LOCUS       pBRsE6Hy3f2             4849 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2965.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBRsE6Hy3f2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRsE6Hy3f2" 0 4849 
COMMENT       (CDBMol 0 0 1 1 1 0 0 268786312 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4849 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "tac" 30 0 0 32 65 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "32..65") 
COMMENT        #4=(CFSignal (CObList) "5UTR" 21 0 0 66 128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "66..128") 
COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "129..191") 
COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 842 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..842") 
COMMENT        #8=(CFSignal (CObList) "H1-4" 4 0 0 843 1028 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "843..1028") 
COMMENT        #9=(CFSignal (CObList) "CH2f" 4 0 0 1029 1124 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1029..1124") 
COMMENT        #10=(CFSignal (CObList) "MOUSE" 21 0 0 192 731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "192..731") 
COMMENT        #11=(CFSignal (CObList) "HUMAN" 21 0 0 732 1124 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "732..1124") 
COMMENT        #12=(CFSignal (CObList) "ORI" 33 0 1 2840 3240 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2840..3240)") 
COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 3781 4641 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3781..4641)")) (CObList) 
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COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pBRsE6Hy3f2'" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4849 bp" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 192   End: 551 " 1) 
COMMENT                                   #32=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "variable domain of E6H" 1) 
COMMENT                                   #33=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #34=(CLinePar 
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COMMENT                                        "   192..551" 1))) "VH") 
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COMMENT                                        "chimeric constant domain 1 of the heavy chain" 
COMMENT                                        1) 
COMMENT                                   #38=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                   #42=(CLinePar 
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COMMENT                                        "hinge domain of human \3543" 1) 
COMMENT                                   #43=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #44=(CLinePar 
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COMMENT                            #45=(CFolderPar 
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COMMENT                                        "Start: 1029  End: 1124" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "fragment of CH2 of the human \3543 heavy chain" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Start: 3781  End: 4641 (Complementary)" 
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COMMENT                                   #52=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
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COMMENT                          "CDS (5 total)") 
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COMMENT                                        "Start: 66    End: 128 " 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
COMMENT                                        1) 
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COMMENT                                        "Start: 129   End: 191 " 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli alkaline phosphatase A signal sequence" 
COMMENT                                        1) 
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COMMENT                                   #68=(CLinePar 
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COMMENT                          "Misc. Feature (4 total)") 
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COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
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COMMENT                          "Replication Origin (1 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     promoter        32..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..842
                     /vntifkey="4"
                     /label=CH1
                     /note="chimeric constant domain 1 of the heavy chain"
     CDS             843..1028
                     /vntifkey="4"
                     /label=H1-4
                     /note="hinge domain of human ì3"
     CDS             1029..1124
                     /vntifkey="4"
                     /label=CH2f
                     /note="fragment of CH2 of the human ì3 heavy chain"
     misc_feature    192..731
                     /vntifkey="21"
                     /label=MOUSE
                     /note="mouse part of E6Hy3f2"
     misc_feature    732..1124
                     /vntifkey="21"
                     /label=HUMAN
                     /note="human part of E6Hy3f2"
     rep_origin      complement(2840..3240)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3781..4641)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1144 a      1362 c      1219 g      1124 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctactcc ctcagcagcg tggtgaccgt gccctccagc agcttgggca 
      781 cccagaccta cacctgcaac gtgaatcaca agcccagcaa caccaaggtg gacaagagag 
      841 ttgagctcaa aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccagagccca 
      901 aatcttgtga cacacctccc ccgtgcccac ggtgcccaga gcccaaatct tgtgacacac 
      961 ctcccccatg cccacggtgc ccagagccca aatcttgtga cacacctccc ccgtgcccaa 
     1021 ggtgcccagc acctgaactc ttgggaggac cgtcagtctt cctcttcccc ccaaaaccca 
     1081 aggataccct tatgatttcc cggacccctg acttagttaa ctaagcttag ttaactaagt 
     1141 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 
     1201 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 
     1261 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc 
     1321 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc 
     1381 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg 
     1441 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt 
     1501 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat 
     1561 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc 
     1621 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg 
     1681 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca 
     1741 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca 
     1801 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt 
     1861 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca 
     1921 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag 
     1981 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa 
     2041 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc 
     2101 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct 
     2161 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct 
     2221 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac 
     2281 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc 
     2341 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat 
     2401 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat 
     2461 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat 
     2521 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg 
     2581 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 
     2641 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 
     2701 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 
     2761 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
     2821 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     2881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     2941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     3001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     3061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     3121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     3181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     3241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     3301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     3361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     3421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     3481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     3541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     3601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     3661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     3721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     3781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     3841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     3901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     3961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     4021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     4081 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     4141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     4201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     4261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     4321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     4381 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 
     4441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     4501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     4561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     4621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     4681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     4741 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 
     4801 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa 
//
