LOCUS       pTrc99A                 4176 bp    DNA     circular     25-SEP-2012
DEFINITION  LMBP 2401.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2401
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 27-SEP-2012
            
COMMENT     VNTNAME|pTrc99A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pTrc99A" 0 4176 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "HaeII" "HincII" "HindIII" "AvaI" "BamHI" "EcoRI" "KpnI" 
COMMENT         "NcoI" "PstI" "SacI" "SalI" "XbaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 3603 3602 1 4176 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 456 0 699 0 1480 0 1853 
COMMENT            0 2223 0 4026 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HincII" "gtyrac" 4 0 5 19 0 130 0 589 0 1524 0 
COMMENT            3409 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4175 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 34 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 29 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 50 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 42 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 55 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 15 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 48 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 17 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 23 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 34 0 0 "")) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "lacI" 4 0 1 363 1445 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(363..1445)") 
COMMENT        #14=(CFSignal (CObList) "lacIq promoter, incl. RBS" 30 0 1 1446 1523 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1446..1523)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 1851 2253 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1851..2253)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2794 3654 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2794..3654)") 
COMMENT        #17=(CFSignal (CObList) "T2 terminator" 43 0 1 3766 3793 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3766..3793)") 
COMMENT        #18=(CFSignal (CObList) "T1 terminator" 43 0 0 3925 3968 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3925..3968") 
COMMENT        #19=(CFSignal (CObList) "trc promoter" 30 0 1 97 140 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(97..140)") 
COMMENT        #20=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 61 96 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(61..96)")) 
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COMMENT        #21=(CFSignal (CObList) "LMBP 2401" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "Last modified: 27-SEP-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pTrc99A'" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2401." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/27/12 04:11" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4176 bp" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Modification Date in the Original DB: 21-SEP-2012" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 2401") 
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COMMENT                                   #47=(CLinePar 
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COMMENT                                        "E. coli repressor lacI coding sequence" 
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COMMENT                                        "E. coli hybrid tryptophan/lacUV5 operon promoter" 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                        "   3925..3968" 1))) "T1 terminator"))) 
COMMENT                          "Terminator (2 total)"))) "Feature Map") 
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COMMENT                                        " N1: 4175 " 1))))) "HindIII: 1 site") 1 
COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                          "CYCGRG" "GRGCYC") 
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COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                          "GAATTC" "CTTAAG") 
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COMMENT                          "GGTACC" "CCATGG") 
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COMMENT                           "GAGCTC" "CTCGAG") 
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COMMENT                           5 1 "RGCGCY" "YCGCGR"))) 
COMMENT                   "Restriction/Methylation Map") 
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COMMENT              1.6976e-313 1.08453e-300) 
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FEATURES             Location/Qualifiers
     CDS             complement(363..1445)
                     /vntifkey="4"
                     /label=lacI
                     /note="E. coli repressor lacI coding sequence"
     promoter        complement(1446..1523)
                     /vntifkey="30"
                     /label=lacIq\promoter,\incl.\RBS
     rep_origin      complement(1851..2253)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2794..3654)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(3766..3793)
                     /vntifkey="43"
                     /label=T2\terminator
     terminator      3925..3968
                     /vntifkey="43"
                     /label=T1\terminator
     promoter        complement(97..140)
                     /vntifkey="30"
                     /label=trc\promoter
                     /note="E. coli hybrid tryptophan/lacUV5 operon promoter"
     RBS             complement(61..96)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
BASE COUNT      990 a      1118 c      1059 g      1009 t 
ORIGIN
        1 cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga attccatggt 
       61 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggatga 
      121 ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa 
      181 aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg atttacgacc 
      241 tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg 
      301 cagtcgatga taagctgtca aaccagatca attcgcgcta actcacatta attgcgttgc 
      361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 
      421 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 
      481 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 
      541 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttga cggcgggata 
      601 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc 
      661 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 
      721 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 
      781 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 
      841 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 
      901 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 
      961 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 
     1021 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 
     1081 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 
     1141 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 
     1201 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 
     1261 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 
     1321 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 
     1381 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 
     1441 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 
     1501 gttttgcacc attcgatggt gtcaacgtaa atgcatgccg cttcgccttc gcgcgcgaat 
     1561 tgatctgctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 
     1621 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 
     1681 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 
     1741 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 
     1801 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 
     1861 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     1921 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     1981 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     2041 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     2101 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     2161 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     2221 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     2281 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     2341 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     2401 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     2461 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     2521 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     2581 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     2641 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     2701 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     2761 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     2821 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     2881 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     2941 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     3001 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     3061 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg taggcatcgt 
     3121 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     3181 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     3241 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     3301 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     3361 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 
     3421 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     3481 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     3541 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     3601 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     3661 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     3721 tgaatgtatt tagaaaaata aacaaaaaga gtttgtagaa acgcaaaaag gccatccgtc 
     3781 aggatggcct tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac 
     3841 cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg 
     3901 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt 
     3961 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc 
     4021 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact 
     4081 gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag 
     4141 gctgaaaatc ttctctcatc cgccaaaaca gccaag 
//
