of the Herpes simplex thymidine kinase gene"
     CDS             complement(5442..6479)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     promoter        complement(6554..6664)
                     /vntifkey="29"
                     /label=HSV-TK\promoter
     rep_origin      complement(7079..7343)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(7884..8744)
                     /vntifkey="4"
                     /label=ampicillin\resistance
BASE COUNT     2191 a      2206 c      2632 g      1923 t 
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt 
       61 cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc 
      121 gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg 
      181 aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg 
      241 gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca 
      301 gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc 
      361 tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg 
      421 tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct 
      481 ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga 
      541 ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga 
      601 tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc 
      661 tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc aggagcagga 
      721 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca 
      781 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg 
      841 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga 
      901 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca 
      961 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg 
     1021 gcaggagcag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga 
     1081 ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggggcagga 
     1141 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca 
     1201 ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga 
     1261 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga 
     1321 ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg 
     1381 gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt 
     1441 agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg tgaaagagcc 
     1501 agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga aaagaggccc 
     1561 aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc ccctccaggt 
     1621 agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca ccaagaaggt 
     1681 ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc cgcagatgac 
     1741 ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa 
     1801 ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt 
     1861 gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac cgacgaagga 
     1921 acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta caacctaagg 
     1981 cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg tctccccttt 
     2041 ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat tgtctgttat 
     2101 ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc gattaaggac 
     2161 cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg cagctttgac 
     2221 gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc cgcggagggt 
     2281 gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga agggcaggag 
     2341 tgatgtaact tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt cccccgcact 
     2401 aaagaataaa tccccagtag acatcatgcg tgctgttggt gtatttctgg ccatctgtct 
     2461 tgtcaccatt ttcgtcctcc caacatgggg caattgggca tacccatgtt gtcacgtcac 
     2521 tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt acctggtgag 
     2581 caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga atgggagcaa 
     2641 ccagcaggaa aaggacaagc agcgaaaatt cacgccccct tgggaggtgg cggcatatgc 
     2701 aaaggatagc actcccactc tactactggg tatcatatgc tgactgtata tgcatgagga 
     2761 tagcatatgc tacccggata cagattagga tagcatatac tacccagata tagattagga 
     2821 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata taaattagga 
     2881 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga 
     2941 tagcctatgc tacccagata tagattagga tagcatatgc tacccagata tagattagga 
     3001 tagcatatgc tatccagata tttgggtagt atatgctacc cagatataaa ttaggatagc 
     3061 atatactacc ctaatctcta ttaggatagc atatgctacc cggatacaga ttaggatagc 
     3121 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc 
     3181 ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga ttaggatagc 
     3241 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga ttaggatagc 
     3301 atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag cccaccgtgc 
     3361 tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc aggcgcaagt 
     3421 gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc gcccacctac 
     3481 ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt ttgaccgcag 
     3541 tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt ccaaaaccgc 
     3601 agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa aaagagtggc 
     3661 cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt cgggggtgtt 
     3721 agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct tgttacaaat 
     3781 agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc ttaataaccc 
     3841 cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt gtgagatgga 
     3901 catccagtct ttacggcttg tccccacccc atggatttct attgttaaag atattcagaa 
     3961 tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta tattggtgtc 
     4021 atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc gtcacctgaa 
     4081 accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag ttattctatt 
     4141 agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac tgcccgctcc 
     4201 ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg gaatcctgac 
     4261 cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt ccttaggacc 
     4321 cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat gctattgaat 
     4381 tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc cgtttagggt 
     4441 taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg cttatcggta 
     4501 gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc tgggcccctg 
     4561 ggaggtacat gtcccccagc attggtgtaa gagcttcagc caagagttac acataaaggc 
     4621 aatgttgtgt tgcagtccac agactgcaaa gtctgctcca ggatgaaagc cactcagtgt 
     4681 tggcaaatgt gcacatccat ttataaggat gtcaactaca gtcagagaac ccctttgtgt 
     4741 ttggtccccc cccgtgtcac atgtggaaca gggcccagtt ggcaagttgt accaaccaac 
     4801 tgaagggatt acatgcactg ccccgtgacc aatacaaaac aaaagcgctc ctcgtaccag 
     4861 cgaagaaggg gcagagatgc cgtagtcagg tttagttcgt ccggcggcgg gggatcctct 
     4921 agagtcgacc tgcagcccaa gcttggcact ggcgccagaa atccgcgcgg tggtttttgg 
     4981 gggtcggggg tgtttggcag ccacagacgc ccggtgttcg tgtcgcgcca gtacatgcgg 
     5041 tccatgccca ggccatccaa aaaccatggg tctgtctgct cagtccagtc gtggacctga 
     5101 ccccacgcaa cgcccaaaag aataaccccc acgaaccata aaccattccc catgggggac 
     5161 cccgtcccta acccacgggg cccgtggcta tggcgggctt gccgccccga cgttggctgc 
     5221 gagccctggg ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc 
     5281 gtattggccc caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac 
     5341 cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg 
     5401 tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc 
     5461 tcccgatccc cacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag 
     5521 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct 
     5581 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg 
     5641 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc 
     5701 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa 
     5761 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc 
     5821 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag 
     5881 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc 
     5941 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga 
     6001 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt 
     6061 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca 
     6121 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc 
     6181 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca 
     6241 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga 
     6301 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca 
     6361 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg 
     6421 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata 
     6481 tctcattgcc cccggggatc ttgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag 
     6541 ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga cctgggaccg 
     6601 cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt 
     6661 tgtgtcatca tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa 
     6721 cgcgagcaac gggccacggg gatgaagcag cccggcggca cctcgctaac ggattcacca 
     6781 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 
     6841 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcggggc 
     6901 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat 
     6961 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag 
     7021 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcgc 
     7081 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     7141 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     7201 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     7261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     7321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     7381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     7441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     7501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     7561 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     7621 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     7681 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     7741 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     7801 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     7861 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     7921 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     7981 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     8041 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     8101 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     8161 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 
     8221 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     8281 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     8341 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     8401 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     8461 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 
     8521 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     8581 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     8641 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     8701 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     8761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     8821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     8881 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 
     8941 cgtcttcaag aa 
//
