ID pTrc99A standard; circular DNA; ; 4176 BP. DE LMBP 2401. CC Accession number: LMBP 2401 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 27-SEP-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(363..1445) FT /vntifkey="4" FT /label=lacI FT /note="E. coli repressor lacI coding sequence" FT promoter complement(1446..1523) FT /vntifkey="30" FT /label=lacIq\promoter,\incl.\RBS FT rep_origin complement(1851..2253) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(2794..3654) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(3766..3793) FT /vntifkey="43" FT /label=T2\terminator FT terminator 3925..3968 FT /vntifkey="43" FT /label=T1\terminator FT promoter complement(97..140) FT /vntifkey="30" FT /label=trc\promoter FT /note="E. coli hybrid tryptophan/lacUV5 operon promoter" FT RBS complement(61..96) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS SQ Sequence 4176 BP; 990 A; 1118 C; 1059 G; 1009 t; cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga attccatggt 60 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggatga 120 ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa 180 aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg atttacgacc 240 tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg gtgcaccgtg 300 cagtcgatga taagctgtca aaccagatca attcgcgcta actcacatta attgcgttgc 360 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 420 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt 480 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg 540 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttga cggcgggata 600 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc 660 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc 720 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg 780 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta 840 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg 900 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg 960 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca 1020 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc 1080 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg 1140 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc 1200 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc 1260 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc 1320 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg 1380 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt 1440 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag 1500 gttttgcacc attcgatggt gtcaacgtaa atgcatgccg cttcgccttc gcgcgcgaat 1560 tgatctgctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 1620 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 1680 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 1740 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 1800 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 1860 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1920 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1980 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2040 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2100 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2160 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2220 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2280 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2340 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2400 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2460 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2520 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2580 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 2640 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 2700 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 2760 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 2820 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 2880 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2940 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3000 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3060 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg taggcatcgt 3120 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3180 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 3240 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3300 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3360 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 3420 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 3480 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 3540 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 3600 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 3660 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3720 tgaatgtatt tagaaaaata aacaaaaaga gtttgtagaa acgcaaaaag gccatccgtc 3780 aggatggcct tctgcttaat ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac 3840 cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg gcggatttgt cctactcagg 3900 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt 3960 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc 4020 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact 4080 gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag 4140 gctgaaaatc ttctctcatc cgccaaaaca gccaag 4176 //