LOCUS       pTRKH1                 10948 bp    DNA     circular     21-OCT-2011
DEFINITION  LMBP 3034.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3034
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-OCT-2011
            
            
COMMENT     VNTNAME|pTRKH1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            6422 0 9591 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoNI" "cctnnnnnagg" 6 0 1 7024 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4904 0 10947 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1993 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 25 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 6585 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 6 1083 0 1337 0 3104 0 
COMMENT            5542 0 9205 0 9459 0 0 "")) 
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COMMENT        #9=(CFSignal (CObList) "pAMB1 ori" 33 0 0 1993 4908 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "p15A ori" 33 0 1 5675 6174 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "repD" 4 0 0 2580 2879 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pTRKH1'" 1) 
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FEATURES             Location/Qualifiers
     CDS             601..1338
                     /vntifkey="4"
                     /label=erythromycin\resistance
     rep_origin      1993..4908
                     /vntifkey="33"
                     /label=pAMB1\ori
                     /note="part of pAMbeta1 containing replication origin, incl. repD and repE"
     rep_origin      complement(5675..6174)
                     /vntifkey="33"
                     /label=p15A\ori
                     /note="E. coli p15A origin"
     CDS             6483..7673
                     /vntifkey="4"
                     /label=tetracycline\resistance
     CDS             8723..9460
                     /vntifkey="4"
                     /label=erythromycin\resistance
     CDS             2580..2879
                     /vntifkey="4"
                     /label=repD
     CDS             2922..4412
                     /vntifkey="4"
                     /label=repE
BASE COUNT     3486 a      2178 c      2331 g      2953 t 
ORIGIN
        1 ttcccgggga tccgtcgacc tgcagccaag cttatcgatt ctagactcga gagctctcga 
       61 gtctagaatc gatacgattt tgaagtggca acagataaaa aaaagcagtt taaaattgtt 
      121 gctgaacttt taaaacaagc aaatacaatc attgtcgcaa cagatagcga cagagaaggc 
      181 gaaaacattg cctggtcgat cattcataaa gcaaatgcct tttctaaaga taaaacgtat 
      241 aaaagactat ggatcaatag tttagaaaaa gatgtgatcc gtagcggttt tcaaaatttg 
      301 caaccaggaa tgaattacta tcccttttat caagaagcgc aaaagaaaaa cgaaatgata 
      361 caccaatcag tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt cgtgctgact 
      421 tgcaccatat cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa gaagttatgg 
      481 aaataagact tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt 
      541 ataataggaa ttgaagttaa attagatgct aaaaatttgt aattaagaag gagtgattac 
      601 atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa 
      661 ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa 
      721 gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac 
      781 agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt 
      841 caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggagt 
      901 attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct 
      961 gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca 
     1021 ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa 
     1081 tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca 
     1141 gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga 
     1201 gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta 
     1261 aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta 
     1321 tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac 
     1381 taaagggaat gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag 
     1441 gtccctagcg ctcttatcat ggggaagctc ggatcatatg caagacaaaa taaactcgca 
     1501 acagcacttg gagaaatggg acgaatcgag aaaaccctct ttacgctgga ttacatatct 
     1561 aataaagccg taaggagacg ggttcaaaaa ggtttaaata aaggagaagc aatcaatgca 
     1621 ttagctagaa ctatattttt tggacaacgt ggagaattta gagaacgtgc tctccaagac 
     1681 cagttacaaa gagctagtgc actaaacata attattaacg ctataagtgt gtggaacact 
     1741 gtatatatgg aaaaagccgt agaagaatta aaagcaagag gagaatttag agaagattta 
     1801 atgccatatg cgtggccgtt aggatgggaa catatcaatt ttcttggaga atacaaattt 
     1861 gaaggattac atgacactgg gcaaatgaat ttacgtcctt tacgtataaa agagccgttt 
     1921 tattcttaat ataacggctc tttttataga aaaaatcctt agcgtggttt ttttccgaaa 
     1981 tgctggcggt accccaagaa ttagaaatga gtagatcaaa ttattcacga atagaatcag 
     2041 gaaaatcaga tccaaccata aaaacactag aacaaattgc aaagttaact aactcaacgc 
     2101 tagtagtgga tttaatccca aatgagccaa cagaaccaga gccagaaaca gaatcagaac 
     2161 aagtaacatt ggatttagaa atggaagaag aaaaaagcaa tgacttcgtg tgaataatgc 
     2221 acgaaatcgt tgcttatttt tttttaaaag cggtatacta gatataacga aacaacgaac 
     2281 tgaatagaaa cgaaaaaaga gccatgacac atttataaaa tgtttgacga cattttataa 
     2341 atgcatagcc cgataagatt gccaaaccaa cgcttatcag ttagtcagat gaactcttcc 
     2401 ctcgtaagaa gttatttaat taactttgtt tgaagacggt atataaccgt actatcatta 
     2461 tatagggaaa tcagagagtt ttcaagtatc taagctactg aatttaagaa ttgttaagca 
     2521 atcaatcgga aatcgtttga ttgctttttt tgtattcatt tatagaaggt ggagtttgta 
     2581 tgaatcatga tgaatgtaaa acttatataa aaaatagttt attggagata agaaaattag 
     2641 caaatatcta tacactagaa acgtttaaga aagagttaga aaagagaaat atctacttag 
     2701 aaacaaaatc agataagtat ttttcttcgg agggggaaga ttatatatat aagttaatag 
     2761 aaaataacaa aataatttat tcgattagtg gaaaaaaatt gacttataaa ggaaaaaaat 
     2821 ctttttcaaa acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa gttaattaaa 
     2881 caacctattt tataggattt ataggaaagg agaacagctg aatgaatatc ccttttgttg 
     2941 tagaaactgt gcttcatgac ggcttgttaa agtacaaatt taaaaatagt aaaattcgct 
     3001 caatcactac caagccaggt aaaagcaaag gggctatttt tgcgtatcgc tcaaaatcaa 
     3061 gcatgattgg cggtcgtggt gttgttctga cttccgagga agcgattcaa gaaaatcaag 
     3121 atacatttac acattggaca cccaacgttt atcgttatgg aacgtatgca gacgaaaacc 
     3181 gttcatacac gaaaggacat tctgaaaaca atttaagaca aatcaatacc ttctttattg 
     3241 attttgatat tcacacggca aaagaaacta tttcagcaag cgatatttta acaaccgcta 
     3301 ttgatttagg ttttatgcct actatgatta tcaaatctga taaaggttat caagcatatt 
     3361 ttgttttaga aacgccagtc tatgtgactt caaaatcaga atttaaatct gtcaaagcag 
     3421 ccaaaataat ttcgcaaaat atccgagaat attttggaaa gtctttgcca gttgatctaa 
     3481 cgtgtaatca ttttggtatt gctcgcatac caagaacgga caatgtagaa ttttttgatc 
     3541 ctaattaccg ttattctttc aaagaatggc aagattggtc tttcaaacaa acagataata 
     3601 agggctttac tcgttcaagt ctaacggttt taagcggtac agaaggcaaa aaacaagtag 
     3661 atgaaccctg gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa aagggtttaa 
     3721 tagggcgtaa taacgtcatg tttaccctct ctttagccta ctttagttca ggctattcaa 
     3781 tcgaaacgtg cgaatataat atgtttgagt ttaataatcg attagatcaa cccttagaag 
     3841 aaaaagaagt aatcaaaatt gttagaagtg cctattcaga aaactatcaa ggggctaata 
     3901 gggaatacat taccattctt tgcaaagctt gggtatcaag tgatttaacc agtaaagatt 
     3961 tatttgtccg tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt caacgtgttc 
     4021 atttgtcaga atggaaagaa gatttaatgg cttatattag cgaaaaaagc gatgtataca 
     4081 agccttattt agtgacgacc aaaaaagaga ttagagaagt gctaggcatt cctgaacgga 
     4141 cattagataa attgctgaag gtactgaagg cgaatcagga aattttcttt aagattaaac 
     4201 caggaagaaa tggtggcatt caacttgcta gtgttaaatc attgttgcta tcgatcatta 
     4261 aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct gacaaattct tttgacttag 
     4321 agcatacatt cattcaagag actttaaaca agctagcaga acgccctaaa acggacacac 
     4381 aactcgattt gtttagctat gatacaggct gaaaataaaa cccgcactat gccattacat 
     4441 ttatatctat gatacgtgtt tgttttttct ttgctgttta gcgaatgatt agcagaaata 
     4501 tacagagtaa gattttaatt aattattagg gggagaagga gagagtagcc cgaaaacttt 
     4561 tagttggctt ggactgaacg aagtgaggga aaggctacta aaacgtcgag gggcagtgag 
     4621 agcgaagcga acacttgatt ttttaatttt ctatctttta taggtcatta gagtatactt 
     4681 atttgtccta taaactattt agcagcataa tagatttatt gaataggtca tttaagttga 
     4741 gcatattaga ggaggaaaat cttggagaaa tatttgaaga acccgattac atggattgga 
     4801 ttagttcttg tggttacgtg gtttttaact aaaagtagtg aatttttgat ttttggtgtg 
     4861 tgtgtcttgt tgttagtatt tgctagtcaa agtgattaaa tagaattccg gatgagcatt 
     4921 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 
     4981 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 
     5041 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 
     5101 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa 
     5161 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg 
     5221 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc 
     5281 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc 
     5341 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag 
     5401 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg 
     5461 gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac 
     5521 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt 
     5581 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg 
     5641 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat 
     5701 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc 
     5761 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga 
     5821 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc 
     5881 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc 
     5941 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc 
     6001 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga 
     6061 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa 
     6121 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag 
     6181 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa 
     6241 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga 
     6301 tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt 
     6361 agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgtttga cagcttatca 
     6421 tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt 
     6481 gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag 
     6541 gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca 
     6601 gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg 
     6661 cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc 
     6721 tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcctct 
     6781 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 
     6841 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 
     6901 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 
     6961 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 
     7021 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag 
     7081 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 
     7141 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 
     7201 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 
     7261 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 
     7321 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 
     7381 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 
     7441 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 
     7501 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 
     7561 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 
     7621 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 
     7681 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 
     7741 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 
     7801 cagcagccgc acgcggcgca tctcgggacc ccttgtagga aaatgtccta agtgtggcaa 
     7861 caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac 
     7921 tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga 
     7981 gggaaaagaa accctggtaa aaggaaatca aaaacaaaga gaaaaagccc tacaatgccg 
     8041 ttgtaaaaat tggggaaaag ggatatattg attttatatc tttctcaaaa taaacataaa 
     8101 agccctttaa agagggcttt tatatattaa tcacaaatca cttatcacaa atcacttatc 
     8161 acaaatcaca agtgatttgt gattgttgat gataaaataa gaataagaag aaatagaaag 
     8221 aagtgagtga ttgtgggaaa tttaggcgca caaaaagaaa aacgaaatga tacaccaatc 
     8281 agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga cttgcaccat 
     8341 atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat ggaaataaga 
     8401 cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg 
     8461 aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg 
     8521 gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt 
     8581 gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt 
     8641 tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg 
     8701 taatcacttc aggagagatt acatgaacaa aaatataaaa tattctcaaa actttttaac 
     8761 gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt 
     8821 ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca 
     8881 ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact 
     8941 gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca 
     9001 gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt 
     9061 ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg 
     9121 taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca 
     9181 attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat 
     9241 aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt 
     9301 tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca 
     9361 agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc aagtattgtc 
     9421 tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt cgctttttta 
     9481 aatttggaaa gttacacgtt actaaaggga atggagataa attattagat atactactga 
     9541 cagcttccaa gaagctaaag aggtccctag cgcctacggg gaatttgtat cgataagggg 
     9601 tacaaattcc cactaagcgc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg 
     9661 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg 
     9721 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag 
     9781 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc 
     9841 ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac 
     9901 tatgactgag agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg 
     9961 caccgctgtc cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta 
    10021 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc 
    10081 cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc 
    10141 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga 
    10201 agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat 
    10261 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac 
    10321 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg 
    10381 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc 
    10441 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa 
    10501 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 
    10561 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct 
    10621 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 
    10681 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca 
    10741 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 
    10801 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 
    10861 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 
    10921 gctcaccgtc tttcattgcc atacggaa 
//
