LOCUS       pCAGGS                  4802 bp    DNA     circular      5-NOV-2019
DEFINITION  LMBP 2453.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2453
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 05-NOV-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-13065839|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAGGS|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS" 0 4802 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "NcoI" "SacII" "XhoI" "BglII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 1352 1351 1 4802 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 11 0 4801 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 835 0 3452 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 2 4108 0 4636 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 5 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BglII" "agatct" 2 0 1 91 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 91 182 0 (CStringList) (CStringList) 1 1 1 1 "91..182") 
COMMENT        #6=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            183 539 0 (CStringList) (CStringList) 1 1 1 1 "183..539") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 617 700 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #8=(CFSignal (CObList) "SV40 ori" 33 0 0 842 917 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "841..916") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 948 1022 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 948 1022 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(947..1021)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 1137 1539 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1136..1537)") 
COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2080 2940 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2078..2938)") 
COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3076 3453 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3074..3451") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 3459 4799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3457..4798") 
COMMENT        #15=(CFSignal (CObList) "intron AG promoter" 15 0 0 3828 4751 0 
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COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
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COMMENT        #17=(CFSignal (CObList) "Last modified: 05-NOV-2019" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "NGS data implemented" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4802 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 30-SEP-2013" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Ghent University" 1) 
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COMMENT                          "B-9052 Gent Belgium" 1) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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COMMENT                                 "" 1))) "LMBP 2453") 
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COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2078..2938)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (1 total)") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                        "   183..539" 1))) 
COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #70=(CLinePar 
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COMMENT                                        "   3074..3451" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "   3457..4798" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #80=(CLinePar 
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COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
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COMMENT                                        "   complement(617..699)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 842   End: 917 " 1) 
COMMENT                                   #86=(CLinePar 
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COMMENT                                        "bidirectional origin of SV40" 1) 
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COMMENT                                        "   complement(1136..1537)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                    "Restriction/Methylation Map") 
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FEATURES             Location/Qualifiers
     terminator      91..182
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    183..539
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(617..700)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      842..917
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      948..1022
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(948..1022)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(1137..1539)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(2080..2940)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3076..3453
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        3459..4799
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          3828..4751
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
BASE COUNT     1001 a      1272 c      1341 g      1188 t 
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 
       61 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg 
      121 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt 
      181 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat 
      241 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg 
      301 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct 
      361 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 
      421 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 
      481 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc 
      541 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
      601 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
      661 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
      721 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc 
      781 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
      841 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
      901 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg 
      961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     1021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 
     1081 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     1141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     1201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     1261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     1321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     1381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     1441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     1501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     1561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     1621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     1681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     1741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 
     1801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     1861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     1921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     1981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     2041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     2101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     2161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     2221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     2281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     2341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     2401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     2461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     2521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     2581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     2641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     2701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     2761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     2821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     2881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     2941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     3001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     3061 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 
     3121 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 
     3181 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 
     3241 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 
     3301 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 
     3361 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 
     3421 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 
     3481 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 
     3541 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc 
     3601 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg 
     3661 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa 
     3721 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg 
     3781 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg 
     3841 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc 
     3901 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct 
     3961 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg 
     4021 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg 
     4081 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct 
     4141 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc 
     4201 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc 
     4261 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg 
     4321 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc 
     4381 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 
     4441 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 
     4501 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 
     4561 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 
     4621 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 
     4681 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 
     4741 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 
     4801 aa 
//
