ID pJB3 standard; circular DNA; ; 4788 BP. DE LMBP 3042. CC Accession number: LMBP 3042 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 26-JAN-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 224..307 FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT RBS 308..345 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT /note="5' UTR of the E. coli lacZ gene, incl. ribosome binding site" FT CDS 346..576 FT /vntifkey="4" FT /label=lacZ\fragment FT CDS complement(1044..2192) FT /vntifkey="4" FT /label=trfA FT /note="P382 coding sequence; P285 starts at 1901" FT promoter complement(2301..2350) FT /vntifkey="30" FT /label=Tn5\neo\promoter FT /note="Tn5 neomycin resistance gene promoter" FT rep_origin complement(2590..3114) FT /vntifkey="33" FT /label=RK2\ori FT /note="RK2 vegetative replication origin" FT CDS complement(3361..4221) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(4501..4611) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT /note="RK2/RP4 conjugational DNA transfer origin" SQ Sequence 4788 BP; 1024 A; 1357 C; 1236 G; 1171 t; tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 60 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 120 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 180 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 240 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 300 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 360 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca 420 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 480 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 540 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 600 acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg 660 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 720 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 780 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 840 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 900 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 960 cgctcatgag acaataaccc tgataaatgc ttcaataatc atgcaggtgc tgaaccccca 1020 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag 1080 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc 1140 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc 1200 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc 1260 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt 1320 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag 1380 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc 1440 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac 1500 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg 1560 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg 1620 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc 1680 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt 1740 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc 1800 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt 1860 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg 1920 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg 1980 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc 2040 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg 2100 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc 2160 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccggaa 2220 ttaattcccc ggatcgatcc gtcgatcttg atcccctgcg ccatcagatc cttggcggca 2280 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg 2340 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc 2400 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt 2460 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtggctgcca 2520 tttttggggt gaggtcgttc gcggccgagg ggcgcagccc ctggggggat ggggtgccgc 2580 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg 2640 cgccagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta 2700 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct 2760 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcat cactctgccc 2820 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata 2880 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca 2940 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg 3000 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc 3060 tcatctgtca gtgagggcca agttttccgc gtggtatcca caacgccggc ggccctacat 3120 ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc ctgatccccc gcagaaaaaa 3180 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3240 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3300 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 3360 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 3420 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 3480 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3540 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3600 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3660 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3720 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3780 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3840 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 3900 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 3960 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 4020 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 4080 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 4140 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 4200 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 4260 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 4320 tccgcgcaca tttccccgaa aagtgccacc tgggtcgatc gacggatctt ttccgctgca 4380 taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga 4440 tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag 4500 ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct 4560 tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc gtaagctcta 4620 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 4680 tcttcaagaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 4740 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagcaga 4788 //