LOCUS       pBRsE6HsE6L             6365 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2963.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6HsE6L|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6HsE6L" 0 6365 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1684625219 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6365 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 31 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "31..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "HINGE" 4 0 0 843 908 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..908") 
COMMENT        #9=(CFSignal (CObList) "CH2" 4 0 0 909 1238 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "909..1238") 
COMMENT        #10=(CFSignal (CObList) "CH3" 4 0 0 1239 1562 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1239..1562") 
COMMENT        #11=(CFSignal (CObList) "3UTR" 21 0 0 1563 1653 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1563..1653") 
COMMENT        #12=(CFSignal (CObList) "tac" 30 0 0 1683 1717 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1683..1717") 
COMMENT        #13=(CFSignal (CObList) "5UTR" 21 0 0 1718 1780 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1718..1780") 
COMMENT        #14=(CFSignal (CObList) "sPHOA" 21 0 0 1781 1843 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1781..1843") 
COMMENT        #15=(CFSignal (CObList) "VL" 4 0 0 1844 2163 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1844..2163") 
COMMENT        #16=(CFSignal (CObList) "CL" 4 0 0 2164 2488 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2164..2488") 
COMMENT        #17=(CFSignal (CObList) "3UTR" 21 0 0 2489 2657 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2489..2657") 
COMMENT        #18=(CFSignal (CObList) "ORI" 33 0 1 4355 4755 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4355..4755)") 
COMMENT        #19=(CFSignal (CObList) "AMP" 4 0 1 5297 6157 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5297..6157)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
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COMMENT              #21=(CRefLinePar 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pBRsE6HsE6L'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6365 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #34=(CFolderPar 
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COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #38=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #39=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #40=(CLinePar 
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COMMENT                                        "   192..551" 1))) "VH") 
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COMMENT                                        "Start: 552   End: 842 " 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 1 of E6H" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   552..842" 1))) "CH1") 
COMMENT                            #46=(CFolderPar 
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COMMENT                                  (CParagraph 843 (3 #8# 0) 1 2 2 0 327) 
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COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 843   End: 908 " 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "hinge domain of E6H" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "   843..908" 1))) "HINGE") 
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COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 909   End: 1238" 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 2 of E6H" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   909..1238" 1))) "CH2") 
COMMENT                            #56=(CFolderPar 
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COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1239  End: 1562" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain 3 of E6H" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #60=(CLinePar 
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COMMENT                                        "   1239..1562" 1))) "CH3") 
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COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1844  End: 2163" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6L" 1) 
COMMENT                                   #64=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1844..2163" 1))) "VL") 
COMMENT                            #66=(CFolderPar 
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COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2164  End: 2488" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "constant domain of E6L" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
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COMMENT                                        "   2164..2488" 1))) "CL") 
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COMMENT                                        "Start: 5297  End: 6157 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5297..6157)" 1))) "AMP"))) 
COMMENT                          "CDS (8 total)") 
COMMENT                     #76=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                        "Start: 1563  End: 1653" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "3' UTR 0F E6H" 1) 
COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
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COMMENT                                        "   1563..1653" 1))) "3UTR") 
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COMMENT                                   #93=(CLinePar 
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COMMENT                                        "Start: 1718  End: 1780" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
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COMMENT                                   #95=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
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COMMENT                                        "   1718..1780" 1))) "5UTR") 
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COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1781  End: 1843" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
COMMENT                                   #100=(CLinePar 
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COMMENT                                         "   1781..1843" 1))) "sPHOA") 
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COMMENT                                          "Start: 2489  End: 2657" 1) 
COMMENT                                    #104=(CLinePar 
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COMMENT                                          "3' UTR of E6L" 1) 
COMMENT                                    #105=(CLinePar 
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COMMENT                                    #106=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "   2489..2657" 1))) "3UTR"))) 
COMMENT                          "Misc. Feature (6 total)") 
COMMENT                     #107=(CFolderPar 
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COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 31    End: 65  " 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                           1) 
COMMENT                                     #111=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   31..65" 1))) "tac") 
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COMMENT                                           "Start: 1683  End: 1717" 1) 
COMMENT                                     #115=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "E. coli hybrid tryptophan/lac operon promoter" 
COMMENT                                           1) 
COMMENT                                     #116=(CLinePar 
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COMMENT                                     #117=(CLinePar 
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COMMENT                                           "   1683..1717" 1))) "tac"))) 
COMMENT                           "Promoter Prokaryotic (2 total)") 
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COMMENT                                           "Start: 4355  End: 4755 (Complementary)" 
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COMMENT                                     #121=(CLinePar 
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COMMENT                                           "origin plasmid" 1) 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #123=(CLinePar 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="constant domain 1 of E6H"
     CDS             843..908
                     /vntifkey="4"
                     /label=HINGE
                     /note="hinge domain of E6H"
     CDS             909..1238
                     /vntifkey="4"
                     /label=CH2
                     /note="constant domain 2 of E6H"
     CDS             1239..1562
                     /vntifkey="4"
                     /label=CH3
                     /note="constant domain 3 of E6H"
     misc_feature    1563..1653
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR 0F E6H"
     promoter        1683..1717
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    1718..1780
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    1781..1843
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             1844..2163
                     /vntifkey="4"
                     /label=VL
                     /note="variable domain of E6L"
     CDS             2164..2488
                     /vntifkey="4"
                     /label=CL
                     /note="constant domain of E6L"
     misc_feature    2489..2657
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of E6L"
     rep_origin      complement(4355..4755)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(5297..6157)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1584 a      1756 c      1560 g      1465 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctacact atgagcagct cagtgactgt cccctccagc acctggccaa 
      781 gccagaccgt tacctgcagt gttgctcacc cagccagcag caccacggtg gacaaaaaac 
      841 ttgagcccag cgggcccact tcaacaatca acccctgtcc tccatgcaag gagtgtcaca 
      901 aatgcccagc tcctaacctc gagggtggac catccgtctt catcttccct ccaaatatca 
      961 aggatgtact catgatctcc ctgacaccca aggtcacgtg tgtggtggtg gatgtgagcg 
     1021 aggatgaccc agacgtccag atcagctggt ttgtgaacaa cgtggaagta ctcacagctc 
     1081 agacacaaac ccatagagag gattacaaca gtactatccg ggtggtcagt gccctcccca 
     1141 tccagcacca ggactggatg agtggcaagg agttcaaatg caaggtcaac aacaaagacc 
     1201 tcccagcgcc cattgagaga accatctcaa aaattaaagg gatagtcaga gctccacaag 
     1261 tatacatctt gtcgccacca ccagagcagt tgtccaggaa agatgtcagt ctgacttgcc 
     1321 tggccgtggg cttcagccct gaagacatca gtgtggagtg gaccagcaat gggcatacag 
     1381 aggagaacta caaggacacc gcaccagtcc tagactctga cggttcttac ttcatatata 
     1441 gcaagctcaa tatgaaaaca agcaagtggg agaaaacaga ttccttctca tgcaacgtga 
     1501 gacacgaggg tctgaaaaat tactacctga agaagaccat ctcccggtct ccgggtaaat 
     1561 gagctcagca cccacaaagc tctcaggtcc taagagacac tggcacccat atccatgcat 
     1621 cccttgtata aataaagcat ccagcaaagc ctgctagagt cgagcttact ccccatcccc 
     1681 ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc 
     1741 acacaggaaa caggatccgc ggatccgtgg agaaaataaa gtgaaacaaa gcactattgc 
     1801 actggcactc ttaccgttac tgtttacccc tgtgacaaaa gcggacatcc agatgactca 
     1861 gtctccagcc tccctatccg tttctgtggg agaatctgtc accatcacat gtcgagcaag 
     1921 tgagaatatt tacagtaatt tagcatggta tcagcagaaa cagggaaaat ctcctcagct 
     1981 ccttgtctat gttgcgacaa agttagtaga tggtgtgcca tcaaggttca gtggcagtgg 
     2041 atcaggcaca cagtattccc tcaagatcaa cagcctgcag tctgaagatt ttgggagtta 
     2101 ttactgtcaa catttttggg acactccatt cacgttcggc tcggggacaa agttggaaat 
     2161 gaaacgggct gatgctgcac caactgtatc catcttccca ccatccagtg agcagttaac 
     2221 atctggaggt gcctcagtcg tgtgcttctt gaacaacttc taccccaaag acatcaatgt 
     2281 caagtggaag attgatggga gtgaacgaca aaatggcgtc ctgaacagtt ggactgatca 
     2341 ggacagcaaa gacagcacct acagcatgag cagcaccctc acgttgacca aggacgagta 
     2401 tgaacgacat aacagctata cctgtgaggc cactcacaag acatcaactt cacccattgt 
     2461 caagagcttc aacaggaatg agtgttagag acaaaggtcc tgagacgcca ccaccagctc 
     2521 cccagctcca tcctatcttc ccttctaagg tcttggaggc ttccccacaa gcgacctacc 
     2581 actgttgcgg tgctccaaac ctcctcccca cctccttctc ctcctcctcc ctttccttcc 
     2641 cccccccccc ccccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg 
     2701 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact 
     2761 cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag 
     2821 cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc 
     2881 cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat 
     2941 ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt 
     3001 ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct 
     3061 gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac 
     3121 cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg cctcggcgag 
     3181 cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc 
     3241 gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg gcggcacctc 
     3301 gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat 
     3361 gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg 
     3421 cggcgcatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg 
     3481 ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata 
     3541 cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga 
     3601 atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc 
     3661 attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct 
     3721 gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat 
     3781 accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc 
     3841 cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc 
     3901 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt 
     3961 atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag 
     4021 gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg 
     4081 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 
     4141 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 
     4201 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta 
     4261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 
     4321 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 
     4381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     4441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     4501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     4561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     4621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     4681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     4741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     4801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     4861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     4921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 
     4981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     5041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     5101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     5161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 
     5221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 
     5281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 
     5341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 
     5401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 
     5461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 
     5521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 
     5581 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt 
     5641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 
     5701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 
     5761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 
     5821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 
     5881 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca 
     5941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 
     6001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 
     6061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 
     6121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 
     6181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 
     6241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 
     6301 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc 
     6361 aagaa 
//
