ID pGMCSFlacZ standard; circular DNA; UNA; 9291 BP. DE LMBP 2979. CC Accession number: LMBP 2979 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-MAY-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT misc_feature 1..1082 FT /vntifkey="21" FT /label=5'\flanking\region\of\mCsf2 FT /note="5' flanking region of the mouse Csf2 gene" FT promoter 1083..2222 FT /vntifkey="29" FT /label=mCsf2\promoter FT /note="Mouse granulocyte-macrophage colony stimulatingfactor 2 promoter" FT CDS 2467..5694 FT /vntifkey="4" FT /label=lacZ FT /note="modified N-terminal fragment of the lacZ gene start of the XGPRT fragment at position 2467, start of the tryptophanyl t-RNA synthetase at position 2559, start of lacZ at position 2645" FT terminator 5958..6032 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(5958..6032) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT promoter complement(6128..6144) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(6208..6291) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin complement(6383..6785) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7326..8186) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(8625..9080) FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 9244..9260 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" SQ Sequence 9291 BP; 2218 A; 2407 C; 2451 G; 2215 t; ttctgcctcc ctgaagaatg ttgatcatca ggatgggtca gaccatctta aatgctcatg 60 tgtctgtccc gtgggatgag agggactttg gggcccattt ccttccctcc tggtgttgac 120 ttcctcttgt gataaagatc ctggggcagc cacacgcttc tggttccccc agggcccatg 180 tcaagggaca ctccccggaa actccttcca gagggttctc tcctggcctt gtgggctggg 240 aggccaaaga ggggtcactc cacccatctg gtccccacct ccgccctgcc acagccccac 300 tgaggccctt gagtcagcaa ggctgtctga tgctattgcc gcttctttcc atgcgtgcaa 360 tctaggaatg cctgcccgtg tcaccgcctc caccctgatg tcatctttct aggagaaaga 420 tacatatcta cccacaaact gcatccccaa gcccttagaa tcctgcccca taagtgacaa 480 ctttggcacc tgtagaccat atccttgacg tgagtcttga ctctggggcc tctgctttcc 540 tcttagccac caccctgcca cacgcttggg ctaagacgaa tgcctttagt tcttcaacat 600 ggccattctg gccccccagt ctctctttct acatgattcc tgtggtcttg tcctgagttc 660 cacctgttat ctgaccctcg aaagccctca cttctgtctg gtttcatcag tccactgaat 720 cagcttcttg ggccctaaag catgtttctt ggctaggggc cccctctcat atcaagaggg 780 caccgccaga agcccaaggg ttctcacaaa gcaggcaaaa caagtgtggc tcatgttaga 840 tgcaccctta tgtaaaacct cctccacctt gacatgttcc tatgagtaca tatatatcca 900 acaccaccag catacacaca tgactgaaga gatacacata tgtatcccca tggtctcaaa 960 cttcctgtat aggaaggctt gggccagatc ccttgggcaa acacaaactc gtgccgtctt 1020 ctccaaaagc cattgtgaaa atcaaacact tgttagcccc ttgagaccat gctgcctttc 1080 cagaagcagt tcctgattcc acagagagct tctggagagg gaggtgggct ggagcaaggg 1140 gctgtgcaac agactaagcc tgggagaact tgccagggaa gcgggggttg gggcgcacgt 1200 gtgtgtgtgt gtgtgtgtgt gtgtgtgcgc gcgcgtgcgt gtgtgcgtgc ctggttattg 1260 tgttacagct agttactatg ttacagccag cctcagagac ccaggtatcc cataatggta 1320 cagatagcag taatgtgtcc tttttgaatg gcaggctgct ctgaagaggg taaagaggct 1380 cacataactc aaaggaagag tcgcttggca aagggggagg gcagaatcag cagtcaaatg 1440 ggagtagcct tacctgcccc agccagaagc tctagccatg ctgtgaattt ttaggattga 1500 acatgccttt gtaactggga ttgtagcagt cagttgggat ggtatcataa gagaacatgg 1560 tatagtctgc ctggcttcta tacccattgc tgtcattctc actgctccca aggtggcagg 1620 ggtggggctt ggctgggcag tttgcccaga atggtctcct cagtgggagt ctgtggagac 1680 cattatagaa ttgtgtcatt gggctggacc ttatttaata gataatgagg tggacttgtg 1740 agaagtgtat atctcagaag gtggctggaa agagaacggg ccaggactgg ggctggaatg 1800 agccaccaga gtaggtagag cttgcccaaa ggcctccagg aacagcaggt gctatggaag 1860 caagagcccc actcagtatc tcccaaaccc cgccccagcc actccaggcc aggaaatcca 1920 aatatgcctg gaggcccctc aaaaaggaga ggctagccag aggctgggtc agactgccca 1980 ggcagggtgg gaaaggcctt taatcagccc gcaggtgggc tgccagttct tggaagggct 2040 tattaatgaa aaccccccaa gcctgacaac ctgggggaag gctcactggc cccatgtata 2100 gctgataagg gccaggagat tccacaactc aggtagttcc cccgcccccc tggagttctg 2160 tggtcaccat taatcatttc ctctaactgt gtatataaga gctcttttgc agtgagccca 2220 gtgacctgca ggcatgcaag cttggacaca agacaggctt gcgagatatg tttgagaata 2280 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata 2340 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact 2400 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc 2460 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa 2520 tggaaaggca ttattgccgt aagccgtggc ggtctggtac cggtgggtga agaccagaaa 2580 cagcacctcg aactgagccg cgatattgcc cagcgtttca acgcgctgta tggcgagatc 2640 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 2700 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 2760 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca 2820 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc 2880 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc 2940 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca 3000 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt 3060 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt 3120 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg 3180 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc 3240 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat 3300 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg 3360 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag 3420 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc 3480 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg 3540 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa 3600 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc 3660 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc 3720 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc 3780 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc 3840 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg 3900 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc 3960 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct 4020 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat 4080 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg 4140 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta 4200 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt 4260 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc 4320 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg 4380 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg 4440 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt 4500 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat 4560 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt 4620 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg 4680 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc 4740 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc 4800 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat 4860 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca 4920 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt 4980 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg 5040 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg 5100 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg 5160 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt 5220 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc 5280 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat 5340 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat 5400 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat 5460 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa 5520 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc 5580 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa 5640 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa 5700 ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa 5760 aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc 5820 tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg 5880 ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg 5940 ctttctgaca aactcggaac ttgtttattg cagcttataa tggttacaaa taaagcaata 6000 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 6060 aactcatcaa tgtatcttat catgtctgga tccccgaggg ggggcccggt acccagcttt 6120 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct 6180 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 6240 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 6300 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 6360 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 6420 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 6480 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 6540 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 6600 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 6660 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 6720 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 6780 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 6840 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 6900 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 6960 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 7020 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7080 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 7140 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 7200 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 7260 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 7320 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 7380 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 7440 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 7500 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 7560 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 7620 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 7680 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 7740 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 7800 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 7860 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 7920 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 7980 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 8040 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 8100 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 8160 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 8220 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 8280 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 8340 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 8400 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 8460 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 8520 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 8580 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgaaattg taaacgttaa 8640 tattttgtta aaattcgcgt taaatatttg ttaaatcagc tcatttttta accaataggc 8700 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 8760 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 8820 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 8880 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 8940 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 9000 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 9060 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 9120 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 9180 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa 9240 ttgtaatacg actcactata gggcgaattg gccaagtcgg ccgagctcga a 9291 //