ID pJB3kan2 standard; circular DNA; UNA; 6070 BP. DE LMBP 3062. CC Accession number: LMBP 3062 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 18-JUN-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 224..307 FT /vntifkey="30" FT /label=lac\promoter FT CDS 562..1377 FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT CDS complement(2326..3474) FT /vntifkey="4" FT /label=trfA FT /note="RK2 P382 coding sequence; P285 starts at 3183" FT promoter complement(3583..3632) FT /vntifkey="30" FT /label=Tn5\neomycin\promoter FT rep_origin complement(3872..4396) FT /vntifkey="33" FT /label=RK2\ori FT /note="RK2 vegetative replication origin" FT CDS complement(4643..5503) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(5783..5893) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT /note="RK2/RP4 conjugational DNA transfer origin" SQ Sequence 6070 BP; 1361 A; 1639 C; 1545 G; 1525 t; tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 60 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 120 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 180 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 240 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 300 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 360 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcc 420 ccggatccgt cgacctgcag gggggggggg gaaagccacg ttgtgtctca aaatctctga 480 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata 540 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ctcgaggccg 600 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 660 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt 720 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 780 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 840 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat 900 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 960 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 1020 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 1080 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc 1140 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 1200 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 1260 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 1320 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 1380 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcggc 1440 tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca 1500 acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca 1560 aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcct 1620 ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgccccccc ccccctgcag 1680 gtcgacggat ccggggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 1740 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 1800 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg 1860 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc 1920 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca 1980 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2040 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga 2100 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct 2160 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 2220 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 2280 tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg 2340 caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca 2400 ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag 2460 gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat 2520 ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc 2580 gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt 2640 gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg 2700 gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt 2760 gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt 2820 gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc 2880 gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg 2940 gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag 3000 ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc 3060 gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg 3120 cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt 3180 catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag 3240 acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac 3300 gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt 3360 ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa 3420 ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc 3480 tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct tgatcccctg 3540 cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc 3600 ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag 3660 tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt 3720 tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga 3780 ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga ggggcgcagc 3840 ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc 3900 cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca aggtttataa 3960 atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac 4020 ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc 4080 ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag 4140 tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct 4200 gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg 4260 tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc 4320 ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgtggtatc 4380 cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg 4440 tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4500 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 4560 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 4620 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 4680 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 4740 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 4800 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 4860 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 4920 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 4980 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5040 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5100 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5160 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5220 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 5280 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5340 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 5400 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 5460 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 5520 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 5580 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga 5640 tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt 5700 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc 5760 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg 5820 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct 5880 gcgaggctgg ccgtaagctc taagaaacca ttattatcat gacattaacc tataaaaata 5940 ggcgtatcac gaggcccttt cgtcttcaag aattaattca ctggccgtcg ttttacaacg 6000 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 6060 cgccagcaga 6070 //