LOCUS       pEX2                    9214 bp    DNA     circular      4-APR-2008
DEFINITION  LMBP 2890.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2890
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-APR-2008
            
COMMENT     VNTNAME|pEX2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEX2" 0 9214 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "EcoRV" "NcoI" "NdeI" "SalI" "SphI" "XbaI" 
COMMENT         "XmnI" "SmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 8078 8077 1 9214 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1114 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1124 0 9213 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 3 1091 0 2274 0 4037 0 0 
COMMENT            "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 2507 0 4054 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 1280 0 3762 0 5574 0 7150 
COMMENT            0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 2209 0 2824 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 1277 0 2457 0 2739 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5937 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 2173 0 5420 0 6307 0 
COMMENT            8819 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SmaI" "cccggg" 4 0 3 1121 0 1516 0 4730 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "ARS1 ori" 33 0 0 1 838 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..838") 
COMMENT        #11=(CFSignal (CObList) "CYC1 terminator" 43 0 1 839 1109 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(839..1109)") 
COMMENT        #12=(CFSignal (CObList) "CYC1 promoter" 29 0 1 1229 2210 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1229..2210)") 
COMMENT        #13=(CFSignal (CObList) "URA3" 4 0 0 3848 4651 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3848..4651") 
COMMENT        #14=(CFSignal (CObList) "2mu ori" 33 0 0 5939 6919 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5939..6919") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 7203 7605 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(7203..7605)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin" 4 0 1 8146 9006 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8146..9006)") 
COMMENT        #17=(CFSignal (CObList) "FLP terminator" 43 0 0 5757 5861 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 2890" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 4-APR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #21=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pEX2" 2) 5 "" 0 4) 
COMMENT              #22=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pEX2'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2890" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/04/08 10:25" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9214 bp" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #31=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #33=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #34=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #35=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #36=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 2890") 
COMMENT                     #37=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #38=(CLinePar (CParagraph 0 (15 #19# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 4-APR-2008"))) 
COMMENT                   "Annotations") 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3848 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3848  End: 4651" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae uracil 3 coding sequence" 
COMMENT                                        1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3848..4651" 1))) "URA3") 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 8146 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 8146  End: 9006 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(8146..9006)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1229  End: 2210 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae cytochrome C1 promoter" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1229..2210)" 1))) 
COMMENT                                 "CYC1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #57=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1     End: 838 " 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae autonomously replicating sequence" 
COMMENT                                        1) 
COMMENT                                   #61=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1..838" 1))) "ARS1 ori") 
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COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5939  End: 6919" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "2\265 origin, incl. region conferring stability (STB)" 
COMMENT                                        1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5939..6919" 1))) "2mu ori") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7203 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7203  End: 7605 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(7203..7605)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 839   End: 1109 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae iso-1-cytochrome C terminator" 
COMMENT                                        1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(839..1109)" 1))) 
COMMENT                                 "CYC1 terminator") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5757 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5757  End: 5861" 1))) 
COMMENT                                 "FLP terminator"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #81=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #82=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #83=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1114 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
COMMENT                     #85=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #86=(CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 5937 " 1))))) "XbaI: 1 site") 1 5 
COMMENT                          "TCTAGA" "AGATCT") 
COMMENT                     #88=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
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COMMENT                                  (CObjectList 
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COMMENT                                        " N1: 1124 " 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 9213 " 1))))) "EcoRI: 2 sites") 1 
COMMENT                          5 "GAATTC" "CTTAAG") 
COMMENT                     #92=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #93=(CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 2507 " 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 4054 " 1))))) "NcoI: 2 sites") 1 5 
COMMENT                          "CCATGG" "GGTACC") 
COMMENT                     #96=(CRSFolderPar 
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COMMENT                                        " N1: 2209 " 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 2824 " 1))))) "SalI: 2 sites") 1 5 
COMMENT                          "GTCGAC" "CAGCTG") 
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COMMENT                             (CObjectList 
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COMMENT                                           " N1: 1091 " 1) 
COMMENT                                     #103=(CLinePar 
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COMMENT                                           " N2: 2274 " 1) 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 4037 " 1))))) "EcoRV: 3 sites") 
COMMENT                           3 3 "GATATC" "CTATAG") 
COMMENT                     #105=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 3 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
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COMMENT                                    (CObjectList 
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COMMENT                                           " N1: 1277 " 1) 
COMMENT                                     #108=(CLinePar 
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COMMENT                                           " N2: 2457 " 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 2739 " 1))))) "SphI: 3 sites") 
COMMENT                           5 1 "GCATGC" "CGTACG") 
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COMMENT                                           " N1: 1121 " 1) 
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COMMENT                                           " N2: 1516 " 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #9# 3) 1 2 2 0 191) 
COMMENT                                           " N3: 4730 " 1))))) "SmaI: 3 sites") 
COMMENT                           3 3 "CCCGGG" "GGGCCC") 
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COMMENT                                           " N1: 1280 " 1) 
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COMMENT                                           " N2: 3762 " 1) 
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COMMENT                                           " N3: 5574 " 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 7150 " 1))))) "NdeI: 4 sites") 
COMMENT                           2 4 "CATATG" "GTATAC") 
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COMMENT                                           " N3: 6307 " 1) 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #8# 4) 1 2 2 0 191) 
COMMENT                                           " N4: 8819 " 1))))) "XmnI: 4 sites") 
COMMENT                           5 5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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COMMENT                1 2 "?" 0) 
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COMMENT                "@N (@H%)" 0) 
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COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
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COMMENT                "@N" 0) 
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COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #133=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
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FEATURES             Location/Qualifiers
     rep_origin      1..838
                     /vntifkey="33"
                     /label=ARS1\ori
                     /note="S. cerevisiae autonomously replicating sequence"
     terminator      complement(839..1109)
                     /vntifkey="43"
                     /label=CYC1\terminator
                     /note="S. cerevisiae iso-1-cytochrome C terminator"
     promoter        complement(1229..2210)
                     /vntifkey="29"
                     /label=CYC1\promoter
                     /note="S. cerevisiae cytochrome C1 promoter"
     CDS             3848..4651
                     /vntifkey="4"
                     /label=URA3
                     /note="S. cerevisiae uracil 3 coding sequence"
     rep_origin      5939..6919
                     /vntifkey="33"
                     /label=2mu\ori
                     /note="2µ origin, incl. region conferring stability (STB)"
     rep_origin      complement(7203..7605)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(8146..9006)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     terminator      5757..5861
                     /vntifkey="43"
                     /label=FLP\terminator
BASE COUNT     2496 a      2102 c      2098 g      2518 t 
ORIGIN
        1 ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta 
       61 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca 
      121 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg 
      181 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc 
      241 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt 
      301 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta 
      361 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca 
      421 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca 
      481 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca 
      541 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag 
      601 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa 
      661 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga 
      721 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa 
      781 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct 
      841 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt 
      901 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc 
      961 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc 
     1021 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta 
     1081 attacatgat atcgacaaag gaaaaggggg acggatcccc gggaattcag ttattattaa 
     1141 tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg 
     1201 acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat 
     1261 acagagcaca tgcatgccat atgatcatgt gtcgtcgcac acatatatat atgcctgtat 
     1321 gtgtcagcac taaagttgcc tggccatcca cgctatatac acgcctggcg gatctgctcg 
     1381 aggattgcct acgcgtgggc ttgatccacc aaccaacgct cgccaaatga gctggcgctt 
     1441 ggtcttcggt catcgtccgt aaaccccggc caaagagacc ggaaagatcg gtgaaaacat 
     1501 cttgatcttg ctcccgggaa ttttagattc aggtaggaaa ttgattacat aaatactgtt 
     1561 accctgaatc atattcgacg atgtcgtctc acacggaaat ataattcatt tcttggtttt 
     1621 ccaaaaaaat tttcattttt tttcactttt ttgtttcgtc ctcctttttt ttttttttta 
     1681 ttttttttcc tgtgttcacc tttttttttt ttttttttca gtttacaact ttctgcattc 
     1741 ttttctgtgt tttttttttt ttcgtttttc catttgttcg ttcgttgcct gtttttttgc 
     1801 cctattgttc tcgagcctaa aaattttttc ctttcctgct ttcctttctt cgttcaaagt 
     1861 ttcctattcc attgttctct tcggtaaact cattgttgct cggaactcag atatattcag 
     1921 gtcaatttac tgtacttcaa ttgacttttt tcttgaaatt tcaacttgcc tttcaacttg 
     1981 ttcttctttt ttaattctta ttctacactt tagttccctt accttgttcc taattattgt 
     2041 ctgcaaaaga aaacatacac ctatttcatt cacacactaa aacatgtctg acgaagaaca 
     2101 caacctttga aaatgctgac gctggtgcct ccgccaccta cccaatgcaa tgttctgcct 
     2161 tgagaaagaa tggtttcgtt gtcatcaaag gtagaccatg taagattgtc gacatgtcca 
     2221 cttccaagac cggtaagcac ggtcacgcca aggtccattt ggttaccctt gatatcttca 
     2281 ctggtaagaa gttggaagat ttgtctccat ccactcacaa cttggaagtt ccatttgtca 
     2341 agagaagcga ataccaattg ttggatattg atgatggcta cttgtccttg atgaccatga 
     2401 acggtgaaac caaagacgat gtcaacgcac cagaaggtag gttgggtgac agcatgcaag 
     2461 ctgctttcga tgaaggtaag gatttgatgg tcactatcat ttctgccatg ggtgaagaag 
     2521 ctgccatctc cttcaaggaa gctccaagat cctctacgcc ggacgcatcg tggccggcat 
     2581 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 
     2641 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 
     2701 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 
     2761 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 
     2821 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg 
     2881 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 
     2941 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 
     3001 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg 
     3061 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc 
     3121 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat 
     3181 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt 
     3241 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc 
     3301 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 
     3361 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg 
     3421 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc 
     3481 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac 
     3541 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg 
     3601 gggggggggg gggggggggg gagcttttca attcaattca tcattttttt tttattcttt 
     3661 tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga 
     3721 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac 
     3781 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag 
     3841 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc 
     3901 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt 
     3961 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact 
     4021 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa 
     4081 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg 
     4141 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat 
     4201 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga 
     4261 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc 
     4321 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt 
     4381 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt 
     4441 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag 
     4501 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt 
     4561 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc 
     4621 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat 
     4681 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg 
     4741 tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaagc tccccccccc 
     4801 cccccccccc ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt 
     4861 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga 
     4921 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat 
     4981 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca 
     5041 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat 
     5101 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc 
     5161 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa 
     5221 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc 
     5281 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct 
     5341 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac 
     5401 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc aggactaatg 
     5461 ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc 
     5521 agataacagc aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc 
     5581 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc 
     5641 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga 
     5701 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac 
     5761 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac 
     5821 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt 
     5881 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag 
     5941 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca 
     6001 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca 
     6061 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc 
     6121 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg 
     6181 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga 
     6241 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga 
     6301 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca 
     6361 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa 
     6421 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt 
     6481 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca 
     6541 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat 
     6601 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag 
     6661 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc 
     6721 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag 
     6781 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc 
     6841 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat 
     6901 gaagcacaga tgcttcgttc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 
     6961 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 
     7021 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 
     7081 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 
     7141 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 
     7201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     7261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 
     7321 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     7381 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     7441 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     7501 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     7561 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     7621 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     7681 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     7741 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     7801 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
     7861 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     7921 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     7981 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     8041 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 
     8101 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 
     8161 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 
     8221 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 
     8281 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 
     8341 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 
     8401 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 
     8461 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 
     8521 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 
     8581 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 
     8641 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 
     8701 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 
     8761 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 
     8821 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 
     8881 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 
     8941 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 
     9001 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     9061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     9121 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 
     9181 taggcgtatc acgaggccct ttcgtcttca agaa 
//
