LOCUS       pJB3kan1                6070 bp    DNA     circular     11-OCT-2010
DEFINITION  LMBP 3061.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3061
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-OCT-2010
            
            
            
COMMENT     VNTNAME|pJB3kan1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pJB3kan1" 0 6070 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "EcoRI" "HindIII" "XbaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 364 0 1000 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 388 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 3 3505 0 5316 0 5975 0 
COMMENT            0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "224..307") 
COMMENT        #5=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 1 738 1553 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(738..1553)") 
COMMENT        #6=(CFSignal (CObList) "RK2 ori" 33 0 1 3872 4396 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4643 5503 0 
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COMMENT        #8=(CFSignal (CObList) "RK2/RP4 oriT" 33 0 1 5783 5893 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin promoter" 30 0 1 3583 3632 0 
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COMMENT        #10=(CFSignal (CObList) "trfA" 4 0 1 2326 3474 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pJB3kan1'" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6070 bp" 1) 
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COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        224..307
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     CDS             complement(738..1553)
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     CDS             complement(2326..3474)
                     /vntifkey="4"
                     /label=trfA
                     /note="RK2 P382 coding sequence; P285 starts at 3183"
     promoter        complement(3583..3632)
                     /vntifkey="30"
                     /label=Tn5\neomycin\promoter
     rep_origin      complement(3872..4396)
                     /vntifkey="33"
                     /label=RK2\ori
     CDS             complement(4643..5503)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(5783..5893)
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
                     /note="RK2 conjugational DNA transfer origin"
BASE COUNT     1378 a      1666 c      1518 g      1508 t 
ORIGIN
        1 tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
       61 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
      121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
      181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
      361 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcc 
      421 ccggatccgt cgacctgcag gggggggggg gcgctgaggt ctgcctcgtg aagaaggtgt 
      481 tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 
      541 gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 
      601 ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 
      661 atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 
      721 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 
      781 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 
      841 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 
      901 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 
      961 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag 
     1021 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg 
     1081 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa 
     1141 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt 
     1201 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg 
     1261 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata 
     1321 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct 
     1381 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc 
     1441 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg 
     1501 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc 
     1561 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct 
     1621 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttccccccc ccccctgcag 
     1681 gtcgacggat ccggggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 
     1741 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 
     1801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 
     1861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc 
     1921 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca 
     1981 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 
     2041 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 
     2101 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 
     2161 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 
     2221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 
     2281 tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg 
     2341 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca 
     2401 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag 
     2461 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat 
     2521 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc 
     2581 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt 
     2641 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg 
     2701 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt 
     2761 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt 
     2821 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc 
     2881 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg 
     2941 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag 
     3001 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc 
     3061 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg 
     3121 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt 
     3181 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag 
     3241 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac 
     3301 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt 
     3361 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa 
     3421 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc 
     3481 tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct tgatcccctg 
     3541 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc 
     3601 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag 
     3661 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt 
     3721 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga 
     3781 ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga ggggcgcagc 
     3841 ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc 
     3901 cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca aggtttataa 
     3961 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac 
     4021 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc 
     4081 ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag 
     4141 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct 
     4201 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg 
     4261 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc 
     4321 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgtggtatc 
     4381 cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg 
     4441 tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 
     5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga 
     5641 tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt 
     5701 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc 
     5761 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg 
     5821 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct 
     5881 gcgaggctgg ccgtaagctc taagaaacca ttattatcat gacattaacc tataaaaata 
     5941 ggcgtatcac gaggcccttt cgtcttcaag aattaattca ctggccgtcg ttttacaacg 
     6001 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 
     6061 cgccagcaga 
//
