ID pEMBLyex4 standard; circular DNA; ; 8800 BP. DE LMBP 2423. CC Accession number: LMBP 2423 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(7735..8595) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(6793..7195) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5535..6338) FT /vntifkey="4" FT /label=URA3 FT /note="S. cerevisiae uracil 3 coding sequence" FT promoter complement(4909..5316) FT /vntifkey="29" FT /label=GAL10/CYC1\promoter FT /note="UAS-G: 5316-5169, GAL10: 5168-5091,CYC1: 5086-4909, (all 3 compl.)" FT misc_feature 4176..4778 FT /vntifkey="21" FT /label=2mu\inverted\repeat FT /note="inverted repeat of 2mu" FT terminator complement(4491..4595) FT /vntifkey="43" FT /label=FLP\terminator FT /note="S. cerevisiae 2mu FLP terminator" FT rep_origin complement(3429..4409) FT /vntifkey="33" FT /label=2mu\ori FT /note="2mu origin, incl. region conferring stability (STB)" FT CDS complement(1977..3071) FT /vntifkey="4" FT /label=defective\LEU2 FT /note="defective LEU2 coding sequence" FT rep_origin complement(754..1209) FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" SQ Sequence 8800 BP; 2494 A; 1952 C; 1893 G; 2456 t; 5 other; ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 60 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 120 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtnta 180 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 240 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 300 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 360 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 420 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 480 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 540 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 600 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 660 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 720 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 780 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 840 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 900 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 960 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 1020 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 1080 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 1140 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 1200 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 1260 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 1320 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 1380 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 1440 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 1500 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 1560 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1620 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 1680 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 1740 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 1800 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 1860 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 1920 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 1980 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 2040 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 2100 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 2160 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 2220 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 2280 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 2340 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 2400 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 2460 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 2520 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 2580 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 2640 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 2700 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 2760 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 2820 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 2880 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 2940 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 3000 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 3060 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 3120 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 3180 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 3240 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 3300 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 3360 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 3420 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 3480 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 3540 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 3600 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 3660 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 3720 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 3780 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 3840 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 3900 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 3960 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 4020 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 4080 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 4140 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 4200 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 4260 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 4320 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 4380 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 4440 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 4500 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 4560 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 4620 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 4680 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 4740 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcatgcc tgcaggtcga 4800 ctctagagga tccccgggta ccgagctcga attgatccgg taatttagtg tgtgtatttg 4860 tgtttgcgtg tctatagaag tatagtaatt tatgctacaa aggacctaat gtataaggaa 4920 agaatattta gagaaaagaa gaaaacaaga gttttatata catacagagc acatgcatgc 4980 catatgatca tgtgtcgtcg cacacatata tatatgcctg tatgtgtcag cactaaagtt 5040 gcctggccat ccacgctata tacacgcctg gcggatctgc tcgaggtcga ttagcctaaa 5100 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta 5160 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc 5220 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 5280 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 5340 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat 5400 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatcgggt 5460 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact 5520 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct 5580 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca 5640 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc 5700 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct 5760 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc 5820 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac 5880 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc 5940 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct 6000 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat 6060 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact 6120 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg 6180 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca 6240 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga 6300 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttccggttt 6360 ttgttctgtg cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata 6420 tgcgtatata taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga 6480 ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa aaaagaataa aaaaaaaatg 6540 atgaattgaa ttgaaaagct aattcgtaat catggtcata gctgtttcct gtgtgaaatt 6600 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6660 gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6720 cgggaaacct gtcgtgccan ctggattaat gaatcggcca acgcgcgggg agaggcggtt 6780 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6840 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6900 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6960 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7020 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7080 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7140 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 7200 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 7260 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 7320 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7380 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7440 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7500 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7560 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7620 gttagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 7680 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7740 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7800 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7860 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7920 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7980 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 8040 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 8100 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 8160 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 8220 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 8280 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 8340 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 8400 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 8460 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 8520 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8580 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8640 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8700 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 8760 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 8800 //