ID pSCGAL10-SN standard; circular DNA; UNA; 9906 BP. DE LMBP 2471. CC Accession number: LMBP 2471 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-MAY-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin complement(754..1209) FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT CDS complement(1977..3071) FT /vntifkey="4" FT /label=defective\LEU2 FT /note="defective S. cerevisiae leucine 2 coding sequence" FT rep_origin complement(3429..4409) FT /vntifkey="33" FT /label=2mu\ori FT /note="S. cerevisiae 2mu plasmid origin, incl. region conferring stability (STB)" FT misc_feature 4176..4778 FT /vntifkey="21" FT /label=2mu\inverted\repeat FT /note="inverted repeat of 2mu" FT terminator complement(4491..4595) FT /vntifkey="43" FT /label=FLP\terminator FT /note="S. cerevisiae 2mu FLP terminator" FT terminator complement(4781..5051) FT /vntifkey="43" FT /label=CYC1\terminator FT /note="S. cerevisiae iso-1-cytochrome C terminator" FT misc_feature complement(5114..5313) FT /vntifkey="21" FT /label=3'\UTR\of\hIFNß FT /note="3' UTR of the human IFNß gene" FT CDS complement(5314..5877) FT /vntifkey="4" FT /label=hIFNß FT /note="signal and mature human IFNß sequence" FT promoter complement(6015..6422) FT /vntifkey="29" FT /label=GAL10/CYC1\promoter FT /note="UAS-G: 6422-6275, GAL10: 6274-6193, CYC1: 6192-6015, (all 3 compl.)" FT CDS complement(6641..7444) FT /vntifkey="4" FT /label=URA3 FT /note="S. cerevisiae uracil 3 coding sequence" FT rep_origin complement(7899..8301) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(8841..9701) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 9906 BP; 2820 A; 2180 C; 2126 G; 2769 t; 11 other; ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 60 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 120 gggtgttggc gggtgtcggg gcgcagccat nnnccannna cgtagcgata gcggagtnta 180 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 240 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggnaattcca acatccaata 300 aatcatacag gcaaggcaaa gaattagcaa aattaagcaa taaagcctca gagcataaag 360 ctaaatcggt tgtaccaaaa acattatgac cctgtaatac ttttgcggga gaagccttta 420 tttcaacgca aggataaaaa tttttagaac cctcatatat tttaaatgca atgcctgagt 480 aatgtgtagg taaagattca aacgggtgag aaaggccgga gacagtcaaa tcaccatcaa 540 tatgatattc aaccgttcta gctgataaat tcatgccgga gagggtagct atttttgaga 600 ggtctacaaa ggctatcagg tcattgcctg agagtctgga gcaaacaaga gaatcgatga 660 acggtaatcg taaaactagc atgtcaatca tatgtacccc ggttgataat cagaaaagcc 720 ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 780 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 840 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 900 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 960 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 1020 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 1080 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 1140 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 1200 agggcgcgta ctatggttgc tttgacgagc acgtataacg tgctttcctc gttagaatca 1260 gagcgggagc taaacaggag gccgattaaa gggattttag acaggaacgg tacgccagaa 1320 tcctgagaag tgtttttata atcagtgagg ccaccgagta aaagagtctg tccatcacgc 1380 aaattaaccg ttgtcgcaat acttctttga ttagtaataa catcacttgc ctgagtagaa 1440 gaactcaaac tatcggcctt gctggtaata tccagaacaa tattaccgcc agccattgca 1500 acaggaaaaa cgctcatgga aatacctaca ttttgacgct caatcgtctg gaattnccat 1560 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 1620 cgccanctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 1680 tcccagtcag tacgttgtaa aacgacggcc agtgccaagc tccttaccac tcttttgtta 1740 ctctattgat ccagctcagc aaaggcagtg tgatctaaga ttctatcttc gcgatgtagt 1800 aaaactagct agaccgagaa agagactaga aatgcaaaag gcacttctac aatggctgcc 1860 atcattatta tccgatgtga cgctgcattt tttttttttt gtaatttcgt gtcgtttcta 1920 ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga atctttttaa 1980 gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa 2040 ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg 2100 gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata 2160 gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccatg gcatggttcg 2220 tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa 2280 cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg 2340 gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca 2400 atcaattgat gttgaacctt caatgtaggg aattcgttct tgatggtttc ctccacagtt 2460 tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat 2520 ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga 2580 acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt tctcttacca 2640 aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt 2700 ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg 2760 gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct aacactaccg 2820 gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt cttggaggct 2880 tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc accaattaaa 2940 tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt aagaacctta 3000 atggcttcgg ctgtgatttc ttgaccaacg tggtcacctg gcaaaacgac gatcttctta 3060 ggggcagaca ttacaatggt atatccttga aatatatata aaaaaaaaaa aaaatgcagc 3120 ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt 3180 tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag 3240 ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta 3300 cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa 3360 tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata 3420 tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc 3480 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag 3540 cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag 3600 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc 3660 gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc 3720 ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct 3780 ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc 3840 tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact 3900 gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct 3960 ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca 4020 ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa 4080 tgtttacatt ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt 4140 tgtctaaaga gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa 4200 gttcaaggag cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag 4260 caaagagata cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg 4320 ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc 4380 aaaagcgctc tgaagttcct atactttcta gctagagaat aggaacttcg gaataggaac 4440 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 4500 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 4560 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 4620 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 4680 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 4740 tccttcaatg ctatcatttc ctttgatatt ggatcataag cttgcaaatt aaagccttcg 4800 agcgtcccaa aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc 4860 tgtacagaaa aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta 4920 taaaaaaata aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag 4980 cggatgtggg gggagggcgt gaatgtaagc gtgacataac taattacatg atatcgacaa 5040 aggaaaaggg ggacggatcc ccgggaattc gagctcgccc ggggatcctc taggcggccg 5100 ctaggcctcg agggactttt gcaccaaaaa taatttattt tccaaaataa aatttaaata 5160 aataaaaata actcataatt taataaaaat ttcaaaatct tctagtgtcc tttcatatgc 5220 agtacattag ccatcagtca cttaaacagc atctgctggt tgaagaatgc ttgaagcaat 5280 tgtccagtcc cagaggcaca ggctaggaga tcttcagttt cggaggtaac ctgtaagtct 5340 gttaatgaag taaaagttcc ttaggatttc cactctgact atggtccagg cacagtgact 5400 gtactccttg gccttcaggt aatgcagaat cctcccataa tatcttttca ggtgcagact 5460 gctcatgagt tttcccctgg tgaaatcttc tttctccagt ttttcttcca ggactgtctt 5520 cagatggttt atctgatgat agacattagc caggaggttc tcaacaatag tctcattcca 5580 gccagtgcta gatgaatctt gtctgaaaat agcaaagatg ttctggagca tctcatagat 5640 ggtcaatgcg gcgtcctcct tctggaactg ctgcagctgc ttaatctcct cagggatgtc 5700 aaagttcatc ctgtccttga ggcagtattc aagcctccca ttcaattgcc acaggagctt 5760 ctgacactga aaattgctgc ttctttgtag gaatccaagc aagttgtagc tcatggaaag 5820 agctgtagtg gagaagcaca acaggagagc aatttggagg agacacttgt tggtcatgtt 5880 cctcgaggcc tttttggcca agctgactct agaggatccc cgggtaccga gctcgaattg 5940 atccggtaat ttagtgtgtg tatttgtgtt tgcgtgtcta tagaagtata gtaatttatg 6000 ctacaaagga cctaatgtat aaggaaagaa tatttagaga aaagaagaaa acaagagttt 6060 tatatacata cagagcacat gcatgccata tgatcatgtg tcgtcgcaca catatatata 6120 tgcctgtatg tgtcagcact aaagttgcct ggccatccac gctatataca cgcctggcgg 6180 atctgctcga ggtcgattag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct 6240 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc 6300 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga 6360 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt 6420 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat 6480 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc 6540 agcgaagcga tgatttttga tcgggtaata actgatataa ttaaattgaa gctctaattt 6600 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc 6660 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc 6720 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat 6780 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg 6840 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa 6900 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc 6960 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct 7020 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt 7080 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt 7140 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg 7200 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca 7260 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct 7320 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa 7380 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg 7440 acatgattta tcttcgtttc cggtttttgt tctgtgcagt tgggttaaga atactgggca 7500 atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt 7560 ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa 7620 tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctaatt cgtaatcatg 7680 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 7740 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc 7800 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccanctgg attaatgaat 7860 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 7920 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 7980 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 8040 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 8100 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 8160 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 8220 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 8280 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 8340 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 8400 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 8460 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 8520 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 8580 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 8640 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 8700 tgacgctcag tggaacgaaa actcacgtta gggattttgg tcatgagatt atcaaaaagg 8760 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 8820 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 8880 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 8940 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 9000 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 9060 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 9120 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 9180 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 9240 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 9300 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 9360 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 9420 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 9480 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 9540 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 9600 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 9660 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 9720 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 9780 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 9840 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 9900 cttcaa 9906 //