ID pEX2 standard; circular DNA; ; 9214 BP. DE LMBP 2890. CC Accession number: LMBP 2890 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 4-APR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin 1..838 FT /vntifkey="33" FT /label=ARS1\ori FT /note="S. cerevisiae autonomously replicating sequence" FT terminator complement(839..1109) FT /vntifkey="43" FT /label=CYC1\terminator FT /note="S. cerevisiae iso-1-cytochrome C terminator" FT promoter complement(1229..2210) FT /vntifkey="29" FT /label=CYC1\promoter FT /note="S. cerevisiae cytochrome C1 promoter" FT CDS 3848..4651 FT /vntifkey="4" FT /label=URA3 FT /note="S. cerevisiae uracil 3 coding sequence" FT rep_origin 5939..6919 FT /vntifkey="33" FT /label=2mu\ori FT /note="2µ origin, incl. region conferring stability (STB)" FT rep_origin complement(7203..7605) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(8146..9006) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT terminator 5757..5861 FT /vntifkey="43" FT /label=FLP\terminator SQ Sequence 9214 BP; 2496 A; 2102 C; 2098 G; 2518 t; ttccacatgt taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta 60 agtgcactag ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca 120 tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg 180 gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc 240 gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt 300 tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta 360 catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca 420 agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca 480 ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca 540 aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag 600 atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa 660 atactactca gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga 720 gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa cgccatttaa 780 tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa aatgtaagct 840 tgcaaattaa agccttcgag cgtcccaaaa ccttctcaag caaggttttc agtataatgt 900 tacatgcgta cacgcgtctg tacagaaaaa aaagaaaaat ttgaaatata aataacgttc 960 ttaatactaa cataactata aaaaaataaa tagggaccta gacttcaggt tgtctaactc 1020 cttccttttc ggttagagcg gatgtggggg gagggcgtga atgtaagcgt gacataacta 1080 attacatgat atcgacaaag gaaaaggggg acggatcccc gggaattcag ttattattaa 1140 tttagtgtgt gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg 1200 acctaatgta taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat 1260 acagagcaca tgcatgccat atgatcatgt gtcgtcgcac acatatatat atgcctgtat 1320 gtgtcagcac taaagttgcc tggccatcca cgctatatac acgcctggcg gatctgctcg 1380 aggattgcct acgcgtgggc ttgatccacc aaccaacgct cgccaaatga gctggcgctt 1440 ggtcttcggt catcgtccgt aaaccccggc caaagagacc ggaaagatcg gtgaaaacat 1500 cttgatcttg ctcccgggaa ttttagattc aggtaggaaa ttgattacat aaatactgtt 1560 accctgaatc atattcgacg atgtcgtctc acacggaaat ataattcatt tcttggtttt 1620 ccaaaaaaat tttcattttt tttcactttt ttgtttcgtc ctcctttttt ttttttttta 1680 ttttttttcc tgtgttcacc tttttttttt ttttttttca gtttacaact ttctgcattc 1740 ttttctgtgt tttttttttt ttcgtttttc catttgttcg ttcgttgcct gtttttttgc 1800 cctattgttc tcgagcctaa aaattttttc ctttcctgct ttcctttctt cgttcaaagt 1860 ttcctattcc attgttctct tcggtaaact cattgttgct cggaactcag atatattcag 1920 gtcaatttac tgtacttcaa ttgacttttt tcttgaaatt tcaacttgcc tttcaacttg 1980 ttcttctttt ttaattctta ttctacactt tagttccctt accttgttcc taattattgt 2040 ctgcaaaaga aaacatacac ctatttcatt cacacactaa aacatgtctg acgaagaaca 2100 caacctttga aaatgctgac gctggtgcct ccgccaccta cccaatgcaa tgttctgcct 2160 tgagaaagaa tggtttcgtt gtcatcaaag gtagaccatg taagattgtc gacatgtcca 2220 cttccaagac cggtaagcac ggtcacgcca aggtccattt ggttaccctt gatatcttca 2280 ctggtaagaa gttggaagat ttgtctccat ccactcacaa cttggaagtt ccatttgtca 2340 agagaagcga ataccaattg ttggatattg atgatggcta cttgtccttg atgaccatga 2400 acggtgaaac caaagacgat gtcaacgcac cagaaggtag gttgggtgac agcatgcaag 2460 ctgctttcga tgaaggtaag gatttgatgg tcactatcat ttctgccatg ggtgaagaag 2520 ctgccatctc cttcaaggaa gctccaagat cctctacgcc ggacgcatcg tggccggcat 2580 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 2640 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 2700 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 2760 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 2820 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg 2880 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 2940 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 3000 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg 3060 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc 3120 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat 3180 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt 3240 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc 3300 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 3360 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg 3420 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc 3480 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac 3540 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg 3600 gggggggggg gggggggggg gagcttttca attcaattca tcattttttt tttattcttt 3660 tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga 3720 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac 3780 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag 3840 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgttgc 3900 tgccaagcta tttaatatca tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt 3960 tcgtaccacc aaggaattac tggagttagt tgaagcatta ggtcccaaaa tttgtttact 4020 aaaaacacat gtggatatct tgactgattt ttccatggag ggcacagtta agccgctaaa 4080 ggcattatcc gccaagtaca attttttact cttcgaagac agaaaatttg ctgacattgg 4140 taatacagtc aaattgcagt actctgcggg tgtatacaga atagcagaat gggcagacat 4200 tacgaatgca cacggtgtgg tgggcccagg tattgttagc ggtttgaagc aggcggcaga 4260 agaagtaaca aaggaaccta gaggcctttt gatgttagca gaattgtcat gcaagggctc 4320 cctatctact ggagaatata ctaagggtac tgttgacatt gcgaagagcg acaaagattt 4380 tgttatcggc tttattgctc aaagagacat gggtggaaga gatgaaggtt acgattggtt 4440 gattatgaca cccggtgtgg gtttagatga caagggagac gcattgggtc aacagtatag 4500 aaccgtggat gatgtggtct ctacaggatc tgacattatt attgttggaa gaggactatt 4560 tgcaaaggga agggatgcta aggtagaggg tgaacgttac agaaaagcag gctgggaagc 4620 atatttgaga agatgcggcc agcaaaacta aaaaactgta ttataagtaa atgcatgtat 4680 actaaactca caaattagag cttcaattta attatatcag ttattacccg ggaatctcgg 4740 tcgtaatgat ttttataatg acgaaaaaaa aaaaattgga aagaaaaagc tccccccccc 4800 cccccccccc ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt 4860 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga 4920 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat 4980 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca 5040 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat 5100 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc 5160 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa 5220 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc 5280 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct 5340 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac 5400 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc aggactaatg 5460 ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg tatactcatc 5520 agataacagc aatacctgat cactacttcg cactagtttc tcggtactat gcatatgatc 5580 caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc 5640 atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga 5700 taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac 5760 gcatttaagc ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac 5820 acgcagatat aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt 5880 cggaagcgct cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag 5940 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca 6000 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca 6060 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc 6120 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg 6180 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga 6240 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga 6300 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca 6360 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa 6420 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt 6480 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca 6540 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat 6600 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag 6660 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc 6720 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag 6780 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc 6840 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat 6900 gaagcacaga tgcttcgttc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 6960 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 7020 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 7080 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 7140 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 7200 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7260 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7320 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 7380 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7440 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7500 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7560 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7620 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 7680 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7740 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7800 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7860 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7920 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7980 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8040 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8100 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8160 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8220 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8280 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 8340 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 8400 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8460 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8520 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8580 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8640 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8700 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8760 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8820 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8880 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8940 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9000 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9060 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9120 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9180 taggcgtatc acgaggccct ttcgtcttca agaa 9214 //