LOCUS       pBact-Pcad      9083 bp    DNA     circular UNA 01-APR-2022
DEFINITION  LMBP 2766.
COMMENT     Accession number: LMBP 2766
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 1-APR-2022
FEATURES             Location/Qualifiers
     promoter        341..2471
                     /label=ACTB\promoter
     misc_feature    2472..3466
                     /label=5'\UTR\of\ACTB
     misc_feature    3491..3535
                     /label=5'\UTR\of\mCdh3
     CDS             3536..6004
                     /label=mCdh3
     misc_feature    6005..6678
                     /label=3'\UTR\of\mCdh3
     terminator      6696..6770
                     /label=SV40\polyA\early
     terminator      complement(6696..6770)
                     /label=SV40\polyA\late
     RBS             complement(6867..6904)
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(6905..6988)
                     /label=lac\promoter
     rep_origin      complement(7080..7482)
                     /label=pMB1\ori
     CDS             complement(8023..8883)
                     /label=ampicillin\resistance
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GTTGGGATCT TTGCATTGGC
      421 CCACGGCTCT CAGGATGGGG ATGCTCCCCT TCAGCACCCG GTTCCCCTTG GAAACTGATG
      481 GTCCTGGCTC TGTGGCATGG CAGTGGCACT GTGAGGAGCC CCCTACCAGC AGCACACAGT
      541 GGGGTTGGCA CTGCCACGCT CCGGATGCCG CGCTCTGATC CAACCCCATA ATCAAGGGAA
      601 CCCGAATTGC CCCATCATTG CCCCCACCAC CCCCATCCTG CCGGGCCCTC ACACCCCACG
      661 CTGCCTTCTG GTGACATTCC CCAGCCCAAA CCCACGGCTT CATGGCTACC GCGGGGCATT
      721 TCCCATTGCC GCCCCATTAT CAGCTCTGCA CACCTCCCGC TGTACCCATG CCTCGTGGCT
      781 GCCCTTCTTT GACGTATAAT CTTCTAATTA ATACCCGGCC TTGTCAAAGT GGAGCACAAA
      841 CGTTAATTAA TTCCCCAGCA GGCAGGTAAT TAACAGTGTG ACTCCCTTTT TGCTGCGAGT
      901 GGGGCTGATA CAGAGAGATG TGGCACTATG GAGCCCACGG GGTCCTGGCA CTGGGTGCCC
      961 ACGGAGGTCC CCATGTGCTG CAGTGTCACC GCCTCCGAGG AGACAGTATT GTCCCTGCGG
     1021 TGTCCCTGCA GCTCAGCTCT GTCCACAGGG CCACCTCCAG TTTGGAGGGG ACACAATGCA
     1081 GCCCCGATGC AACCCATCCT CGCAGCATCC CAGGGACAAA GACCCCACTG CAAGACCGCA
     1141 CACAGGGCTG GGTCCCGCTC CCCTAATATC TACAGTGCTT CTTGCATGGC CCCTTAATCA
     1201 ATGCAGTTAA TCAGCATGCG CTCATGCACC GCTCTGGAGC TGCAAAGCCC CTCGCAGCGC
     1261 TGCTCACCAA CACCGCGCAC CGCCCCTGCC CAGCCTGCAG CACGCGCTGC AAACAGGAAA
     1321 GAAACAAAAT ATTGCCCAAA TGTAGGCAAA GGCATTCGGC TGCCTTGACC TCCGCCGGGC
     1381 CGGGCCCTGC CTGACTCAGC TCCTTACTCA GCGCTCGCTT CCTCCCTCCG GCTGCCACCG
     1441 CCGCAGCGCA CACCCTGACA AAGAGTGGCC CTTAACGGGC TCTGAGGTGC ACCCAGCAGT
     1501 GCACTCAGCA GTCCAAGGGC CGGCCTGGAG GTTTGCACCG CTACGTGCTG ACATTAGCAT
     1561 TGAACTTGGC CCTGGGTAGT GCTGCAGGCC GGGCGGGGTG GGTGTAGAGA GTGCAGCGCG
     1621 CGTTGCACCC GGTGCCCCTT CCCCTCCCTT GCATCCCAGC AGGCTGCACC CCAGCACCAG
     1681 GCCCGTGCAT GCATGCTCCT GGTGTTATTG CAGCCTGGTG CATGCATGCG TCTTGATGGT
     1741 GCAGCGCTGT GCATGCATCC TCCTTGGTGT GTAGCAGTCT AGTGCATGCA TACCCCTCGG
     1801 TGTTATTGCT GCTCTGTGCA CGCACGCTCA TTGTATCACT TCATCCCAGT GCATGCACTC
     1861 ACACTGGAGC GATTGCTGCT CGGTGCACGC ACACTCATTG TATCACTGCA GCTCAGTGGC
     1921 TGCACGCACA CCGGTGTTAT TGCTGCTCGG TGCGTGCATG CACATCAGTG TCGCTGCAGC
     1981 TCAGTGCATG CACGCTCATT GCCCATCGCT ATCCCTGCCT CTCCTGCTGG CGCTCCCCGG
     2041 GAGGTGACTT CAAGGGGACC GCAGGACCAC CTCGGGGGTG GGGGGAGGGC TGCACACGCG
     2101 GACCCCGCTC CCCCTCCCCA ACAAAGCACT GTGGAATCAA AAAGGGGGGA GGGGGGATGG
     2161 AGGGGCGCGT CACACCCCCG CCCCACACCC TCACCTCGAG GTGAGCCCCA CGTTCTGCTT
     2221 CACTCTCCCC ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT
     2281 ATTTTGTGCA GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG
     2341 GCGAGGGGCG GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC
     2401 TCCGAAAGTT TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG
     2461 CGCGGCGGGC GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC
     2521 GCCGCCCGCC CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC
     2581 CTTCTCCTCC GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC
     2641 TGCGTGAAAG CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG
     2701 GGTGCGTGCG TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT
     2761 GTGAGCGCTG CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC
     2821 GGCCGGGGGC GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG
     2881 GGTGTGTGCG TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC
     2941 CCTGCACCCC CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC
     3001 GGGGCGTGGC GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG
     3061 CGGGGCGGGG CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC
     3121 GCCGGCGGCT GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG
     3181 AGGGCGCAGG GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC
     3241 GCACCCCCTC TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG
     3301 GGAGGGCCTT CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG
     3361 CCGCAGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT
     3421 GTGACCGGCG GGGTTTATAT CTTCCCTTCT CTGTTCCTCC GCAGCCCCCA AGCTTGATAT
     3481 CGAATTCGGG GAGCGCGCCC CACCGTCGCG GCAGCCTTCT CACCTCCCTT CAGTTATGGA
     3541 GCTTCTTAGT GGGCCTCACG CCTTCCTGCT CCTCCTGCTC CAGGTTTGCT GGCTACGCAG
     3601 CGTGGTCTCC GAGCCCTACC GAGCGGGCTT CATCGGGGAG GCTGGAGTGA CCTTGGAGGT
     3661 GGAAGGAACT GACCTGGAGC CGAGCCAAGT TCTGGGGAAA GTAGCCTTGG CTGGACAGGG
     3721 CATGCACCAT GCAGACAATG GAGACATCAT TATGCTGACT AGGGGGACAG TTCAGGGAGG
     3781 GAAGGATGCG ATGCACTCCC CACCCACCCG CATCTTAAGG AGACGAAAGA GAGAGTGGGT
     3841 GATGCCACCA ATATTCGTCC CCGAGAATGG CAAGGGTCCC TTCCCTCAGA GGCTGAATCA
     3901 GCTCAAATCT AATAAGGACA GAGGCACCAA GATTTTCTAC AGCATCACAG GGCCTGGCGC
     3961 AGACAGTCCC CCCGAAGGAG TCTTCACCAT AGAGAAGGAG TCGGGCTGGC TGTTGTTGCA
     4021 TATGCCACTG GACAGGGAGA AGATTGTCAA GTACGAGCTT TATGGCCACG CTGTATCTGA
     4081 GAATGGTGCC TCTGTAGAGG AGCCCATGAA CATATCCATC ATTGTGACAG ACCAGAATGA
     4141 CAACAAGCCC AAGTTCACTC AAGACACCTT CAGAGGGAGT GTTCTGGAGG GAGTAATGCC
     4201 TGGCACTTCT GTGATGCAGG TGACAGCCAC AGATGAGGAC GATGCTGTCA ACACTTACAA
     4261 TGGGGTGGTG GCTTACTCCA TCCATAGCCA AGAGCCGAAG GAGCCACACG ACCTCATGTT
     4321 CACCATCCAT AAAAGCACGG GAACCATTAG CGTCATATCC AGTGGCCTGG ACCGAGAGAA
     4381 AGTCCCTGAG TACAGACTGA CCGTCCAGGC CACAGACATG GATGGAGAGG GCTCTACCAC
     4441 GACGGCAGAG GCCGTTGTGC AAATCCTTGA TGCCAACGAT AACGCTCCGG AGTTTGAGCC
     4501 GCAGAAGTAT GAGGCTTGGG TGCCTGAGAA CGAAGTGGGC CATGAGGTAC AGAGGCTGAC
     4561 AGTGACTGAT CTCGATGTCC CCAACTCGCC AGCGTGGCGT GCCACCTACC ACATCGTGGG
     4621 AGGTGATGAT GGGGACCATT TCACCATCAC CACTCACCCA GAGACCAACC AAGGCGTCCT
     4681 GACAACCAAG AAGGGTTTGG ATTTTGAGGC TCAGGACCAA CACACCCTGT ATGTAGAAGT
     4741 GACCAACGAG GCTCCCTTTG CAGTGAAGCT CCCGACAGCC ACTGCCACCG TGGTGGTCCA
     4801 TGTGAAAGAT GTCAACGAAG CCCCTGTGTT TGTTCCACCT TCCAAGGTCA TTGAGGCCCA
     4861 GGAAGGCATC TCTATTGGGG AACTGGTCTG CATCTATACC GCACAGGACC CAGACAAGGA
     4921 GGACCAGAAG ATCAGCTACA CCATCTCGAG AGATCCAGCC AACTGGCTTG CTGTGGACCC
     4981 AGACAGTGGT CAGATAACTG CCGCAGGCAT CTTGGATCGT GAGGACGAGC AGTTTGTGAA
     5041 AAACAATGTC TACGAAGTCA TGGTTTTGGC CACAGACAGT GGAAACCCTC CCACCACCGG
     5101 CACTGGGACC CTCCTGCTTA CACTTACTGA CATCAACGAC CATGGCCCGA TCCCTGAACC
     5161 CAGGCAGATC ATCATCTGTA ACCAAAGCCC TGTGCCTCAA GTGCTGAACA TCACTGACAA
     5221 GGACCTGTCC CCCAACTCCT CCCCTTTCCA GGCCCAGCTA ACACATGACT CAGATATCTA
     5281 CTGGATGGCA GAAGTCAGCG AGAAAGGAGA CACCGTGGCC TTGTCCCTGA AGAAGTTCCT
     5341 GAAACAAGAC ACGTATGACT TGCATCTTTC TTTGTCTGAC CATGGCAACA GGGAACAGCT
     5401 AACCATGATC AGGGCCACTG TGTGTGACTG CCATGGCCAA GTGTTCAATG ACTGCCCCAG
     5461 ACCCTGGAAG GGTGGTTTCA TCCTCCCCAT CCTGGGTGCT GTCCTGGCAC TGCTGACCCT
     5521 TCTACTGGCA CTCCTCCTGT TGGTGAGGAA GAAGAGGAAG GTCAAAGAGC CCCTTCTGCT
     5581 CCCAGAAGAT GACACGCGAG ACAATGTCTT CTATTATGGA GAAGAGGGTG GTGGTGAAGA
     5641 GGACCAGGAC TATGACATCA CCCAACTCCA CCGGGGACTG GAGGCCAGGC CTGAGGTGGT
     5701 TCTCCGAAAC GATGTAGTGC CAACCTTCAT CCCCACCCCC ATGTACCGAC CCCGGCCCGC
     5761 CAACCCAGAT GAAATCGGGA ACTTCATCAT CGAGAACCTG AAGGCTGCCA ACACTGACCC
     5821 TACTGCCCCG CCCTACGACT CCCTGCTGGT TTTTGACTAC GAGGGCAGCG GCTCTGATGC
     5881 CGCCTCCCTG AGCTCCCTCA CCACCTCCGC CTCCGACCAG GATCAGGACT ACAACTACCT
     5941 TAACGAGTGG GGAAGTCGAT TCAAGAAACT GGCGGACATG TATGGTGGCG GTGAGGATGA
     6001 CTAGGCTGTC CCTGCCAAGC TGTCCAGGCA AGAACTACAG ACGGGTCACA GCAGCATCTC
     6061 AAGGGAGCCT GGCTCCCGCT CCAGCCAGGA CTCTGAAGTT TGCCCAGAAG TGGCCTGCGT
     6121 GGCACGCTGC ATCTGACCAG AAGGCTGGAC TGTGAGCCTT TCCGATGGAA GGATGTGACT
     6181 CTGACGACAA CACTGATCCC CACCTCAGCC ATGGCTGAGC CCCTCTCAGA GCGGGAATGT
     6241 CTAAAGGTCT CCCGCCTGCC ACACAGCACT CAGCTCTGCC CTCTCAGGGC TTGAACTTGC
     6301 TCCGATGCCC TCGGTGACTT TCCCCACTTG GAATGGCTCC CTCTCTTTAG AAAGAGGCCC
     6361 CTAACTAAGG CCCTGTTTTT CTTTAATGCA GTTTTCAAAA AGAGGCTGGG GTGGGTGGGG
     6421 CAGGGTGCGG ACATCAGTGT CCCCTCAGGC CCTCCAGAAG AGAAGCCTAC AGGGCACTGG
     6481 CCCTGGTACA TTTCTCTGAC ATTTCTTGAG GCCTCACATT GGGGAGGGGT CACTGCCTTT
     6541 TTTTTTTTTT TAATACTGAG TATATCTAAG TTGTCTCGTA TTTTTTTATT TTCCCTGTTA
     6601 TGTGCTGTAG ATGGAGAGTG ATGACAATCG TGTAAATGTA CTAGAATTTT TTTATTAAAA
     6661 TGAACTTTTT TTTTTCCCCC GAATTCCTGC AGCCCAACTT GTTTATTGCA GCTTATAATG
     6721 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
     6781 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC CCCGGGTACC
     6841 GAGCTCGAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
     6901 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
     6961 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
     7021 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
     7081 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
     7141 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
     7201 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
     7261 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     7321 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     7381 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     7441 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     7501 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     7561 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     7621 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     7681 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     7741 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     7801 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
     7861 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
     7921 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
     7981 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
     8041 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
     8101 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
     8161 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
     8221 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
     8281 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
     8341 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
     8401 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
     8461 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
     8521 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
     8581 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
     8641 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
     8701 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
     8761 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
     8821 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
     8881 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG
     8941 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
     9001 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG
     9061 GCGTATCACG AGGCCCTTTC GTC
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