LOCUS       pPLTACHTNFs             3496 bp    DNA     circular     25-FEB-2011
DEFINITION  LMBP 2078.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pPLTACHTNFs|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT        #2=(CFSignal (CObList) "E. coli alkaline phosphatase A signal sequence" 
COMMENT            21 0 0 129 191 0 (CStringList) (CStringList) 1 1 1 1 "129..191") 
COMMENT        #3=(CFSignal (CObList) "hTNF" 4 0 0 192 665 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "192..665") 
COMMENT        #4=(CFSignal (CObList) "3' UTR of hTNF" 21 0 0 666 792 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2024 2884 0 
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COMMENT        #7=(CFSignal (CObList) "PL promoter" 30 0 0 3250 3380 0 (CStringList) 
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COMMENT                         "DNA 'pPLTACHTNFs'" 1) 
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COMMENT                         "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "length: 3496 bp" 1) 
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COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac\promoter
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5'\UTR\containing\the\RBS\of\the\E.\coli\phoA\gene
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=E.\coli\alkaline\phosphatase\A\signal\sequence
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..665
                     /vntifkey="4"
                     /label=hTNF
                     /note="human tumor necrosis factor mature sequence"
     misc_feature    666..792
                     /vntifkey="21"
                     /label=3'\UTR\of\hTNF
                     /note="3' UTR of the human tumor necrosis factor gene"
     rep_origin      complement(1081..1483)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2024..2884)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3250..3380
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="phage ñ major leftward promoter"
BASE COUNT      879 a       935 c       861 g       821 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc ggtacgttct tcttctcgta ccccgagtga caagcctgta gcccatgttg 
      241 tagcaaaccc tcaagctgag gggcagctcc agtggctgaa ccgccgggcc aatgccctcc 
      301 tggccaatgg cgtggagctg agagataacc agctggtggt gccatcagag ggcctgtacc 
      361 tcatctactc ccaggtcctc ttcaagggcc aaggctgccc ctccacccat gtgctcctca 
      421 cccacaccat cagccgcatc gccgtctcct accagaccaa ggtcaacctc ctctctgcca 
      481 tcaagagccc ctgccagagg gagaccccag agggggctga ggccaagccc tggtatgagc 
      541 ccatctatct gggaggggtc ttccagctgg agaagggtga ccgactcagc gctgagatca 
      601 atcggcccga ctatctcgac tttgccgagt ctgggcaggt ctactttggg atcattgccc 
      661 tgtgaggagg acgaacatcc aaccttccca aacgcctccc ctgccccaat ccctttatta 
      721 ccccctcctt cagacaccct caacctcttc tggctcaaaa agagaattgg gggcttaggg 
      781 tcggaaccca agcttggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 
      841 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 
      901 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 
      961 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 
     1021 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 
     1081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     1141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     1201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     1261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     1321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     1381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     1441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     1501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     1561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     1621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     1681 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     1741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     1801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     1861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     1921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     1981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     2041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     2101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     2161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     2221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     2281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     2341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     2401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     2461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     2521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     2581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     2641 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     2701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     2761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     2821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     2881 tcatactctt cctttttcaa tattatgtaa gcagacagtt ttattgttca tgatgatata 
     2941 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata 
     3001 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt 
     3061 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc 
     3121 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca 
     3181 gcaacacctt cttcacgagg cagacctcag cgccggtgat gccggccacg atgcgtccgg 
     3241 cgtagaggat ctctcaccta ccaaacaatg cccccctgca aaaaataaat tcatataaaa 
     3301 aacatacaga taaccatctg cggtgataaa ttatctctgg cggtgttgac ataaatacca 
     3361 ctggcggtga tactgagcac atcagcagga cgcactgacc accatgaagg tgacgctctt 
     3421 aaaattaagc cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg 
     3481 aaacgaagca ttggaa 
//
