LOCUS       pJB3                    4788 bp    DNA     circular     26-JAN-2010
DEFINITION  LMBP 3042.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3042
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 26-JAN-2010
            
            
COMMENT     VNTNAME|pJB3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 538 0 3805 0 4360 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 2223 0 4034 0 4693 0 
COMMENT            0 "") 
COMMENT        #3=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 3 567 0 1841 0 3555 0 
COMMENT            0 "") 
COMMENT        #4=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 2 577 0 2330 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 185 0 507 0 2338 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "RsaI" "gtac" 3 0 6 405 0 648 0 1205 0 1268 0 
COMMENT            1571 0 3915 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SspI" "aatatt" 4 0 2 2681 0 4239 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "224..307") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 308 345 0 
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COMMENT        #12=(CFSignal (CObList) "Tn5 neo promoter" 30 0 1 2301 2350 0 
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COMMENT        #13=(CFSignal (CObList) "RK2 ori" 33 0 1 2590 3114 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3361 4221 0 
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COMMENT                          "DNA Plasmid 'pJB3'" 1) 
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COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                                        "P382 coding sequence; P285 starts at 1901" 
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COMMENT                          "GAATTC" "CTTAAG") 
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FEATURES             Location/Qualifiers
     promoter        224..307
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     RBS             308..345
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
                     /note="5' UTR of the E. coli lacZ gene, incl. ribosome binding site"
     CDS             346..576
                     /vntifkey="4"
                     /label=lacZ\fragment
     CDS             complement(1044..2192)
                     /vntifkey="4"
                     /label=trfA
                     /note="P382 coding sequence; P285 starts at 1901"
     promoter        complement(2301..2350)
                     /vntifkey="30"
                     /label=Tn5\neo\promoter
                     /note="Tn5 neomycin resistance gene promoter"
     rep_origin      complement(2590..3114)
                     /vntifkey="33"
                     /label=RK2\ori
                     /note="RK2 vegetative replication origin"
     CDS             complement(3361..4221)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(4501..4611)
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
                     /note="RK2/RP4 conjugational DNA transfer origin"
BASE COUNT     1024 a      1357 c      1236 g      1171 t 
ORIGIN
        1 tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
       61 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
      121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
      181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
      361 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca 
      421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 
      481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 
      541 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 
      601 acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg 
      661 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 
      721 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 
      781 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 
      841 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 
      901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 
      961 cgctcatgag acaataaccc tgataaatgc ttcaataatc atgcaggtgc tgaaccccca 
     1021 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag 
     1081 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc 
     1141 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc 
     1201 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc 
     1261 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt 
     1321 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag 
     1381 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc 
     1441 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac 
     1501 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg 
     1561 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg 
     1621 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc 
     1681 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt 
     1741 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc 
     1801 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt 
     1861 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg 
     1921 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg 
     1981 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc 
     2041 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg 
     2101 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc 
     2161 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccggaa 
     2221 ttaattcccc ggatcgatcc gtcgatcttg atcccctgcg ccatcagatc cttggcggca 
     2281 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg 
     2341 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc 
     2401 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt 
     2461 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtggctgcca 
     2521 tttttggggt gaggtcgttc gcggccgagg ggcgcagccc ctggggggat ggggtgccgc 
     2581 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg 
     2641 cgccagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta 
     2701 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct 
     2761 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcat cactctgccc 
     2821 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata 
     2881 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca 
     2941 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg 
     3001 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc 
     3061 tcatctgtca gtgagggcca agttttccgc gtggtatcca caacgccggc ggccctacat 
     3121 ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc ctgatccccc gcagaaaaaa 
     3181 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     3241 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     3301 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     3361 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     3421 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     3481 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     3541 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     3601 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     3661 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     3721 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     3781 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     3841 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     3901 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     3961 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 
     4021 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     4081 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     4141 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     4201 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     4261 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     4321 tccgcgcaca tttccccgaa aagtgccacc tgggtcgatc gacggatctt ttccgctgca 
     4381 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 
     4441 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 
     4501 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 
     4561 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaagctcta 
     4621 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 
     4681 tcttcaagaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     4741 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagcaga 
//
