ID pET-11a standard; circular DNA; ; 5677 BP. DE LMBP 2290. CC Accession number: LMBP 2290 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-JUL-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(213..260) FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT CDS complement(328..360) FT /vntifkey="4" FT /label=T7\gene\10\fragment FT /note="first 11 codons of phage T7 gene 10" FT RBS complement(361..405) FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT /note="5' UTR of phage T7 gene 10, incl. ribosome binding site" FT promoter complement(407..448) FT /vntifkey="30" FT /label=T7\promoter\with\lac\operator FT /note="phage T7 gene 10 promoter with lac operator" FT promoter 757..834 FT /vntifkey="30" FT /label=lacI\promoter FT /note="E. coli repressor lacI promoter, incl. ribosome binding site" FT CDS 835..1917 FT /vntifkey="4" FT /label=lacI FT /note="E. coli repressor lacI coding sequence" FT rep_origin complement(3666..4068) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4609..5469) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT CDS 3229..3420 FT /vntifkey="4" FT /label=rop FT /note="modulation repressor coding sequence" SQ Sequence 5677 BP; 1299 A; 1550 C; 1496 G; 1332 t; ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300 cgggctttgt tagcagccgg atccgcgacc catttgctgt ccaccagtca tgctagccat 360 atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct 420 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg 480 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 540 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 600 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 660 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 720 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc 780 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 840 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 900 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 960 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 1020 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 1080 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 1140 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 1200 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 1260 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 1320 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 1380 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 1440 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 1500 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 1560 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 1620 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 1680 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 1740 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 1800 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 1860 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1920 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt 1980 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 2040 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 2100 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 2160 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 2220 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 2280 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 2340 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 2400 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat 2460 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 2520 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 2580 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga 2640 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 2700 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg 2760 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc 2820 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc 2880 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt 2940 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc 3000 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga 3060 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc 3120 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc 3180 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag 3240 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag 3300 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac 3360 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 3420 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 3480 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 3540 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 3600 gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 3660 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3720 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3780 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3840 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3900 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3960 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4020 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4080 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4140 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4200 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4260 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4320 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4380 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4440 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4500 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4560 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4620 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4680 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4740 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4800 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4860 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4920 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4980 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5040 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5100 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5160 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5220 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5280 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5340 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5400 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5460 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5520 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5580 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5640 aaataggcgt atcacgaggc cctttcgtct tcaagaa 5677 //