ID pJB3tet1 standard; circular DNA; UNA; 6840 BP. DE LMBP 3065. CC Accession number: LMBP 3065 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 18-JUN-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 224..307 FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(1193..2383) FT /vntifkey="4" FT /label=tetracycline\resistance FT CDS complement(3096..4244) FT /vntifkey="4" FT /label=trfA FT /note="RK2 P382 coding sequence; P285 starts at 3953" FT promoter complement(4353..4402) FT /vntifkey="30" FT /label=Tn5\neomycin\promoter FT rep_origin complement(4642..5166) FT /vntifkey="33" FT /label=RK2\ori FT /note="RK2 vegetative replication origin" FT CDS complement(5413..6273) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(6553..6663) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT /note="RK2/RP4 conjugational DNA transfer origin" SQ Sequence 6840 BP; 1489 A; 1919 C; 1862 G; 1570 t; tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 60 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 120 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 180 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 240 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 300 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 360 ccaagcttgc atgcctgcag gtcgactcta gaggatcccc ggctgcggta aagctcatca 420 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt 480 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt 540 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata 600 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta 660 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc 720 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag 780 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc 840 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt 900 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta 960 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt 1020 ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg 1080 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg 1140 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg 1200 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg 1260 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga 1320 cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct 1380 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga 1440 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg 1500 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc 1560 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga 1620 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa 1680 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa 1740 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc 1800 tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta 1860 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc 1920 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga 1980 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa 2040 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atccacagga 2100 cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga 2160 ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca 2220 tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga 2280 tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg 2340 tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc 2400 gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa 2460 catgagaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 2520 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 2580 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg 2640 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 2700 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 2760 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 2820 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 2880 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 2940 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 3000 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tcatgcaggt 3060 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca 3120 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg 3180 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt 3240 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc 3300 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac 3360 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc 3420 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg 3480 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg 3540 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg 3600 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg 3660 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc 3720 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg 3780 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa 3840 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg 3900 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct 3960 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa 4020 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc 4080 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc 4140 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg 4200 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg 4260 attgccccgg aattaattcc ccggatcgat ccgtcgatct tgatcccctg cgccatcaga 4320 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg 4380 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc 4440 gccatgtaag cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc 4500 agatagccca gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct 4560 acgtggctgc catttttggg gtgaggtcgt tcgcggccga ggggcgcagc ccctgggggg 4620 atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg 4680 tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca aggtttataa atattggttt 4740 aaaagcaggt taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat 4800 gctggatttt ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc 4860 atcactctgc ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct 4920 caagtgtcaa taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact 4980 cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg 5040 aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc 5100 aacgtccgcc cctcatctgt cagtgagggc caagttttcc gcgtggtatc cacaacgccg 5160 gcggccctac atggctctgc tgtagtgagt gggttgcgct ccggcagcgg tcctgatccc 5220 ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 5280 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 5340 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 5400 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 5460 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 5520 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 5580 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 5640 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 5700 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 5760 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 5820 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 5880 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 5940 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 6000 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 6060 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 6120 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 6180 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 6240 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 6300 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 6360 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga tcgacggatc 6420 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc 6480 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc 6540 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc 6600 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg 6660 ccgtaagctc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 6720 gaggcccttt cgtcttcaag aattaattca ctggccgtcg ttttacaacg tcgtgactgg 6780 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagcaga 6840 //